Fitness for 5 genes in Escherichia coli BW25113

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500 ntrygA and omrB are separated by 114 nucleotidesomrB and galR are separated by 213 nucleotidesgalR and lysA are separated by 6 nucleotideslysA and lysR are separated by 121 nucleotides b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211 rygA b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407 omrB b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652 galR b2838: lysA - diaminopimelate decarboxylase, PLP-binding (NCBI), at 2,975,659 to 2,976,921 lysA b2839: lysR - DNA-binding transcriptional dual regulator (NCBI), at 2,977,043 to 2,977,978 lysR
Group Condition b4444 b4445 b2837 b2838 b2839
rygA omrB galR lysA lysR
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.5 +0.2 -0.1 -8.7 -9.4
carbon source Ying_sugar7 rep B; time point 3 -0.6 -0.2 +0.3 -8.6 -9.3
carbon source Ying_sugar7 rep C; time point 3 -0.6 -0.4 +0.2 -8.5 -9.2
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.3 -0.3 +0.0 -8.6 -9.3
carbon source Ying_sugar7 rep A; time point 2 -0.6 +0.1 +0.0 -8.3 -9.4
carbon source Ying_sugar7 rep A; time point 3 -0.3 -0.2 +0.3 -8.6 -9.3
carbon source Ying_OrganicAcid7 rep C; time point 3 -0.3 -0.1 +0.0 -8.3 -9.2
carbon source Ying_sugar7 rep C; time point 2 -0.4 -0.1 -0.1 -8.7 -7.8
carbon source Ying_Nucleobase14 rep B; time point 3 -0.3 +0.3 -0.2 -8.3 -6.4
carbon source Ying_Nucleobase14 rep C; time point 3 -0.3 -0.1 -0.5 -8.3 -5.3
carbon source Ying_sugar7 rep B; time point 2 -0.1 +0.1 +0.0 -7.9 -6.4
carbon source Ying_Nucleobase14 rep A; time point 3 -0.4 +0.1 -0.3 -8.6 -4.4
carbon source Ying_OrganicAcid7 rep B; time point 2 +0.1 +0.6 -0.3 -5.8 -8.0
carbon source carbon source D-Glucose 0.25mM -1.2 -1.3 +0.1 -5.4 -4.4
nutrient M9 Glucose 0.2%, Thauer's vit mix -0.1 +0.2 +0.1 -9.2 -3.1
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -0.1 -0.0 +0.0 -8.7 -3.2
carbon source Ying_OrganicAcid7 rep C; time point 2 -0.1 -0.0 -0.1 -6.7 -5.0
nutrient M9 Glucose 0.2%, Thauer's vit mix -0.3 +0.0 +0.1 -8.5 -3.3
carbon source Ying_OrganicAcid7 rep A; time point 2 +0.1 +0.1 -0.0 -6.5 -5.4
carbon source Ying_Nucleobase14 rep C; time point 2 -0.2 -0.2 -0.2 -7.8 -3.4
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -0.0 -0.0 +0.2 -8.3 -3.5
carbon source carbon source D-Mannose 1mM +0.4 -0.7 +0.5 -7.4 -4.5
nitrogen source Gly-DL-Asp (N) -0.2 -0.0 -0.1 -5.9 -5.4
nutrient M9 Glucose 0.2%, Thauer's vit mix -0.2 +0.1 +0.1 -8.5 -3.2
nitrogen source Gly-DL-Asp (N) +0.3 +0.0 +0.1 -5.9 -5.9
carbon source D-Glucose-6-Phosphate (C) -0.1 +0.4 -0.1 -5.9 -5.8
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -0.1 -0.0 -0.3 -7.9 -3.1
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin -0.1 +0.1 +0.1 -8.4 -2.9
carbon source carbon source D-Mannose 0.5mM -0.1 +0.1 +0.3 -7.2 -4.2
carbon source carbon source D-Glucose 0.5mM +0.3 -0.5 +0.2 -5.7 -4.9
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