Fitness for 5 genes in Escherichia coli BW25113

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500 ntyqaE and ygaV are separated by 182 nucleotidesygaV and ygaP are separated by 9 nucleotidesygaP and stpA are separated by 46 nucleotidesstpA and ygaW are separated by 668 nucleotides b2666: yqaE - predicted membrane protein (NCBI), at 2,794,892 to 2,795,050 yqaE b2667: ygaV - predicted DNA-binding transcriptional regulator (NCBI), at 2,795,233 to 2,795,532 ygaV b2668: ygaP - predicted inner membrane protein with hydrolase activity (NCBI), at 2,795,542 to 2,796,066 ygaP b2669: stpA - DNA binding protein, nucleoid-associated (NCBI), at 2,796,113 to 2,796,517 stpA b2670: ygaW - predicted inner membrane protein (NCBI), at 2,797,186 to 2,797,635 ygaW
Group Condition b2666 b2667 b2668 b2669 b2670
yqaE ygaV ygaP stpA ygaW
carbon source carbon source D-Glucose 0.25mM -1.2 -0.8 -0.2 +0.1 -1.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.4 -0.4 -0.2 -0.2 -0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.2 -0.2 -0.0 -0.3 -0.7
phage T7_phage 0.00015625 MOI TC4 -1.0 -0.6 -0.5 +0.4 -0.1
balo Mixed culture, Halobacteriovorax-10 -1.3 +0.3 +0.2 -0.2 -0.7
motility outer cut, LB soft agar motility assay -1.4 +0.2 +0.0 -0.4 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 +0.3 -0.2 -0.6 -0.1
stress LB Aerobic with Novobiocin 0.0000625 mM -1.1 -0.5 -0.4 +0.1 +0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.0 +0.5 -0.5 -0.4 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.0 +0.3 +0.0 -0.3 -0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.0 +0.4 -0.3 -0.7 -0.7
nitrogen source L-Alanine (N) +0.3 +0.0 +0.0 -0.1 -1.6
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +0.2 -0.9 -0.2 -0.6
nitrogen source Putrescine (N) +0.5 -0.4 +0.2 -0.9 -0.0
phage Lambda_ci1857 -1.1 -0.5 -0.3 +0.8 +0.4
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr -0.4 +0.2 +0.4 -0.6 -0.3
no stress control M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr -0.1 +0.4 +0.6 -0.7 -0.4
carbon source Acetate (C) -0.2 +0.5 +0.5 -0.7 -0.2
carbon source Glycolic Acid (C) +1.2 -0.0 +0.3 -0.7 -0.2
carbon source Acetate (C) +0.6 +0.4 +0.6 -0.7 -0.1
stress BHIS with Methotrexate 0.06 mM 24 hr +0.6 +0.2 +0.5 -0.6 +0.0
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.9 +0.4 -0.3 -0.3 +0.3
carbon source L-Malic (C) +0.9 +0.4 +0.3 -0.7 +0.1
phage R1_N4_T2_phage8 +0.6 +0.5 +0.4 -0.5 +0.0
carbon source succinate (C) +0.8 +0.2 +0.4 -0.5 +0.1
carbon source a-Ketoglutaric (C) +0.6 +0.5 +0.4 -0.4 +0.1
carbon source Glycolic Acid (C) +1.0 +0.1 +0.5 -0.6 +0.2
carbon source L-Malic (C) +0.9 +0.4 +0.5 -0.6 +0.3
carbon source succinate (C) +0.9 +0.3 +0.5 -0.6 +0.3
lb LB +0.5 +0.8 +0.3 -0.3 +0.6
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