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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
tas and ygeD are separated by 31 nucleotides
ygeD and aas overlap by 8 nucleotides
aas and rygA are separated by 87 nucleotides
rygA and omrB are separated by 114 nucleotides
b2834: tas - predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase (NCBI), at 2,969,619 to 2,970,659
tas
b2835: ygeD - predicted inner membrane protein (NCBI), at 2,970,691 to 2,971,884
ygeD
b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036
aas
b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211
rygA
b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407
omrB
Group
Condition
b2834
b2835
b2836
b4444
b4445
tas
ygeD
aas
rygA
omrB
phage
T7_phage 0.0015625 MOI TC2
-0.3
+0.5
+0.1
-1.3
-2.2
carbon source
carbon source D-Glucose 0.25mM
-0.1
-0.1
-0.4
-1.2
-1.3
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.1
-0.2
-0.0
-0.3
-1.8
biofilm_assay_planktonic_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None
-0.0
-1.2
-1.1
+0.0
-0.1
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.1
-1.2
-1.2
+0.1
+0.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.2
-0.2
-0.0
-0.9
-0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
-0.2
-0.1
-1.0
-0.8
phage
Bas60
+0.2
-0.3
-0.6
-0.1
-1.1
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.3
-1.3
-1.2
+0.6
-0.1
motility
outer cut, LB soft agar motility assay
+0.5
-0.1
+0.1
-1.0
-1.0
carbon source
carbon source D-Mannose 5mM
-0.5
+0.2
+0.4
-0.5
-0.7
phage
Ste41
-0.5
+0.1
-0.0
+0.7
-1.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.0
-0.0
+0.3
+0.3
-1.3
control
PBS_cage_1_mice _Feces_22 hr
-0.1
+0.2
+0.6
-0.8
-0.6
phage
T7_phage 0.00015625 MOI TC4
+0.0
+0.4
-0.4
+0.5
-1.0
phage
Δdbn_10
+0.1
-0.1
-0.4
-0.6
+1.1
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.6
-0.7
+0.1
-0.0
+0.7
stress
Chloramphenicol 0.001 mg/ml
+0.2
+0.2
+0.3
+1.0
-0.4
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.2
+0.3
+0.5
-0.3
+0.9
stress
Benzalkonium Chloride 0.004 mg/ml
-0.4
+0.7
+0.6
+0.3
+0.7
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.1
+0.9
+1.0
-0.1
+0.3
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.3
+0.7
+1.2
-0.0
+0.3
phage
Papa dilution 10-1 cacl2 maltose
+0.5
+0.1
-0.1
+1.1
+0.8
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.3
+1.0
+1.1
+0.1
+0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.0
+1.4
+1.2
+0.6
-0.4
phage
Ste41
+0.4
+0.7
-0.1
+1.5
+0.2
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.0
+1.3
+1.3
-0.3
+0.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.4
+1.2
+1.2
+0.2
-0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.3
+0.5
+1.2
+0.6
+0.5
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.5
+5.3
+6.1
-1.0
-0.5
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