Fitness for 5 genes in Escherichia coli BW25113

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500 ntgutM and srlR are separated by 66 nucleotidessrlR and gutQ overlap by 8 nucleotidesgutQ and ygaA overlap by 4 nucleotidesygaA and norV are separated by 186 nucleotides b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002 gutM b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842 srlR b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800 gutQ b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311 ygaA b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937 norV
Group Condition b2706 b2707 b2708 b2709 b2710
gutM srlR gutQ ygaA norV
stress 2,4-Dinitrophenol 2 mM +0.4 -3.2 -0.3 -0.1 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -2.9 -0.4 -0.0 -0.4
stress 2,4-Dinitrophenol 2 mM +0.4 -3.3 -0.3 +0.1 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 -3.2 -0.1 +0.2 +0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -2.5 -0.6 +0.4 -0.4
stress LB Aerobic with Irgasan 0.0000003125 mM -1.0 -0.9 -1.1 +0.3 -0.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 -2.5 -0.5 -0.0 -0.3
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.0 -2.6 -0.4 -0.1 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.2 -1.3 -1.0 -0.3 -0.1
carbon source a-Ketoglutaric (C) +0.2 -1.8 -0.5 +0.1 -0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.0 -2.3 -0.4 +0.4 -0.1
carbon source Glycolic Acid (C) +0.1 -1.8 -0.5 +0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 -1.8 -0.5 -0.3 -0.2
carbon source carbon source D-Mannose 0.5mM -1.1 -1.2 +0.3 -0.1 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 -1.9 -0.6 +0.0 +0.0
carbon source carbon source D-Mannose 0.5mM +0.3 -1.9 -0.3 +0.0 +0.2
carbon source carbon source D-Glucose 20mM -1.7 +0.5 -0.1 -0.2 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 -0.7 -0.9 -0.1 -0.5
motility outer cut, LB soft agar motility assay +0.9 -1.2 -1.1 +0.1 +0.3
stress 3-methyl-1-butanol 1.25 vol% -1.5 +0.5 +0.2 -0.1 +0.3
phage TP4 +0.2 -1.3 +0.9 +0.3 +0.0
phage Ste41 -0.6 +1.0 +0.5 -0.5 +0.0
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -0.1 +1.5 -0.6 -0.4 +0.1
stress Carbenicillin 0.008 mg/ml -0.7 +1.2 +0.5 -0.1 +0.1
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr +0.1 +2.1 -0.9 -0.2 +0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.6 +0.8 +1.3 -0.3 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.4 +0.2 +2.4 -0.0 -0.1
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.4 +0.1 +1.9 +0.4 +0.0
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.6 +0.3 +1.8 -0.2 -0.0
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.2 +0.3 +2.4 +0.1 -0.0
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