Fitness for 5 genes in Escherichia coli BW25113

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500 ntygdK and ygdL are separated by 50 nucleotidesygdL and mltA are separated by 238 nucleotidesmltA and metZ are separated by 208 nucleotidesmetZ and metW are separated by 33 nucleotides b2811: ygdK - predicted Fe-S metabolism protein (NCBI), at 2,942,564 to 2,943,007 ygdK b2812: ygdL - hypothetical protein (NCBI), at 2,943,058 to 2,943,864 ygdL b2813: mltA - membrane-bound lytic murein transglycosylase A (NCBI), at 2,944,103 to 2,945,200 mltA b2814: metZ - tRNA-Met (NCBI), at 2,945,409 to 2,945,485 metZ b2815: metW - tRNA-Met (NCBI), at 2,945,519 to 2,945,595 metW
Group Condition b2811 b2812 b2813 b2814 b2815
ygdK ygdL mltA metZ metW
phage T7_phage 0.0015625 MOI TC2 -2.4 -0.5 -0.1 -5.4 N.D.
carbon source carbon source D-Mannose 0.5mM -0.8 -0.1 +0.3 -6.6 N.D.
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -0.3 +0.0 -6.5 N.D.
stress Cephalothin 0.01 mg/ml +0.1 -0.6 -2.4 -3.2 N.D.
carbon source carbon source D-Glucose 0.25mM +0.4 +0.5 +0.1 -6.6 N.D.
nitrogen source Putrescine (N) -1.0 -1.1 -0.3 -2.0 N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr +0.8 -0.7 +0.1 -4.5 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -2.5 -1.4 -2.7 +2.6 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.5 +0.1 -1.9 -2.7 N.D.
stress Tetracycline 0.0004 mg/ml -0.1 +0.1 +0.1 -4.0 N.D.
stress Cobalt chloride 0.32 mM +0.2 -0.0 -0.1 -4.0 N.D.
motility inner cut, LB soft agar motility assay -1.1 -0.7 +0.2 -1.5 N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.6 -0.2 -2.9 -0.1 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.7 -1.5 -2.8 +2.7 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 -1.4 -3.7 +3.9 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 +0.4 +0.5 -2.4 N.D.
control PBS_cage_1_mouse_2_Cecum_40 hr -1.6 -0.8 -0.1 +1.1 N.D.
phage Ste41 -1.7 -0.5 -0.3 +1.2 N.D.
plasmid transformation vcDART vector transformation; agar assay +0.3 +0.3 +0.2 +2.6 N.D.
carbon source succinate (C) +1.1 +1.0 -0.1 +1.4 N.D.
plasmid transformation vcDART vector transformation; agar assay +0.0 +0.5 +0.2 +2.9 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.6 +0.7 +0.1 +2.4 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.0 +0.9 +0.2 +3.0 N.D.
plasmid transformation vcDART vector transformation; agar assay +0.5 +0.4 +0.1 +3.2 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.9 -0.1 +3.3 N.D.
stress Benzalkonium Chloride 0.004 mg/ml +1.0 +0.9 -0.4 +3.1 N.D.
stress Trimethoprim 0.005 mM +0.1 -0.2 +0.1 +4.7 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.0 +1.6 +0.1 +2.7 N.D.
stress Trimethoprim 0.00125 mM +0.4 +1.0 +0.4 +3.8 N.D.
stress Trimethoprim 0.0025 mM +0.5 +0.9 +0.5 +4.6 N.D.
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