Fitness for 5 genes in Escherichia coli BW25113

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500 ntygbN and rpoS are separated by 93 nucleotidesrpoS and nlpD are separated by 62 nucleotidesnlpD and pcm are separated by 139 nucleotidespcm and surE overlap by 7 nucleotides b2740: ygbN - predicted transporter (NCBI), at 2,863,123 to 2,864,487 ygbN b2741: rpoS - RNA polymerase sigma factor (NCBI), at 2,864,581 to 2,865,573 rpoS b2742: nlpD - predicted outer membrane lipoprotein (NCBI), at 2,865,636 to 2,866,775 nlpD b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541 pcm b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296 surE
Group Condition b2740 b2741 b2742 b2743 b2744
ygbN rpoS nlpD pcm surE
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +1.8 -6.3 -0.9 -2.5
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +1.4 -5.9 -0.6 -1.5
stress M9 with Methotrexate 1.98 mM 24 hr -0.2 -2.0 -3.1 -0.6 -0.5
stress M9 with Methotrexate 1.98 mM 48 hr +0.1 -4.0 -0.9 -0.6 -0.8
stress 2,4-Dinitrophenol 2 mM -0.1 -4.7 -0.6 -1.0 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -5.0 -0.4 +1.0 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -4.1 -0.5 +1.2 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -4.4 -0.2 +1.8 +0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 -3.4 -0.0 +2.7 +0.2
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.0 +6.1 +0.6 -0.5 -0.7
nitrogen source Gly-Glu (N) -0.2 +4.8 +0.9 -0.1 +0.0
nitrogen source Gly-Glu (N) -0.0 +4.8 +1.1 -0.1 -0.2
carbon source L-Malic (C) -0.1 +5.0 +1.3 +0.3 -0.8
carbon source Ying_OrganicAcid7 rep A; time point 3 +0.1 +6.0 +0.2 -0.2 -0.5
carbon source L-Malic (C) +0.1 +4.7 +1.1 +0.2 -0.4
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.1 +6.2 +0.4 -0.4 -0.4
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) +0.2 +5.4 +1.0 +0.1 -0.2
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.1 +4.8 +1.2 +0.8 -0.0
carbon source acetate (C) +0.1 +5.8 +1.1 -0.1 -0.0
carbon source succinate (C) +0.1 +5.9 +1.5 +0.1 -0.8
carbon source succinate (C) +0.0 +6.4 +1.8 +0.2 -0.9
carbon source acetate (C) +0.1 +6.6 +1.1 +0.1 -0.2
carbon source a-Ketoglutaric (C) +0.0 +7.0 +1.3 +0.2 -0.4
carbon source a-Ketoglutaric (C) +0.0 +7.2 +1.5 +0.4 -0.5
nitrogen source L-Arginine (N) +0.0 +9.6 +1.1 +0.5 +0.0
M9 Acetate 0.5% aerobic +0.1 +9.5 +2.3 -0.0 -0.2
carbon source Acetate (C) +0.1 +9.3 +2.9 +0.0 -0.7
nitrogen source L-Arginine (N) +0.1 +9.8 +1.2 +0.6 +0.1
carbon source Acetate (C) +0.2 +9.3 +2.9 +0.3 -0.5
M9 Acetate 0.5% aerobic -0.0 +9.9 +2.5 -0.1 -0.0
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