Fitness for 5 genes in Escherichia coli BW25113

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500 ntfucP and fucI are separated by 32 nucleotidesfucI and fucK are separated by 78 nucleotidesfucK and fucU are separated by 1 nucleotidesfucU and fucR are separated by 57 nucleotides b2801: fucP - L-fucose transporter (NCBI), at 2,932,257 to 2,933,573 fucP b2802: fucI - L-fucose isomerase (NCBI), at 2,933,606 to 2,935,381 fucI b2803: fucK - L-fuculokinase (NCBI), at 2,935,460 to 2,936,908 fucK b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332 fucU b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121 fucR
Group Condition b2801 b2802 b2803 b2804 b2805
fucP fucI fucK fucU fucR
carbon source L-Fucose (C) -1.3 -1.8 -2.2 -0.1 -1.8
carbon source L-Fucose (C) -1.0 -1.6 -1.8 -0.5 -1.8
phage Ste41 -0.2 -0.3 -0.7 -2.5 -0.5
stress Aluminum chloride 5 mM -0.2 +0.2 -0.4 -2.9 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +0.0 -0.6 -1.8 -0.1
phage Ste41 -0.7 -0.2 -0.9 -0.7 +0.3
phage TP4 +0.2 -0.3 +0.3 -2.0 -0.3
protonated/deuterated protonated control -0.0 -0.2 +0.0 -1.5 -0.2
phage T7_phage 0.0015625 MOI TC2 -0.2 -0.3 -0.5 -0.7 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.0 +0.2 -0.2 -1.9 -0.0
stress Cephalothin 0.01 mg/ml -0.1 -0.1 -0.7 -0.4 -0.5
phage RB68 -0.0 -0.5 +0.0 -1.0 +0.5
stress LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM -0.1 +0.2 +0.1 -1.4 +0.2
sir2-hera SIR2-HerA -0.0 +0.1 -0.0 -1.4 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.6 +0.3 -0.7 -0.4
control PBS_cage_1_mouse_1_Cecum_40 hr -0.2 +0.0 +0.2 -1.0 +0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -0.2 -0.6 +0.2 +0.5
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.2 -0.4 -0.5 -0.2 +0.9
mzaabcde mzaABCDE +0.2 +0.3 +0.1 -0.9 +0.5
motility outer cut, LB soft agar motility assay -0.1 +0.5 -0.1 +0.6 -0.8
control LB -0.0 +0.0 -0.4 +1.5 -0.2
stress octanol 0.08 vol% +0.2 -0.1 -0.4 +0.9 +0.4
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.2 -0.4 +0.5 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.2 +0.3 +1.3 -0.0
phage R1_N4_T2_phage8 +0.9 +0.2 +0.0 +0.6 +0.3
control PBS_cage_1_mice _Feces_16 hr +0.1 +0.1 +0.4 +1.0 +0.4
carbon source carbon source D-Mannose 0.5mM +0.2 +0.0 +0.5 +1.5 +0.2
phage Δdbn_10 +0.4 +0.1 +0.1 +1.4 +0.3
carbon source carbon source D-Mannose 1mM -0.1 +0.3 +0.5 +1.4 +0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.1 +0.1 +0.4 +1.8 +0.0
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