Fitness for 5 genes in Escherichia coli BW25113

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500 nttas and ygeD are separated by 31 nucleotidesygeD and aas overlap by 8 nucleotidesaas and rygA are separated by 87 nucleotidesrygA and omrB are separated by 114 nucleotides b2834: tas - predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase (NCBI), at 2,969,619 to 2,970,659 tas b2835: ygeD - predicted inner membrane protein (NCBI), at 2,970,691 to 2,971,884 ygeD b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036 aas b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211 rygA b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407 omrB
Group Condition b2834 b2835 b2836 b4444 b4445
tas ygeD aas rygA omrB
phage T7_phage 0.0015625 MOI TC2 -0.3 +0.5 +0.1 -1.3 -2.2
carbon source carbon source D-Glucose 0.25mM -0.1 -0.1 -0.4 -1.2 -1.3
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.1 -0.2 -0.0 -0.3 -1.8
biofilm_assay_planktonic_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None -0.0 -1.2 -1.1 +0.0 -0.1
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 -1.2 -1.2 +0.1 +0.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.2 -0.2 -0.0 -0.9 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.2 -0.1 -1.0 -0.8
phage Bas60 +0.2 -0.3 -0.6 -0.1 -1.1
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 -1.3 -1.2 +0.6 -0.1
motility outer cut, LB soft agar motility assay +0.5 -0.1 +0.1 -1.0 -1.0
carbon source carbon source D-Mannose 5mM -0.5 +0.2 +0.4 -0.5 -0.7
phage Ste41 -0.5 +0.1 -0.0 +0.7 -1.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.0 -0.0 +0.3 +0.3 -1.3
control PBS_cage_1_mice _Feces_22 hr -0.1 +0.2 +0.6 -0.8 -0.6
phage T7_phage 0.00015625 MOI TC4 +0.0 +0.4 -0.4 +0.5 -1.0
phage Δdbn_10 +0.1 -0.1 -0.4 -0.6 +1.1
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.6 -0.7 +0.1 -0.0 +0.7
stress Chloramphenicol 0.001 mg/ml +0.2 +0.2 +0.3 +1.0 -0.4
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.2 +0.3 +0.5 -0.3 +0.9
stress Benzalkonium Chloride 0.004 mg/ml -0.4 +0.7 +0.6 +0.3 +0.7
stress LB Anaerobic with Irgasan 0.000000625 mM +0.1 +0.9 +1.0 -0.1 +0.3
stress LB Anaerobic with Irgasan 0.000000625 mM +0.3 +0.7 +1.2 -0.0 +0.3
phage Papa dilution 10-1 cacl2 maltose +0.5 +0.1 -0.1 +1.1 +0.8
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.3 +1.0 +1.1 +0.1 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM +0.0 +1.4 +1.2 +0.6 -0.4
phage Ste41 +0.4 +0.7 -0.1 +1.5 +0.2
stress LB Aerobic with Ramoplanin 0.000125 mM +0.0 +1.3 +1.3 -0.3 +0.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.4 +1.2 +1.2 +0.2 -0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 +0.5 +1.2 +0.6 +0.5
stress LB Aerobic with Irgasan 0.0000003125 mM +0.5 +5.3 +6.1 -1.0 -0.5
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