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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yqfE and argP are separated by 51 nucleotides
argP and yliK are separated by 203 nucleotides
yliK and argK overlap by 8 nucleotides
argK and ygfG are separated by 10 nucleotides
b2915: yqfE - hypothetical protein (RefSeq), at 3,057,403 to 3,057,723
yqfE
b2916: argP - chromosome replication initiation inhibitor protein (NCBI), at 3,057,775 to 3,058,668
argP
b2917: yliK - methylmalonyl-CoA mutase (NCBI), at 3,058,872 to 3,061,016
yliK
b2918: argK - arginine/ornithine transport system ATPase (NCBI), at 3,061,009 to 3,062,004
argK
b2919: ygfG - putative enzyme (VIMSS), at 3,062,015 to 3,062,800
ygfG
Group
Condition
b2915
b2916
b2917
b2918
b2919
yqfE
argP
yliK
argK
ygfG
phage
Ste41
-3.4
-0.4
-0.0
+0.4
-0.3
phage
Ste41
-2.5
-0.1
-0.4
-0.0
-0.8
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-2.0
-0.9
-0.1
+0.2
-0.6
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.1
-0.2
-0.7
-1.9
-0.1
motility
outer cut, LB soft agar motility assay
-1.0
+0.6
+0.2
-2.1
-0.5
control
PBS_cage_2_mouse_2_Cecum_40 hr
-1.1
-1.4
+0.1
-0.3
-0.1
phage
Phage_N4_cage_3_mice _Feces_22 hr
-0.8
-0.9
-0.1
-0.5
-0.1
phage
T7_phage 0.00015625 MOI TC4
-1.2
+0.4
-1.2
-0.0
-0.3
phage
T7_phage 0.00015625 MOI TC3
-0.4
-0.2
-0.2
-1.6
+0.2
control
PBS_cage_1_mice _Feces_16 hr
-1.1
-0.6
-0.2
-0.4
+0.2
phage
Phage_N4_cage_3_mice _Feces_40 hr
+0.0
-1.0
-0.2
-0.8
-0.2
motility
outer cut, LB soft agar motility assay
+0.0
-0.2
+0.2
-1.4
-0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.5
+0.1
-0.2
-1.3
+0.2
motility
outer cut, LB soft agar motility assay
-0.9
+0.5
+0.0
-0.8
-0.2
carbon source
carbon source D-Glucose 0.25mM
-0.7
-1.3
+0.3
+0.1
+0.3
phage
T7_phage 0.0015625 MOI TC1
-1.1
+0.3
-0.7
+0.0
+0.2
phage
Phage_N4_cage_4_mice _Feces_40 hr
+0.5
-1.0
+0.0
-0.4
-0.3
stress
Aluminum chloride 5 mM
-1.1
-0.2
+0.3
+0.4
-0.6
phage
Phage_N4_cage_4_mouse_3_Cecum_40 hr
+0.6
-0.9
-0.0
-0.4
-0.3
phage
T7_phage 0.0015625 MOI TC2
+0.4
-0.4
-0.9
+0.2
-0.2
nitrogen source
Putrescine (N)
-0.4
-0.9
-0.1
+0.3
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.2
+0.3
+0.2
+0.9
-0.7
carbon source
carbon source D-Glucose 0.25mM
+0.4
-0.4
-0.2
+0.6
-0.7
predation
6 hr rep1_HD100_10fold_dilution_grewON
+1.0
-0.5
-0.2
-0.4
-0.1
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.6
-0.1
+0.0
+0.6
-1.2
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
+0.9
-0.7
-0.1
-0.3
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+1.2
-0.1
-0.2
-0.4
-0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.1
+0.3
+0.1
+0.6
-0.6
nitrogen source
L-Arginine (N)
+0.5
+0.8
+0.0
-0.2
+1.0
gfp_control
GFP_control
+0.6
+0.3
+0.3
+0.2
+0.7
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