Fitness for 5 genes in Escherichia coli BW25113

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500 ntyqfE and argP are separated by 51 nucleotidesargP and yliK are separated by 203 nucleotidesyliK and argK overlap by 8 nucleotidesargK and ygfG are separated by 10 nucleotides b2915: yqfE - hypothetical protein (RefSeq), at 3,057,403 to 3,057,723 yqfE b2916: argP - chromosome replication initiation inhibitor protein (NCBI), at 3,057,775 to 3,058,668 argP b2917: yliK - methylmalonyl-CoA mutase (NCBI), at 3,058,872 to 3,061,016 yliK b2918: argK - arginine/ornithine transport system ATPase (NCBI), at 3,061,009 to 3,062,004 argK b2919: ygfG - putative enzyme (VIMSS), at 3,062,015 to 3,062,800 ygfG
Group Condition b2915 b2916 b2917 b2918 b2919
yqfE argP yliK argK ygfG
phage Ste41 -3.4 -0.4 -0.0 +0.4 -0.3
phage Ste41 -2.5 -0.1 -0.4 -0.0 -0.8
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -2.0 -0.9 -0.1 +0.2 -0.6
stress LB Aerobic with Irgasan 0.0000003125 mM -0.1 -0.2 -0.7 -1.9 -0.1
motility outer cut, LB soft agar motility assay -1.0 +0.6 +0.2 -2.1 -0.5
control PBS_cage_2_mouse_2_Cecum_40 hr -1.1 -1.4 +0.1 -0.3 -0.1
phage Phage_N4_cage_3_mice _Feces_22 hr -0.8 -0.9 -0.1 -0.5 -0.1
phage T7_phage 0.00015625 MOI TC4 -1.2 +0.4 -1.2 -0.0 -0.3
phage T7_phage 0.00015625 MOI TC3 -0.4 -0.2 -0.2 -1.6 +0.2
control PBS_cage_1_mice _Feces_16 hr -1.1 -0.6 -0.2 -0.4 +0.2
phage Phage_N4_cage_3_mice _Feces_40 hr +0.0 -1.0 -0.2 -0.8 -0.2
motility outer cut, LB soft agar motility assay +0.0 -0.2 +0.2 -1.4 -0.4
stress LB Aerobic with Ramoplanin 0.000125 mM -0.5 +0.1 -0.2 -1.3 +0.2
motility outer cut, LB soft agar motility assay -0.9 +0.5 +0.0 -0.8 -0.2
carbon source carbon source D-Glucose 0.25mM -0.7 -1.3 +0.3 +0.1 +0.3
phage T7_phage 0.0015625 MOI TC1 -1.1 +0.3 -0.7 +0.0 +0.2
phage Phage_N4_cage_4_mice _Feces_40 hr +0.5 -1.0 +0.0 -0.4 -0.3
stress Aluminum chloride 5 mM -1.1 -0.2 +0.3 +0.4 -0.6
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr +0.6 -0.9 -0.0 -0.4 -0.3
phage T7_phage 0.0015625 MOI TC2 +0.4 -0.4 -0.9 +0.2 -0.2
nitrogen source Putrescine (N) -0.4 -0.9 -0.1 +0.3 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 +0.3 +0.2 +0.9 -0.7
carbon source carbon source D-Glucose 0.25mM +0.4 -0.4 -0.2 +0.6 -0.7
predation 6 hr rep1_HD100_10fold_dilution_grewON +1.0 -0.5 -0.2 -0.4 -0.1
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.6 -0.1 +0.0 +0.6 -1.2
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr +0.9 -0.7 -0.1 -0.3 +0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.2 -0.1 -0.2 -0.4 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.1 +0.3 +0.1 +0.6 -0.6
nitrogen source L-Arginine (N) +0.5 +0.8 +0.0 -0.2 +1.0
gfp_control GFP_control +0.6 +0.3 +0.3 +0.2 +0.7
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