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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
hycE and hycD are separated by 17 nucleotides
hycD and hycC are separated by 2 nucleotides
hycC and hycB overlap by 4 nucleotides
hycB and hycA are separated by 124 nucleotides
b2721: hycE - hydrogenase 3, large subunit (NCBI), at 2,842,784 to 2,844,493
hycE
b2722: hycD - hydrogenase 3, membrane subunit (NCBI), at 2,844,511 to 2,845,434
hycD
b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263
hycC
b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871
hycB
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
Group
Condition
b2721
b2722
b2723
b2724
b2725
hycE
hycD
hycC
hycB
hycA
phage
T7_phage 0.0015625 MOI TC2
-0.4
-0.9
-0.4
-0.9
-0.1
phage
Ste41
-0.7
-0.1
+0.7
+0.5
-2.6
phage
phage
-0.4
-0.6
+0.2
-0.3
-0.8
phage
T7_phage 0.00015625 MOI TC4
-0.4
-0.8
+0.2
+0.0
-0.7
phage
T7_phage 0.0015625 MOI TC1
-0.3
-0.6
+0.4
-0.1
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
+0.3
-0.1
+0.2
-1.6
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.2
-0.7
+0.4
-0.7
+0.4
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.0
+0.5
-0.1
+0.1
-1.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.1
-0.2
+0.3
+0.4
-0.9
motility
outer cut, LB soft agar motility assay
-0.2
+0.6
+0.5
-1.4
+0.2
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.1
+0.4
+0.6
-0.5
+0.4
phage
Ste41
-0.1
-0.6
+1.0
+1.1
-0.7
stress
LB Aerobic with Novobiocin 0.0000625 mM
+0.1
+0.4
-0.1
-0.3
+1.1
stress
Cisplatin 0.1 mg/ml
+0.0
+1.0
-0.4
+1.0
-0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.4
+0.6
+0.1
-0.3
+0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.2
+0.9
+0.4
-0.6
+0.5
gfp_control
GFP_control
+0.2
+0.0
+0.4
+1.1
-0.2
gfp_control
GFP_control
+0.1
+0.5
+0.3
+1.0
+0.0
mzaabcde
mzaABCDE
+0.2
+0.1
+0.2
+1.1
+0.3
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.3
+0.3
+0.3
+0.5
+0.5
sir2-hera
SIR2-HerA
+0.1
+0.2
+0.4
+1.0
+0.3
ppi
ppI
+0.3
+0.2
+0.3
+1.0
+0.2
retron_ec_48_control
retron_Ec_48_control
+0.1
+0.3
+0.2
+1.0
+0.4
retron_ec_48_control
retron_Ec_48_control
+0.3
+0.5
+0.3
+1.0
+0.0
retron_ec_48_control
retron_Ec_48_control
+0.3
+0.2
+0.3
+1.0
+0.4
mzaabcde
mzaABCDE
+0.3
+0.3
+0.4
+0.9
+0.3
drt_type_3
DRT_type_3
+0.4
+0.1
+0.3
+1.2
+0.2
sir2-hera
SIR2-HerA
+0.2
+0.5
+0.4
+1.3
+0.2
drt_type_3
DRT_type_3
+0.3
+0.3
+0.5
+1.1
+0.4
stress
Benzalkonium Chloride 0.004 mg/ml
+0.4
+1.5
+0.6
+0.2
+1.4
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