Fitness for 5 genes in Escherichia coli BW25113

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500 ntgudP and yqcA are separated by 434 nucleotidesyqcA and yqcB are separated by 17 nucleotidesyqcB and yqcC overlap by 1 nucleotidesyqcC and csrB are separated by 42 nucleotides b2789: gudP - predicted D-glucarate transporter (NCBI), at 2,918,770 to 2,920,122 gudP b2790: yqcA - flavodoxin (NCBI), at 2,920,557 to 2,921,006 yqcA b2791: yqcB - tRNA pseudouridine synthase (NCBI), at 2,921,024 to 2,921,806 yqcB b2792: yqcC - hypothetical protein (NCBI), at 2,921,806 to 2,922,135 yqcC b4408: csrB - CsrA-binding sRNA, antagonizing CsrA regulation (NCBI), at 2,922,178 to 2,922,546 csrB
Group Condition b2789 b2790 b2791 b2792 b4408
gudP yqcA yqcB yqcC csrB
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.1 -3.2 +0.4 +0.4 -2.8
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.9 -1.4 -0.5 -0.2 -0.0
carbon source carbon source D-Mannose 0.5mM +0.1 -2.2 -0.0 +0.1 -0.7
carbon source acetate (C) +0.1 -3.6 +0.1 +0.2 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 -3.3 +0.7 +0.5 -0.1
stress LB Aerobic with Ramoplanin 0.000125 mM +0.2 -0.3 -0.1 -1.4 -0.7
control PBS_cage_2_mouse_2_Cecum_40 hr -0.3 -0.0 +0.2 -1.2 -0.8
carbon source acetate (C) +0.0 -3.1 +0.2 +0.1 +0.6
phage T7_phage 0.00015625 MOI TC4 +0.0 +0.3 -0.4 -0.7 -1.2
carbon source Acetate (C) +0.0 -1.7 -0.1 +0.4 -0.2
M9 Acetate 0.5% aerobic +0.0 -1.8 +0.2 +0.3 -0.0
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.1 +0.9 -0.3 -1.9 +0.0
M9 Acetate 0.5% aerobic -0.1 -1.7 +0.2 +0.4 -0.2
carbon source L-Malic (C) +0.0 -1.8 +0.2 -0.1 +0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.0 -0.0 -0.2 -1.6 +0.6
stress Cobalt chloride 0.32 mM -0.2 +0.1 +0.1 -1.2 +0.6
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.4 +0.4 -0.9 +0.4 -0.9
carbon source L-Malic (C) -0.1 -1.8 +0.3 +0.1 +0.8
phage TP4 +0.1 +0.7 +0.1 -0.9 -0.5
stress LB Aerobic with Irgasan 0.0000003125 mM +0.1 -0.4 +0.2 +0.8 -0.7
carbon source carbon source D-Mannose 5mM +0.3 -0.0 +0.2 +1.0 -1.1
stress Benzalkonium Chloride 0.004 mg/ml +0.1 +0.6 +0.6 -1.1 +0.3
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.2 +0.3 +0.2 -0.9 +0.9
motility outer cut, LB soft agar motility assay -0.1 -0.6 -0.8 +0.7 +1.4
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.0 -0.7 -0.3 +0.9 +0.8
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.0 -0.8 -0.3 +1.4 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.6 +0.4 +1.1 +0.1
phage Ste41 -0.5 -1.0 +0.2 +1.0 +1.5
stress octanol 0.08 vol% -0.0 -0.3 +0.2 +1.2 +0.6
stress Chlorite 1.25 mM -0.1 +0.2 +0.4 +0.9 +0.7
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