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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
srlE and srlB are separated by 10 nucleotides
srlB and srlD are separated by 3 nucleotides
srlD and gutM are separated by 104 nucleotides
gutM and srlR are separated by 66 nucleotides
b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373
srlE
b2704: srlB - glucitol/sorbitol-specific enzyme IIA component of PTS (NCBI), at 2,825,384 to 2,825,755
srlB
b2705: srlD - 3-ketoacyl-(acyl-carrier-protein) reductase (NCBI), at 2,825,759 to 2,826,538
srlD
b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002
gutM
b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842
srlR
Group
Condition
b2703
b2704
b2705
b2706
b2707
srlE
srlB
srlD
gutM
srlR
carbon source
D-Sorbitol (C)
-1.6
-1.0
-5.4
-0.1
+0.0
carbon source
D-Sorbitol (C)
-1.1
-0.6
-4.7
-0.2
+0.1
carbon source
carbon source D-Mannose 0.5mM
-0.9
-1.9
+0.4
+0.3
-1.9
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.0
-1.2
-0.2
+0.3
-2.5
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.4
-0.0
-0.1
-0.0
-2.6
stress
octanol 0.08 vol%
+0.3
-1.5
-0.5
-0.7
-0.7
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-0.2
-0.2
-0.3
+0.0
-2.3
stress
2,4-Dinitrophenol 2 mM
+0.1
-0.1
+0.2
+0.4
-3.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.6
-1.3
+0.4
-0.8
-0.1
carbon source
carbon source D-Mannose 0.5mM
-0.2
-0.2
+0.3
-1.1
-1.2
carbon source
carbon source D-Mannose 5mM
+0.1
-1.4
+0.5
-0.6
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.1
+0.8
+0.5
+0.0
-3.2
stress
2,4-Dinitrophenol 2 mM
+0.1
+0.4
+0.3
+0.4
-3.2
phage
RB68
-0.5
-1.0
-0.5
-0.5
+0.5
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.4
-0.5
+0.3
-1.0
-0.9
motility
outer cut, LB soft agar motility assay
-0.2
-1.0
-0.2
+0.9
-1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
+0.6
+0.1
-0.1
-2.5
motility
outer cut, LB soft agar motility assay
+0.6
-0.6
-0.2
-0.8
-0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
+1.0
+0.0
+0.4
-2.9
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
+0.5
-0.4
+0.6
-1.8
carbon source
carbon source D-Glucose 20mM
-0.3
+0.4
-0.5
-1.7
+0.5
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.2
-0.6
+0.8
-0.4
-1.1
carbon source
Glycolic Acid (C)
-0.3
+0.5
+0.4
+0.1
-1.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
+0.4
+0.2
+0.4
-1.9
stress
Cisplatin 0.1 mg/ml
+0.3
-0.8
+0.9
-0.8
-0.6
stress
Cephalothin 0.01 mg/ml
+0.3
-1.3
-0.1
+0.3
+1.6
phage
Phage_N4_cage_4_mouse_2_Cecum_40 hr
-0.0
-0.5
-0.5
+0.1
+2.1
phage
Phage_N4_cage_4_mouse_1_Cecum_40 hr
+0.6
-0.3
-0.6
-0.1
+1.5
stress
Carbenicillin 0.008 mg/ml
-0.0
+0.8
-0.0
-0.7
+1.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+1.2
+1.5
-0.6
+0.5
+0.0
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