Fitness for 5 genes in Escherichia coli BW25113

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500 ntygdE and ygdD overlap by 8 nucleotidesygdD and gcvA are separated by 18 nucleotidesgcvA and gcvB are separated by 128 nucleotidesgcvB and b2809 are separated by 16 nucleotides b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265 ygdE b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653 ygdD b2808: gcvA - DNA-binding transcriptional dual regulator (NCBI), at 2,939,672 to 2,940,589 gcvA b4443: gcvB - GcvB sRNA gene divergent from gcvA (RefSeq), at 2,940,718 to 2,940,923 gcvB b2809: b2809 - orf, hypothetical protein (VIMSS), at 2,940,940 to 2,941,167 b2809
Group Condition b2806 b2807 b2808 b4443 b2809
ygdE ygdD gcvA gcvB b2809
stress 2,4-Dinitrophenol 2 mM -0.4 -1.3 -4.2 -5.3 -1.4
stress octanol 0.08 vol% -0.5 -1.7 -6.2 -4.7 +0.6
stress 2,4-Dinitrophenol 2 mM -0.4 -1.1 -4.7 -5.3 -0.9
stress decyl alcohol 5 vol% -0.3 +0.2 -1.9 -5.4 -0.4
motility outer cut, LB soft agar motility assay +0.6 +1.6 -5.1 -4.4 -0.3
stress limonene 2.5 vol% -0.1 -0.3 -2.2 -3.9 -0.7
stress toluene 2.5 vol% -0.3 +0.2 -2.6 -3.8 -0.4
stress undecene 5 vol% -0.4 -0.6 -2.6 -2.5 -0.6
stress Bacitracin 1 mg/ml -1.0 -1.0 -2.1 -1.8 -0.8
stress Chloramphenicol 0.001 mg/ml -1.1 -0.2 -2.1 -2.7 -0.2
stress BHIS with Methotrexate 1.98 mM 48 hr -0.3 -0.6 -4.4 +0.1 -1.0
stress undecene 5 vol% -0.4 -0.4 -2.1 -3.0 -0.1
motility outer cut, LB soft agar motility assay +0.7 +1.5 -2.8 -3.3 -2.2
motility inner cut, LB soft agar motility assay +0.0 +0.3 -1.9 -4.0 -0.4
stress BHIS with Methotrexate 1.98 mM 48 hr -0.4 -0.3 -4.4 +0.1 -0.8
no stress control BHIS no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.1 -0.3 -4.3 +0.1 -1.0
stress BHIS with Methotrexate 1.98 mM 48 hr -0.3 -0.6 -4.3 +0.1 -0.7
stress Benzalkonium Chloride 0.004 mg/ml -0.2 +0.3 -2.7 -2.2 -0.8
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.2 -2.2 -3.6 +0.4 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -1.3 -4.9 +0.5 +0.2 +0.0
motility inner cut, LB soft agar motility assay +0.1 +1.2 -0.5 -5.4 -0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 -2.3 -1.8 +0.8 -1.0
motility outer cut, LB soft agar motility assay +0.3 +1.2 -0.4 -5.1 +0.1
stress Cephalothin 0.01 mg/ml -0.4 -2.7 +2.3 -1.0 -0.4
phage Ste41 -0.0 -0.9 +2.6 +1.3 -3.0
phage Ste41 -0.5 +0.1 +2.5 +1.2 -2.1
carbon source Glycolic Acid (C) +0.2 -0.2 +2.5 +2.4 +0.5
carbon source succinate (C) +0.4 -0.4 +2.6 +2.1 +1.1
M9 Acetate 0.5% aerobic +0.2 -0.1 +2.5 +2.7 +0.9
carbon source Glycolic Acid (C) +0.4 +0.3 +2.7 +2.5 +1.0
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