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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fucP and fucI are separated by 32 nucleotides
fucI and fucK are separated by 78 nucleotides
fucK and fucU are separated by 1 nucleotides
fucU and fucR are separated by 57 nucleotides
b2801: fucP - L-fucose transporter (NCBI), at 2,932,257 to 2,933,573
fucP
b2802: fucI - L-fucose isomerase (NCBI), at 2,933,606 to 2,935,381
fucI
b2803: fucK - L-fuculokinase (NCBI), at 2,935,460 to 2,936,908
fucK
b2804: fucU - L-fucose mutarotase (NCBI), at 2,936,910 to 2,937,332
fucU
b2805: fucR - DNA-binding transcriptional activator (NCBI), at 2,937,390 to 2,938,121
fucR
Group
Condition
b2801
b2802
b2803
b2804
b2805
fucP
fucI
fucK
fucU
fucR
carbon source
L-Fucose (C)
-1.3
-1.8
-2.2
-0.1
-1.8
carbon source
L-Fucose (C)
-1.0
-1.6
-1.8
-0.5
-1.8
phage
Ste41
-0.2
-0.3
-0.7
-2.5
-0.5
stress
Aluminum chloride 5 mM
-0.2
+0.2
-0.4
-2.9
+0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
+0.0
-0.6
-1.8
-0.1
phage
Ste41
-0.7
-0.2
-0.9
-0.7
+0.3
phage
TP4
+0.2
-0.3
+0.3
-2.0
-0.3
protonated/deuterated
protonated control
-0.0
-0.2
+0.0
-1.5
-0.2
phage
T7_phage 0.0015625 MOI TC2
-0.2
-0.3
-0.5
-0.7
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.0
+0.2
-0.2
-1.9
-0.0
stress
Cephalothin 0.01 mg/ml
-0.1
-0.1
-0.7
-0.4
-0.5
phage
RB68
-0.0
-0.5
+0.0
-1.0
+0.5
stress
LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
-0.1
+0.2
+0.1
-1.4
+0.2
sir2-hera
SIR2-HerA
-0.0
+0.1
-0.0
-1.4
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
+0.6
+0.3
-0.7
-0.4
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.2
+0.0
+0.2
-1.0
+0.4
predation
6 hr rep1_HD100_10fold_dilution_grewON
-0.4
-0.2
-0.6
+0.2
+0.5
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.2
-0.4
-0.5
-0.2
+0.9
mzaabcde
mzaABCDE
+0.2
+0.3
+0.1
-0.9
+0.5
motility
outer cut, LB soft agar motility assay
-0.1
+0.5
-0.1
+0.6
-0.8
control
LB
-0.0
+0.0
-0.4
+1.5
-0.2
stress
octanol 0.08 vol%
+0.2
-0.1
-0.4
+0.9
+0.4
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.3
+0.2
-0.4
+0.5
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
+0.2
+0.3
+1.3
-0.0
phage
R1_N4_T2_phage8
+0.9
+0.2
+0.0
+0.6
+0.3
control
PBS_cage_1_mice _Feces_16 hr
+0.1
+0.1
+0.4
+1.0
+0.4
carbon source
carbon source D-Mannose 0.5mM
+0.2
+0.0
+0.5
+1.5
+0.2
phage
Δdbn_10
+0.4
+0.1
+0.1
+1.4
+0.3
carbon source
carbon source D-Mannose 1mM
-0.1
+0.3
+0.5
+1.4
+0.3
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.1
+0.1
+0.4
+1.8
+0.0
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