Fitness for 5 genes in Escherichia coli BW25113

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500 ntmprA and emrA are separated by 126 nucleotidesemrA and emrB are separated by 16 nucleotidesemrB and luxS are separated by 63 nucleotidesluxS and micA are separated by 68 nucleotides b2684: mprA - DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI), at 2,808,792 to 2,809,322 mprA b2685: emrA - multidrug efflux system (NCBI), at 2,809,449 to 2,810,621 emrA b2686: emrB - multidrug efflux system protein (NCBI), at 2,810,638 to 2,812,176 emrB b2687: luxS - S-ribosylhomocysteinase (NCBI), at 2,812,240 to 2,812,755 luxS b4442: micA - sRNA effector of ompA mRNA instability in stationary phase; requires Hfq (RefSeq), at 2,812,824 to 2,812,901 micA
Group Condition b2684 b2685 b2686 b2687 b4442
mprA emrA emrB luxS micA
stress 2,4-Dinitrophenol 2 mM +1.5 -4.7 -5.5 -0.3 N.D.
stress 2,4-Dinitrophenol 2 mM +1.7 -3.7 -4.1 -0.3 N.D.
stress 2,4-Dinitrophenol 1 mM +1.8 -3.4 -3.5 -0.0 N.D.
stress 2,4-Dinitrophenol 1 mM +1.7 -3.0 -3.0 -0.2 N.D.
carbon source Glycolic Acid (C) -3.0 +0.6 +0.8 -0.9 N.D.
carbon source Glycolic Acid (C) -2.4 +0.6 +0.9 -0.9 N.D.
stress M9 with Methotrexate 1.98 mM 24 hr +3.8 -2.6 -2.9 -0.0 N.D.
stress M9 with Methotrexate 1.98 mM 24 hr +2.7 -1.9 -2.2 -0.1 N.D.
stress M9 with Methotrexate 1.98 mM 48 hr +2.8 -1.8 -2.2 +0.1 N.D.
stress M9 with Methotrexate 1.98 mM 48 hr +4.2 -2.9 -2.4 -0.0 N.D.
stress M9 with Methotrexate 1.98 mM 24 hr +6.2 -2.6 -2.9 -0.1 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +3.2 -1.1 -1.2 +0.1 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x +3.2 -0.8 -0.9 +0.0 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x +3.1 -0.6 -0.6 +0.2 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +4.6 -1.1 -1.2 -0.1 N.D.
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x +4.4 -1.1 -0.7 +0.0 N.D.
stress Ciprofloxacin Hydrochloride 0.0024 mM +4.3 -1.1 -0.3 -0.2 N.D.
supernatant Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x; and D-Glucose 22.2 mM +3.6 -0.3 -0.3 -0.1 N.D.
stress Nalidixic 0.006 mg/ml +6.8 -1.8 -1.5 +0.0 N.D.
stress octanol 0.08 vol% +4.1 +0.2 -0.5 -0.2 N.D.
stress Trimethoprim 0.0025 mM +3.3 +0.5 +0.5 +0.1 N.D.
motility outer cut, LB soft agar motility assay +3.8 +0.2 +0.2 +0.1 N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM +3.5 +0.7 +0.6 -0.2 N.D.
stress LB Anaerobic with Nalidixic 0.0000078125 mM +5.5 -0.3 -0.3 -0.1 N.D.
stress LB Anaerobic with Nalidixic 0.0000078125 mM +6.1 -0.3 -0.4 -0.5 N.D.
stress Nalidixic 0.0313 mM +6.5 -0.4 -0.4 -0.0 N.D.
stress Nalidixic 0.06 mM +7.7 -0.9 -1.0 -0.1 N.D.
stress Benzalkonium Chloride 0.004 mg/ml +3.1 +1.6 +1.1 +0.2 N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM +4.5 +0.8 +0.9 -0.1 N.D.
stress Nalidixic 0.06 mM +8.6 -0.7 -0.7 -0.2 N.D.
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