Fitness for 5 genes in Escherichia coli BW25113

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500 ntygdG and fucO are separated by 54 nucleotidesfucO and fucA are separated by 27 nucleotidesfucA and fucP are separated by 546 nucleotidesfucP and fucI are separated by 32 nucleotides b2798: ygdG - Ssb-binding protein, misidentified as ExoIX (RefSeq), at 2,929,077 to 2,929,832 ygdG b2799: fucO - L-1,2-propanediol oxidoreductase (NCBI), at 2,929,887 to 2,931,035 fucO b2800: fucA - L-fuculose phosphate aldolase (NCBI), at 2,931,063 to 2,931,710 fucA b2801: fucP - L-fucose transporter (NCBI), at 2,932,257 to 2,933,573 fucP b2802: fucI - L-fucose isomerase (NCBI), at 2,933,606 to 2,935,381 fucI
Group Condition b2798 b2799 b2800 b2801 b2802
ygdG fucO fucA fucP fucI
carbon source L-Fucose (C) -0.3 -1.9 -4.1 -1.3 -1.8
carbon source L-Fucose (C) -0.0 -1.9 -4.1 -1.0 -1.6
control PBS_cage_2_mouse_1_Cecum_40 hr -0.8 -0.1 -1.2 +0.1 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -0.7 +0.1 -0.5 -0.6 -0.2
phage Phage_N4_cage_4_mice _Feces_40 hr -0.5 +0.1 -1.5 +0.1 -0.0
control PBS_cage_2_mice _Feces_40 hr -0.5 +0.3 -1.3 -0.1 -0.1
control PBS_cage_2_mouse_2_Cecum_40 hr -0.4 -0.2 -0.8 +0.0 -0.1
phage T7_phage 0.0015625 MOI TC2 -0.4 -0.9 +0.3 -0.2 -0.3
control PBS_cage_2_mice _Feces_22 hr -0.5 +0.1 -0.8 -0.1 -0.1
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -0.0 +0.1 -1.1 -0.0 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 +0.1 -1.0 -0.6 +0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.6 -0.4 +0.5 -0.2 -0.4
phage T7_phage 0.00015625 MOI TC3 -0.3 -0.4 -0.6 +0.1 +0.1
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.3 -0.1 -1.2 +0.1 -0.1
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr -0.3 +0.2 -1.0 +0.2 -0.1
control PBS_cage_1_mouse_2_Cecum_40 hr +0.3 +0.4 -0.9 -0.3 -0.2
control PBS_cage_1_mice _Feces_40 hr +0.6 +0.4 -1.7 -0.0 +0.1
phage TP4 +0.4 -0.6 -0.5 +0.2 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.2 -0.0 -0.8 -0.1 +0.2
phage Ste41 +0.6 -0.5 -0.0 -0.2 -0.3
carbon source carbon source D-Glucose 0.25mM +0.3 -0.0 -0.8 +0.5 -0.1
phage Ste41 +0.3 +0.4 +0.2 -0.7 -0.2
phage Δdbn_10 +0.3 -0.1 -0.7 +0.4 +0.1
carbon source carbon source D-Mannose 1mM +0.6 +0.1 -0.5 -0.1 +0.3
control PBS_cage_1_mice _Feces_22 hr +0.9 +0.4 -1.0 +0.0 +0.1
control PBS_cage_1_mice _Feces_16 hr +0.9 +0.6 -1.1 +0.1 +0.1
phage R1_N4_T2_phage8 -0.4 -0.1 -0.0 +0.9 +0.2
phage TP4 +0.3 +0.3 -0.5 +0.5 +0.0
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.4 +0.3 +0.2 +0.5 +0.1
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.6 +0.1 +0.4 +0.2 +0.3
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