Fitness for 5 genes in Escherichia coli BW25113

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500 ntsrlE and srlB are separated by 10 nucleotidessrlB and srlD are separated by 3 nucleotidessrlD and gutM are separated by 104 nucleotidesgutM and srlR are separated by 66 nucleotides b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373 srlE b2704: srlB - glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI), at 2,825,384 to 2,825,755 srlB b2705: srlD - 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI), at 2,825,759 to 2,826,538 srlD b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002 gutM b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842 srlR
Group Condition b2703 b2704 b2705 b2706 b2707
srlE srlB srlD gutM srlR
carbon source D-Sorbitol (C) -1.6 -1.0 -5.4 -0.1 +0.0
carbon source D-Sorbitol (C) -1.1 -0.6 -4.7 -0.2 +0.1
carbon source carbon source D-Mannose 0.5mM -0.9 -1.9 +0.4 +0.3 -1.9
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.0 -1.2 -0.2 +0.3 -2.5
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.4 -0.0 -0.1 -0.0 -2.6
stress octanol 0.08 vol% +0.3 -1.5 -0.5 -0.7 -0.7
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.2 -0.2 -0.3 +0.0 -2.3
stress 2,4-Dinitrophenol 2 mM +0.1 -0.1 +0.2 +0.4 -3.3
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.6 -1.3 +0.4 -0.8 -0.1
carbon source carbon source D-Mannose 0.5mM -0.2 -0.2 +0.3 -1.1 -1.2
carbon source carbon source D-Mannose 5mM +0.1 -1.4 +0.5 -0.6 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 +0.8 +0.5 +0.0 -3.2
stress 2,4-Dinitrophenol 2 mM +0.1 +0.4 +0.3 +0.4 -3.2
phage RB68 -0.5 -1.0 -0.5 -0.5 +0.5
stress LB Aerobic with Irgasan 0.0000003125 mM +0.4 -0.5 +0.3 -1.0 -0.9
motility outer cut, LB soft agar motility assay -0.2 -1.0 -0.2 +0.9 -1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +0.6 +0.1 -0.1 -2.5
motility outer cut, LB soft agar motility assay +0.6 -0.6 -0.2 -0.8 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +1.0 +0.0 +0.4 -2.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 +0.5 -0.4 +0.6 -1.8
carbon source carbon source D-Glucose 20mM -0.3 +0.4 -0.5 -1.7 +0.5
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -0.2 -0.6 +0.8 -0.4 -1.1
carbon source Glycolic Acid (C) -0.3 +0.5 +0.4 +0.1 -1.8
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.4 +0.2 +0.4 -1.9
stress Cisplatin 0.1 mg/ml +0.3 -0.8 +0.9 -0.8 -0.6
stress Cephalothin 0.01 mg/ml +0.3 -1.3 -0.1 +0.3 +1.6
phage Phage_N4_cage_4_mouse_2_Cecum_40 hr -0.0 -0.5 -0.5 +0.1 +2.1
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr +0.6 -0.3 -0.6 -0.1 +1.5
stress Carbenicillin 0.008 mg/ml -0.0 +0.8 -0.0 -0.7 +1.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +1.2 +1.5 -0.6 +0.5 +0.0
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