Fitness for 5 genes in Escherichia coli BW25113

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500 ntygdR and tas are separated by 107 nucleotidestas and ygeD are separated by 31 nucleotidesygeD and aas overlap by 8 nucleotidesaas and rygA are separated by 87 nucleotides b2833: ygdR - hypothetical protein (NCBI), at 2,969,293 to 2,969,511 ygdR b2834: tas - predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase (NCBI), at 2,969,619 to 2,970,659 tas b2835: ygeD - predicted inner membrane protein (NCBI), at 2,970,691 to 2,971,884 ygeD b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036 aas b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211 rygA
Group Condition b2833 b2834 b2835 b2836 b4444
ygdR tas ygeD aas rygA
biofilm_assay_planktonic_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Biofilm_growth_time=7hrs; Biofilm_growth_media=LB; Sample_type=planktonic; Outgrowth=None -0.1 -0.0 -1.2 -1.1 +0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.1 -0.1 -1.2 -1.2 +0.1
carbon source carbon source D-Glucose 0.25mM -0.4 -0.1 -0.1 -0.4 -1.2
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.3 -1.3 -1.2 +0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 -0.4 -0.5 -0.4 -0.3
phage T7_phage 0.0015625 MOI TC2 -0.1 -0.3 +0.5 +0.1 -1.3
motility outer cut, LB soft agar motility assay -0.4 +0.5 -0.1 +0.1 -1.0
control PBS_cage_1_mouse_1_Cecum_40 hr +0.6 -0.2 -0.2 -0.0 -0.9
carbon source carbon source D-Glucose 5mM -1.1 +0.3 +0.3 -0.1 -0.1
carbon source carbon source D-Mannose 5mM -0.3 -0.5 +0.2 +0.4 -0.5
phage R1_N4_T2_phage8 -0.7 -0.3 +0.1 -0.3 +0.6
stress Cisplatin 0.1 mg/ml -0.7 +0.4 -0.5 +0.2 +0.2
stress LB Anaerobic with Spiramycin 0.000125 mM -0.3 +0.0 +0.5 +0.5 -0.4
stress 2,4-Dinitrophenol 2 mM +0.5 -0.4 +0.1 +0.6 -0.3
control PBS_cage_1_mice _Feces_40 hr +0.6 -0.2 +0.3 +0.4 -0.5
control PBS_cage_1_mice _Feces_22 hr +0.7 -0.1 +0.2 +0.6 -0.8
control PBS_cage_1_mice _Feces_16 hr +0.4 +0.1 +0.3 +0.5 -0.7
stress Bacitracin 1 mg/ml -0.6 -0.1 +0.3 +0.5 +0.8
stress LB Aerobic with Ramoplanin 0.000125 mM -0.8 +0.0 +1.3 +1.3 -0.3
phage Papa dilution 10-1 cacl2 maltose -0.0 +0.5 +0.1 -0.1 +1.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.7 +0.3 +1.0 +1.1 +0.1
stress Chloramphenicol 0.001 mg/ml +0.2 +0.2 +0.2 +0.3 +1.0
phage Ste41 -0.6 +0.4 +0.7 -0.1 +1.5
stress LB Anaerobic with Irgasan 0.000000625 mM +0.0 +0.3 +0.7 +1.2 -0.0
stress LB Anaerobic with Irgasan 0.000000625 mM +0.2 +0.1 +0.9 +1.0 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.6 +0.4 +1.2 +1.2 +0.2
stress Benzalkonium Chloride 0.004 mg/ml +1.4 -0.4 +0.7 +0.6 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM -0.3 +0.0 +1.4 +1.2 +0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.7 +0.3 +0.5 +1.2 +0.6
stress LB Aerobic with Irgasan 0.0000003125 mM -0.6 +0.5 +5.3 +6.1 -1.0
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