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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yqaE and ygaV are separated by 182 nucleotides
ygaV and ygaP are separated by 9 nucleotides
ygaP and stpA are separated by 46 nucleotides
stpA and ygaW are separated by 668 nucleotides
b2666: yqaE - predicted membrane protein (NCBI), at 2,794,892 to 2,795,050
yqaE
b2667: ygaV - predicted DNA-binding transcriptional regulator (NCBI), at 2,795,233 to 2,795,532
ygaV
b2668: ygaP - predicted inner membrane protein with hydrolase activity (NCBI), at 2,795,542 to 2,796,066
ygaP
b2669: stpA - DNA binding protein, nucleoid-associated (NCBI), at 2,796,113 to 2,796,517
stpA
b2670: ygaW - predicted inner membrane protein (NCBI), at 2,797,186 to 2,797,635
ygaW
Group
Condition
b2666
b2667
b2668
b2669
b2670
yqaE
ygaV
ygaP
stpA
ygaW
carbon source
carbon source D-Glucose 0.25mM
-1.2
-0.8
-0.2
+0.1
-1.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.4
-0.4
-0.2
-0.2
-0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.2
-0.2
-0.0
-0.3
-0.7
phage
T7_phage 0.00015625 MOI TC4
-1.0
-0.6
-0.5
+0.4
-0.1
balo
Mixed culture, Halobacteriovorax-10
-1.3
+0.3
+0.2
-0.2
-0.7
motility
outer cut, LB soft agar motility assay
-1.4
+0.2
+0.0
-0.4
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.9
+0.3
-0.2
-0.6
-0.1
stress
LB Aerobic with Novobiocin 0.0000625 mM
-1.1
-0.5
-0.4
+0.1
+0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.0
+0.5
-0.5
-0.4
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.0
+0.3
+0.0
-0.3
-0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.0
+0.4
-0.3
-0.7
-0.7
nitrogen source
L-Alanine (N)
+0.3
+0.0
+0.0
-0.1
-1.6
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
+0.2
-0.9
-0.2
-0.6
nitrogen source
Putrescine (N)
+0.5
-0.4
+0.2
-0.9
-0.0
phage
Lambda_ci1857
-1.1
-0.5
-0.3
+0.8
+0.4
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
-0.4
+0.2
+0.4
-0.6
-0.3
no stress control
M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
-0.1
+0.4
+0.6
-0.7
-0.4
carbon source
Acetate (C)
-0.2
+0.5
+0.5
-0.7
-0.2
carbon source
Glycolic Acid (C)
+1.2
-0.0
+0.3
-0.7
-0.2
carbon source
Acetate (C)
+0.6
+0.4
+0.6
-0.7
-0.1
stress
BHIS with Methotrexate 0.06 mM 24 hr
+0.6
+0.2
+0.5
-0.6
+0.0
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.9
+0.4
-0.3
-0.3
+0.3
carbon source
L-Malic (C)
+0.9
+0.4
+0.3
-0.7
+0.1
phage
R1_N4_T2_phage8
+0.6
+0.5
+0.4
-0.5
+0.0
carbon source
succinate (C)
+0.8
+0.2
+0.4
-0.5
+0.1
carbon source
a-Ketoglutaric (C)
+0.6
+0.5
+0.4
-0.4
+0.1
carbon source
Glycolic Acid (C)
+1.0
+0.1
+0.5
-0.6
+0.2
carbon source
L-Malic (C)
+0.9
+0.4
+0.5
-0.6
+0.3
carbon source
succinate (C)
+0.9
+0.3
+0.5
-0.6
+0.3
lb
LB
+0.5
+0.8
+0.3
-0.3
+0.6
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