Fitness for 5 genes in Escherichia coli BW25113

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500 ntfucK and fucU are separated by 1 nucleotidesfucU and fucR are separated by 57 nucleotidesfucR and ygdE are separated by 43 nucleotidesygdE and ygdD overlap by 8 nucleotides b2803: fucK - L-fuculokinase (NCBI), at 2,935,460 to 2,936,908 fucK b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332 fucU b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121 fucR b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265 ygdE b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653 ygdD
Group Condition b2803 b2804 b2805 b2806 b2807
fucK fucU fucR ygdE ygdD
stress LB Aerobic with Ramoplanin 0.000125 mM -0.6 -1.8 -0.1 -1.3 -4.9
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -0.4 -0.0 -0.6 -3.7
stress Cephalothin 0.01 mg/ml -0.7 -0.4 -0.5 -0.4 -2.7
phage Ste41 -0.7 -2.5 -0.5 -0.5 +0.1
carbon source L-Fucose (C) -1.8 -0.5 -1.8 +0.1 -0.1
carbon source L-Fucose (C) -2.2 -0.1 -1.8 +0.2 +0.0
stress Aluminum chloride 5 mM -0.4 -2.9 +0.1 -0.4 -0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 -0.7 +0.1 -0.5 -2.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 -1.9 -0.0 -0.6 -1.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 -0.8 -0.4 -0.4 -1.8
phage TP4 +0.3 -2.0 -0.3 -0.1 -1.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 -0.3 +0.0 -0.6 -2.3
stress Carbenicillin 0.008 mg/ml -0.2 -0.9 -0.1 -0.5 -1.4
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +0.1 -1.1 -0.2 -0.3 -1.6
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.3 -0.2 -0.5 -0.2 -2.2
stress Bacitracin 1 mg/ml +0.1 -0.8 +0.1 -1.0 -1.0
phage RB68 -0.2 -0.1 +0.2 -0.3 -2.0
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.4 +0.3 -0.2 -0.1 -1.9
phage RB68 +0.0 -1.0 +0.5 -0.3 -1.4
phage Ste41 -0.9 -0.7 +0.3 -0.0 -0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.7 -0.4 +0.4 -1.7
stress octanol 0.08 vol% -0.4 +0.9 +0.4 -0.5 -1.7
phage Δdbn_10 +0.1 +1.4 +0.3 -0.4 -1.7
carbon source carbon source D-Mannose 0.5mM +0.5 +1.5 +0.2 +0.2 -2.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +1.3 -0.0 +0.1 -1.3
motility inner cut, LB soft agar motility assay -0.0 -0.8 -0.2 +0.3 +1.6
motility outer cut, LB soft agar motility assay +0.1 -0.9 -0.3 +0.7 +1.5
motility outer cut, LB soft agar motility assay -0.1 +0.6 -0.8 +0.6 +1.6
phage phage -0.3 +0.3 +0.1 +0.3 +2.0
stress 3-methyl-1-butanol 1.25 vol% +0.1 +1.2 +0.2 -0.3 +1.5
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