Fitness for 5 genes in Escherichia coli BW25113

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500 nthycD and hycC are separated by 2 nucleotideshycC and hycB overlap by 4 nucleotideshycB and hycA are separated by 124 nucleotideshycA and hypA are separated by 211 nucleotides b2722: hycD - hydrogenase 3, membrane subunit (NCBI), at 2,844,511 to 2,845,434 hycD b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263 hycC b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871 hycB b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457 hycA b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019 hypA
Group Condition b2722 b2723 b2724 b2725 b2726
hycD hycC hycB hycA hypA
phage T7_phage 0.00015625 MOI TC4 -0.8 +0.2 +0.0 -0.7 -1.0
phage Ste41 -0.1 +0.7 +0.5 -2.6 -0.9
stress Ciprofloxacin Hydrochloride 0.0024 mM +0.1 -0.1 -0.5 -0.2 -1.3
phage T7_phage 0.0015625 MOI TC2 -0.9 -0.4 -0.9 -0.1 +0.4
phage T7_phage 0.0015625 MOI TC1 -0.6 +0.4 -0.1 -0.6 -0.8
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.3 +0.1 -0.1 -0.7 -1.2
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.2 +0.4 -0.5 -0.2 -1.0
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.2 +0.3 +0.3 -0.1 -1.8
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.7 +0.4 -0.7 +0.4 -0.7
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.4 -0.5 +0.2 -0.6 -0.6
motility outer cut, LB soft agar motility assay +0.5 +0.0 -0.2 -0.5 -1.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.5 -0.1 +0.1 -1.2 -0.3
motility outer cut, LB soft agar motility assay +0.6 +0.5 -1.4 +0.2 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.1 +0.2 -1.6 +0.5
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.2 +0.3 +0.4 -0.9 -0.2
stress Chloride 750 mM -0.5 -0.1 -0.4 -0.2 +0.9
carbon source carbon source D-Mannose 0.5mM +0.4 +0.2 +0.4 -0.9 +0.4
stress Cisplatin 0.1 mg/ml +1.0 -0.4 +1.0 -0.4 -0.6
phage RB68 -0.5 +0.7 +0.3 -0.3 +0.5
drt_type_3 DRT_type_3 +0.3 +0.3 +1.0 -0.0 -0.7
gfp_control GFP_control +0.0 +0.4 +1.1 -0.2 -0.4
sir2-hera SIR2-HerA +0.2 +0.4 +1.0 +0.3 -0.7
drt_type_3 DRT_type_3 +0.1 +0.3 +1.2 +0.2 -0.6
retron_ec_48_control retron_Ec_48_control +0.5 +0.3 +1.0 +0.0 -0.4
phage Ste41 -0.6 +1.0 +1.1 -0.7 +0.6
mzaabcde mzaABCDE +0.3 +0.4 +0.9 +0.3 -0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.9 +0.4 -0.6 +0.5 +0.7
sir2-hera SIR2-HerA +0.5 +0.4 +1.3 +0.2 -0.3
drt_type_3 DRT_type_3 +0.3 +0.5 +1.1 +0.4 -0.0
stress Benzalkonium Chloride 0.004 mg/ml +1.5 +0.6 +0.2 +1.4 +0.8
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