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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yggW and yggM are separated by 154 nucleotides
yggM and ansB are separated by 116 nucleotides
ansB and yggN are separated by 175 nucleotides
yggN and yggL are separated by 183 nucleotides
b2955: yggW - coproporphyrinogen III oxidase (NCBI), at 3,095,289 to 3,096,425
yggW
b2956: yggM - hypothetical protein (NCBI), at 3,096,580 to 3,097,587
yggM
b2957: ansB - periplasmic L-asparaginase II (NCBI), at 3,097,704 to 3,098,750
ansB
b2958: yggN - hypothetical protein (NCBI), at 3,098,926 to 3,099,645
yggN
b2959: yggL - orf, hypothetical protein (VIMSS), at 3,099,829 to 3,100,155
yggL
Group
Condition
b2955
b2956
b2957
b2958
b2959
yggW
yggM
ansB
yggN
yggL
phage
Ste41
-0.3
-0.4
-0.6
+0.4
-3.3
carbon source
carbon source D-Glucose 0.25mM
+0.2
-0.4
+0.2
-0.4
-2.5
phage
T7_phage 0.00015625 MOI TC4
-1.1
-0.0
-0.3
-0.1
-0.9
phage
T7_phage 0.00015625 MOI TC2
-1.0
+0.0
-0.2
-0.3
-0.6
phage
T7_phage 0.0015625 MOI TC1
-0.6
+0.1
-0.2
-0.6
-0.5
carbon source
carbon source D-Mannose 0.5mM
+0.0
-0.4
+0.4
-0.5
-1.3
phage
T7_phage 0.00015625 MOI TC3
+0.2
-0.2
-0.1
-1.1
-0.3
control
PBS_cage_1_mice _Feces_16 hr
-0.3
-0.6
-0.0
+0.2
-0.7
predation
6 hr rep2_HD100_10fold_dilution_grewON
-1.0
-0.4
-0.4
+0.0
+0.3
phage
Δdbn_10
-1.2
-0.2
-0.0
-0.3
+0.4
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.3
-0.6
+0.1
+0.2
-0.7
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.9
+0.4
-0.1
+0.5
-0.9
phage
TP4
+0.5
+0.2
-0.0
+0.2
-1.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.4
+0.4
+0.1
-1.2
-0.4
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.7
+0.3
+0.3
-0.2
-0.4
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.8
+0.1
+0.1
-0.4
+0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.1
+0.1
+0.3
+0.9
-0.7
carbon source
carbon source D-Glucose 0.25mM
+0.4
-0.4
-0.2
-0.7
+0.4
stress
Chloramphenicol 0.001 mg/ml
-1.0
+0.1
-0.2
+0.3
+0.4
motility
outer cut, LB soft agar motility assay
-0.4
+0.5
+0.3
+0.3
-0.9
stress
Benzalkonium Chloride 0.004 mg/ml
+0.9
+0.1
+0.1
-0.7
-0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.7
+0.1
-0.5
-0.4
+0.1
phage
Ste41
-0.7
-0.3
-0.1
+0.7
+0.6
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.8
+0.1
+0.1
+0.6
+0.3
predation
3 hr rep1_Tiberius_100fold_dilution
-0.5
-0.3
+0.2
+0.2
+0.8
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.5
+0.1
+0.5
+0.0
+0.8
carbon source
Glycolic Acid (C)
-0.1
+0.8
+0.1
-0.2
+0.5
carbon source
L-Malic (C)
+0.7
+0.7
+0.2
-0.3
-0.2
sir2-hera
SIR2-HerA
+0.3
-0.1
+0.5
+0.6
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
+0.8
+0.1
+0.3
+0.3
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