Fitness for 5 genes in Escherichia coli BW25113

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500 ntygcQ and ygcR overlap by 4 nucleotidesygcR and ygcS overlap by 23 nucleotidesygcS and ygcU are separated by 93 nucleotidesygcU and ygcW are separated by 69 nucleotides b2769: ygcQ - putative flavoprotein (VIMSS), at 2,892,941 to 2,893,801 ygcQ b2770: ygcR - putative transport protein (VIMSS), at 2,893,798 to 2,894,577 ygcR b2771: ygcS - putative transport protein (VIMSS), at 2,894,555 to 2,895,892 ygcS b4463: ygcU - predicted FAD containing dehydrogenase (NCBI), at 2,895,986 to 2,897,440 ygcU b2774: ygcW - putative oxidoreductase (VIMSS), at 2,897,510 to 2,898,295 ygcW
Group Condition b2769 b2770 b2771 b4463 b2774
ygcQ ygcR ygcS ygcU ygcW
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.0 -0.4 -1.2 +0.1 -0.9
control NoBALOControl -0.3 -0.2 -0.3 -0.1 -1.1
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None -0.2 -0.3 -0.5 -0.2 -0.8
carbon source carbon source D-Glucose 0.25mM -1.0 -0.2 -0.4 -0.2 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM -0.5 -0.3 +0.2 -0.1 -0.6
carbon source carbon source D-Mannose 5mM -0.0 +0.2 -1.0 +0.1 -0.6
stress Benzalkonium Chloride 0.004 mg/ml -0.2 +0.2 -0.8 -0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.5 -0.3 +0.3 +0.1 -0.9
balo Mixed culture, Halobacteriovorax-1 -0.3 -0.6 -0.1 +0.3 -0.5
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -1.0 -0.3 +0.1 -0.2 +0.3
stress 3-methyl-1-butanol 1.25 vol% -0.9 +0.2 -0.3 -0.2 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.9 -0.0 +0.2 -0.3 +0.2
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.7 -0.3 +0.4 +0.2 -0.5
motility outer cut, LB soft agar motility assay -0.2 -0.5 +0.6 -0.2 -0.5
nopredatorcontrol 3 hr NoPredatorControl +0.2 -0.0 +0.2 +0.1 -1.3
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.6 -0.1 +0.0 -0.4 +0.5
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.8 -0.2 +0.5 -0.5 +0.4
phage T7_phage 0.00015625 MOI TC4 +0.2 +0.4 -0.1 -0.4 -0.7
motility inner cut, LB soft agar motility assay +0.6 -0.7 +0.1 -0.3 -0.3
stress octanol 0.08 vol% -1.2 +0.0 +0.1 +0.1 +0.5
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 -0.1 +0.2 +0.2 -1.0
phage Papa dilution 10-1 cacl2 maltose -0.4 -0.3 -0.2 -0.2 +0.8
stress toluene 2.5 vol% -0.8 -0.3 +0.6 -0.0 +0.3
phage T7_phage 0.00015625 MOI TC3 -0.8 -0.1 -0.2 +0.3 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.4 +1.2 +0.1 -0.1
stress Cisplatin 0.1 mg/ml +0.5 +0.8 -1.1 +0.2 -0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.0 +0.5 +0.3 -0.5 +0.3
stress Trimethoprim 0.005 mM +0.0 +0.2 -0.3 -0.2 +1.1
control PBS_cage_1_mice _Feces_16 hr +0.5 -0.2 +0.6 +0.1 -0.2
phage Ste41 +0.6 +0.1 +0.8 +0.0 +0.7
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