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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ygbN and rpoS are separated by 93 nucleotides
rpoS and nlpD are separated by 62 nucleotides
nlpD and pcm are separated by 139 nucleotides
pcm and surE overlap by 7 nucleotides
b2740: ygbN - predicted transporter (NCBI), at 2,863,123 to 2,864,487
ygbN
b2741: rpoS - RNA polymerase sigma factor (NCBI), at 2,864,581 to 2,865,573
rpoS
b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775
nlpD
b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541
pcm
b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296
surE
Group
Condition
b2740
b2741
b2742
b2743
b2744
ygbN
rpoS
nlpD
pcm
surE
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
+1.8
-6.3
-0.9
-2.5
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
+1.4
-5.9
-0.6
-1.5
stress
M9 with Methotrexate 1.98 mM 24 hr
-0.2
-2.0
-3.1
-0.6
-0.5
stress
M9 with Methotrexate 1.98 mM 48 hr
+0.1
-4.0
-0.9
-0.6
-0.8
stress
2,4-Dinitrophenol 2 mM
-0.1
-4.7
-0.6
-1.0
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
-5.0
-0.4
+1.0
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
-4.1
-0.5
+1.2
+0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-4.4
-0.2
+1.8
+0.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
-3.4
-0.0
+2.7
+0.2
carbon source
Ying_OrganicAcid7 rep B; time point 3
+0.0
+6.1
+0.6
-0.5
-0.7
nitrogen source
Gly-Glu (N)
-0.2
+4.8
+0.9
-0.1
+0.0
nitrogen source
Gly-Glu (N)
-0.0
+4.8
+1.1
-0.1
-0.2
carbon source
L-Malic (C)
-0.1
+5.0
+1.3
+0.3
-0.8
carbon source
Ying_OrganicAcid7 rep A; time point 3
+0.1
+6.0
+0.2
-0.2
-0.5
carbon source
L-Malic (C)
+0.1
+4.7
+1.1
+0.2
-0.4
carbon source
Ying_OrganicAcid7 rep C; time point 3
+0.1
+6.2
+0.4
-0.4
-0.4
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
+0.2
+5.4
+1.0
+0.1
-0.2
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
+0.1
+4.8
+1.2
+0.8
-0.0
carbon source
acetate (C)
+0.1
+5.8
+1.1
-0.1
-0.0
carbon source
succinate (C)
+0.1
+5.9
+1.5
+0.1
-0.8
carbon source
succinate (C)
+0.0
+6.4
+1.8
+0.2
-0.9
carbon source
acetate (C)
+0.1
+6.6
+1.1
+0.1
-0.2
carbon source
a-Ketoglutaric (C)
+0.0
+7.0
+1.3
+0.2
-0.4
carbon source
a-Ketoglutaric (C)
+0.0
+7.2
+1.5
+0.4
-0.5
nitrogen source
L-Arginine (N)
+0.0
+9.6
+1.1
+0.5
+0.0
M9 Acetate 0.5% aerobic
+0.1
+9.5
+2.3
-0.0
-0.2
carbon source
Acetate (C)
+0.1
+9.3
+2.9
+0.0
-0.7
nitrogen source
L-Arginine (N)
+0.1
+9.8
+1.2
+0.6
+0.1
carbon source
Acetate (C)
+0.2
+9.3
+2.9
+0.3
-0.5
M9 Acetate 0.5% aerobic
-0.0
+9.9
+2.5
-0.1
-0.0
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