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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fucK and fucU are separated by 1 nucleotides
fucU and fucR are separated by 57 nucleotides
fucR and ygdE are separated by 43 nucleotides
ygdE and ygdD overlap by 8 nucleotides
b2803: fucK - L-fuculokinase (NCBI), at 2,935,460 to 2,936,908
fucK
b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332
fucU
b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121
fucR
b2806: ygdE - predicted methyltransferase (NCBI), at 2,938,165 to 2,939,265
ygdE
b2807: ygdD - conserved inner membrane protein (NCBI), at 2,939,258 to 2,939,653
ygdD
Group
Condition
b2803
b2804
b2805
b2806
b2807
fucK
fucU
fucR
ygdE
ygdD
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.6
-1.8
-0.1
-1.3
-4.9
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
-0.4
-0.0
-0.6
-3.7
stress
Cephalothin 0.01 mg/ml
-0.7
-0.4
-0.5
-0.4
-2.7
phage
Ste41
-0.7
-2.5
-0.5
-0.5
+0.1
carbon source
L-Fucose (C)
-1.8
-0.5
-1.8
+0.1
-0.1
carbon source
L-Fucose (C)
-2.2
-0.1
-1.8
+0.2
+0.0
stress
Aluminum chloride 5 mM
-0.4
-2.9
+0.1
-0.4
-0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
-0.7
+0.1
-0.5
-2.3
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.2
-1.9
-0.0
-0.6
-1.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
-0.8
-0.4
-0.4
-1.8
phage
TP4
+0.3
-2.0
-0.3
-0.1
-1.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.2
-0.3
+0.0
-0.6
-2.3
stress
Carbenicillin 0.008 mg/ml
-0.2
-0.9
-0.1
-0.5
-1.4
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+0.1
-1.1
-0.2
-0.3
-1.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
+0.3
-0.2
-0.5
-0.2
-2.2
stress
Bacitracin 1 mg/ml
+0.1
-0.8
+0.1
-1.0
-1.0
phage
RB68
-0.2
-0.1
+0.2
-0.3
-2.0
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.4
+0.3
-0.2
-0.1
-1.9
phage
RB68
+0.0
-1.0
+0.5
-0.3
-1.4
phage
Ste41
-0.9
-0.7
+0.3
-0.0
-0.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
-0.7
-0.4
+0.4
-1.7
stress
octanol 0.08 vol%
-0.4
+0.9
+0.4
-0.5
-1.7
phage
Δdbn_10
+0.1
+1.4
+0.3
-0.4
-1.7
carbon source
carbon source D-Mannose 0.5mM
+0.5
+1.5
+0.2
+0.2
-2.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+1.3
-0.0
+0.1
-1.3
motility
inner cut, LB soft agar motility assay
-0.0
-0.8
-0.2
+0.3
+1.6
motility
outer cut, LB soft agar motility assay
+0.1
-0.9
-0.3
+0.7
+1.5
motility
outer cut, LB soft agar motility assay
-0.1
+0.6
-0.8
+0.6
+1.6
phage
phage
-0.3
+0.3
+0.1
+0.3
+2.0
stress
3-methyl-1-butanol 1.25 vol%
+0.1
+1.2
+0.2
-0.3
+1.5
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