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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
gutM and srlR are separated by 66 nucleotides
srlR and gutQ overlap by 8 nucleotides
gutQ and ygaA overlap by 4 nucleotides
ygaA and norV are separated by 186 nucleotides
b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002
gutM
b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842
srlR
b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800
gutQ
b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311
ygaA
b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937
norV
Group
Condition
b2706
b2707
b2708
b2709
b2710
gutM
srlR
gutQ
ygaA
norV
stress
2,4-Dinitrophenol 2 mM
+0.4
-3.2
-0.3
-0.1
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
-2.9
-0.4
-0.0
-0.4
stress
2,4-Dinitrophenol 2 mM
+0.4
-3.3
-0.3
+0.1
-0.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
-3.2
-0.1
+0.2
+0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.1
-2.5
-0.6
+0.4
-0.4
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.0
-0.9
-1.1
+0.3
-0.3
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.3
-2.5
-0.5
-0.0
-0.3
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.0
-2.6
-0.4
-0.1
+0.1
stress
Benzalkonium Chloride 0.004 mg/ml
+0.2
-1.3
-1.0
-0.3
-0.1
carbon source
a-Ketoglutaric (C)
+0.2
-1.8
-0.5
+0.1
-0.4
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.0
-2.3
-0.4
+0.4
-0.1
carbon source
Glycolic Acid (C)
+0.1
-1.8
-0.5
+0.2
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
-1.8
-0.5
-0.3
-0.2
carbon source
carbon source D-Mannose 0.5mM
-1.1
-1.2
+0.3
-0.1
-0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
-1.9
-0.6
+0.0
+0.0
carbon source
carbon source D-Mannose 0.5mM
+0.3
-1.9
-0.3
+0.0
+0.2
carbon source
carbon source D-Glucose 20mM
-1.7
+0.5
-0.1
-0.2
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.7
-0.7
-0.9
-0.1
-0.5
motility
outer cut, LB soft agar motility assay
+0.9
-1.2
-1.1
+0.1
+0.3
stress
3-methyl-1-butanol 1.25 vol%
-1.5
+0.5
+0.2
-0.1
+0.3
phage
TP4
+0.2
-1.3
+0.9
+0.3
+0.0
phage
Ste41
-0.6
+1.0
+0.5
-0.5
+0.0
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
-0.1
+1.5
-0.6
-0.4
+0.1
stress
Carbenicillin 0.008 mg/ml
-0.7
+1.2
+0.5
-0.1
+0.1
phage
Phage_N4_cage_4_mouse_2_Cecum_40 hr
+0.1
+2.1
-0.9
-0.2
+0.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.6
+0.8
+1.3
-0.3
+0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.4
+0.2
+2.4
-0.0
-0.1
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.4
+0.1
+1.9
+0.4
+0.0
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.6
+0.3
+1.8
-0.2
-0.0
stress
LB Aerobic with Nitrofurantoin 0.00003125 mM
+0.2
+0.3
+2.4
+0.1
-0.0
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