Fitness for 5 genes in Escherichia coli BW25113

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500 nthycE and hycD are separated by 17 nucleotideshycD and hycC are separated by 2 nucleotideshycC and hycB overlap by 4 nucleotideshycB and hycA are separated by 124 nucleotides b2721: hycE - hydrogenase 3, large subunit (NCBI), at 2,842,784 to 2,844,493 hycE b2722: hycD - hydrogenase 3, membrane subunit (NCBI), at 2,844,511 to 2,845,434 hycD b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263 hycC b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871 hycB b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457 hycA
Group Condition b2721 b2722 b2723 b2724 b2725
hycE hycD hycC hycB hycA
phage T7_phage 0.0015625 MOI TC2 -0.4 -0.9 -0.4 -0.9 -0.1
phage Ste41 -0.7 -0.1 +0.7 +0.5 -2.6
phage phage -0.4 -0.6 +0.2 -0.3 -0.8
phage T7_phage 0.00015625 MOI TC4 -0.4 -0.8 +0.2 +0.0 -0.7
phage T7_phage 0.0015625 MOI TC1 -0.3 -0.6 +0.4 -0.1 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 +0.3 -0.1 +0.2 -1.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.2 -0.7 +0.4 -0.7 +0.4
stress LB Aerobic with Irgasan 0.0000003125 mM +0.0 +0.5 -0.1 +0.1 -1.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 -0.2 +0.3 +0.4 -0.9
motility outer cut, LB soft agar motility assay -0.2 +0.6 +0.5 -1.4 +0.2
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr -0.1 +0.4 +0.6 -0.5 +0.4
phage Ste41 -0.1 -0.6 +1.0 +1.1 -0.7
stress LB Aerobic with Novobiocin 0.0000625 mM +0.1 +0.4 -0.1 -0.3 +1.1
stress Cisplatin 0.1 mg/ml +0.0 +1.0 -0.4 +1.0 -0.4
stress LB Aerobic with Ramoplanin 0.000125 mM +0.4 +0.6 +0.1 -0.3 +0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 +0.9 +0.4 -0.6 +0.5
gfp_control GFP_control +0.2 +0.0 +0.4 +1.1 -0.2
gfp_control GFP_control +0.1 +0.5 +0.3 +1.0 +0.0
mzaabcde mzaABCDE +0.2 +0.1 +0.2 +1.1 +0.3
stress LB Anaerobic with Spiramycin 0.000125 mM +0.3 +0.3 +0.3 +0.5 +0.5
sir2-hera SIR2-HerA +0.1 +0.2 +0.4 +1.0 +0.3
ppi ppI +0.3 +0.2 +0.3 +1.0 +0.2
retron_ec_48_control retron_Ec_48_control +0.1 +0.3 +0.2 +1.0 +0.4
retron_ec_48_control retron_Ec_48_control +0.3 +0.5 +0.3 +1.0 +0.0
retron_ec_48_control retron_Ec_48_control +0.3 +0.2 +0.3 +1.0 +0.4
mzaabcde mzaABCDE +0.3 +0.3 +0.4 +0.9 +0.3
drt_type_3 DRT_type_3 +0.4 +0.1 +0.3 +1.2 +0.2
sir2-hera SIR2-HerA +0.2 +0.5 +0.4 +1.3 +0.2
drt_type_3 DRT_type_3 +0.3 +0.3 +0.5 +1.1 +0.4
stress Benzalkonium Chloride 0.004 mg/ml +0.4 +1.5 +0.6 +0.2 +1.4
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