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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ygcQ and ygcR overlap by 4 nucleotides
ygcR and ygcS overlap by 23 nucleotides
ygcS and ygcU are separated by 93 nucleotides
ygcU and ygcW are separated by 69 nucleotides
b2769: ygcQ - putative flavoprotein (VIMSS), at 2,892,941 to 2,893,801
ygcQ
b2770: ygcR - putative transport protein (VIMSS), at 2,893,798 to 2,894,577
ygcR
b2771: ygcS - putative transport protein (VIMSS), at 2,894,555 to 2,895,892
ygcS
b4463: ygcU - predicted FAD containing dehydrogenase (NCBI), at 2,895,986 to 2,897,440
ygcU
b2774: ygcW - putative oxidoreductase (VIMSS), at 2,897,510 to 2,898,295
ygcW
Group
Condition
b2769
b2770
b2771
b4463
b2774
ygcQ
ygcR
ygcS
ygcU
ygcW
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.0
-0.4
-1.2
+0.1
-0.9
control
NoBALOControl
-0.3
-0.2
-0.3
-0.1
-1.1
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None
-0.2
-0.3
-0.5
-0.2
-0.8
carbon source
carbon source D-Glucose 0.25mM
-1.0
-0.2
-0.4
-0.2
-0.0
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.5
-0.3
+0.2
-0.1
-0.6
carbon source
carbon source D-Mannose 5mM
-0.0
+0.2
-1.0
+0.1
-0.6
stress
Benzalkonium Chloride 0.004 mg/ml
-0.2
+0.2
-0.8
-0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.5
-0.3
+0.3
+0.1
-0.9
balo
Mixed culture, Halobacteriovorax-1
-0.3
-0.6
-0.1
+0.3
-0.5
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-1.0
-0.3
+0.1
-0.2
+0.3
stress
3-methyl-1-butanol 1.25 vol%
-0.9
+0.2
-0.3
-0.2
+0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.9
-0.0
+0.2
-0.3
+0.2
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.7
-0.3
+0.4
+0.2
-0.5
motility
outer cut, LB soft agar motility assay
-0.2
-0.5
+0.6
-0.2
-0.5
nopredatorcontrol
3 hr NoPredatorControl
+0.2
-0.0
+0.2
+0.1
-1.3
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.6
-0.1
+0.0
-0.4
+0.5
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.8
-0.2
+0.5
-0.5
+0.4
phage
T7_phage 0.00015625 MOI TC4
+0.2
+0.4
-0.1
-0.4
-0.7
motility
inner cut, LB soft agar motility assay
+0.6
-0.7
+0.1
-0.3
-0.3
stress
octanol 0.08 vol%
-1.2
+0.0
+0.1
+0.1
+0.5
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.3
-0.1
+0.2
+0.2
-1.0
phage
Papa dilution 10-1 cacl2 maltose
-0.4
-0.3
-0.2
-0.2
+0.8
stress
toluene 2.5 vol%
-0.8
-0.3
+0.6
-0.0
+0.3
phage
T7_phage 0.00015625 MOI TC3
-0.8
-0.1
-0.2
+0.3
+0.7
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.4
+1.2
+0.1
-0.1
stress
Cisplatin 0.1 mg/ml
+0.5
+0.8
-1.1
+0.2
-0.0
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.0
+0.5
+0.3
-0.5
+0.3
stress
Trimethoprim 0.005 mM
+0.0
+0.2
-0.3
-0.2
+1.1
control
PBS_cage_1_mice _Feces_16 hr
+0.5
-0.2
+0.6
+0.1
-0.2
phage
Ste41
+0.6
+0.1
+0.8
+0.0
+0.7
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