Fitness for 5 genes in Escherichia coli BW25113

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500 ntprfB and recJ are separated by 90 nucleotidesrecJ and dsbC are separated by 5 nucleotidesdsbC and xerD are separated by 24 nucleotidesxerD and fldB are separated by 111 nucleotides b2891: prfB - peptide chain release factor RF-2 (NCBI), at 3,033,206 to 3,034,304 prfB b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128 recJ b2893: dsbC - protein disulfide isomerase II (NCBI), at 3,036,134 to 3,036,844 dsbC b2894: xerD - site-specific tyrosine recombinase XerD (NCBI), at 3,036,869 to 3,037,765 xerD b2895: fldB - flavodoxin 2 (NCBI), at 3,037,877 to 3,038,398 fldB
Group Condition b2891 b2892 b2893 b2894 b2895
prfB recJ dsbC xerD fldB
stress Bacitracin 0.5 mg/ml N.D. -1.4 -4.8 -1.9 +0.6
stress toluene 2.5 vol% N.D. -1.1 -0.6 -3.7 -1.9
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.5 -5.3 -1.0 -0.4
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.6 -4.9 -1.0 -0.5
stress limonene 1.25 vol% N.D. -1.0 -0.8 -3.8 -0.6
stress limonene 1.25 vol% N.D. -0.8 -0.6 -3.8 -0.9
predation 6 hr rep2_HD100_10fold_dilution_grewON N.D. -0.7 -0.4 -2.2 -2.2
phage Phage_N4_cage_3_mice _Feces_22 hr N.D. -0.9 -0.1 -3.4 -1.1
phage Phage_N4_cage_3_mice _Feces_40 hr N.D. -0.9 -0.1 -3.4 -1.0
stress undecene 5 vol% N.D. -1.0 -0.9 -3.1 -0.4
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr N.D. -1.2 -0.3 -1.9 -2.0
motility outer cut, LB soft agar motility assay N.D. -1.1 -0.9 -4.0 +0.7
stress undecene 2.5 vol% N.D. -0.4 -0.5 -3.3 -1.1
ppi ppI N.D. -0.3 -1.3 -1.9 -1.7
stress decyl alcohol 5 vol% N.D. -0.5 -0.4 -3.4 -0.9
stress undecene 2.5 vol% N.D. -0.5 -0.6 -3.7 -0.4
stress LB Aerobic with Nitrofurantoin 0.00003125 mM N.D. -2.9 -0.5 +0.1 -1.9
stress Bacitracin 1 mg/ml N.D. -1.5 -4.1 -0.9 +1.4
stress undecene 5 vol% N.D. -0.9 -1.1 -3.0 -0.2
stress toluene 2.5 vol% N.D. -1.0 -0.4 -2.7 -1.0
stress Cisplatin 0.1 mg/ml N.D. -2.3 -1.1 -0.7 -0.9
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. -1.2 +0.5 -3.0 -1.2
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. -1.3 +0.4 -2.7 -0.8
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. -0.9 +0.5 -2.9 -0.8
control PBS_cage_2_mice _Feces_40 hr N.D. -1.1 +0.7 -2.9 -0.5
motility outer cut, LB soft agar motility assay N.D. -0.7 +0.2 -3.3 +1.4
stress Benzalkonium Chloride 0.004 mg/ml N.D. -0.6 -2.6 -1.1 +2.3
phage T7_phage 0.00015625 MOI TC4 N.D. +1.0 -0.7 +2.7 +1.3
phage T7_phage 0.00015625 MOI TC3 N.D. +2.3 -0.2 +4.6 +0.3
phage T7_phage 0.0015625 MOI TC2 N.D. +2.5 -0.7 +4.7 +1.2
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