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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
gabT and gabP are separated by 237 nucleotides
gabP and ygaE are separated by 20 nucleotides
ygaE and ygaU are separated by 0 nucleotides
ygaU and yqaE are separated by 83 nucleotides
b2662: gabT - 4-aminobutyrate aminotransferase (NCBI), at 2,790,757 to 2,792,037
gabT
b2663: gabP - gamma-aminobutyrate transporter (NCBI), at 2,792,275 to 2,793,675
gabP
b2664: ygaE - putative transcriptional regulator (VIMSS), at 2,793,696 to 2,794,358
ygaE
b2665: ygaU - hypothetical protein (NCBI), at 2,794,359 to 2,794,808
ygaU
b2666: yqaE - predicted membrane protein (NCBI), at 2,794,892 to 2,795,050
yqaE
Group
Condition
b2662
b2663
b2664
b2665
b2666
gabT
gabP
ygaE
ygaU
yqaE
phage
T7_phage 0.0015625 MOI TC2
-0.5
-0.2
-0.6
-0.6
-1.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-0.7
-0.2
-1.0
-0.9
stress
LB Aerobic with Novobiocin 0.0000625 mM
-0.4
-0.1
+0.6
-1.1
-1.1
phage
T7_phage 0.00015625 MOI TC4
-0.6
-0.1
-0.7
+0.5
-1.0
phage
T7_phage 0.0015625 MOI TC1
-0.3
-0.2
-0.7
+0.3
-0.8
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.4
-0.0
+0.5
-0.1
-1.4
motility
outer cut, LB soft agar motility assay
+0.1
+0.1
+0.7
-0.7
-1.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
-0.2
+0.8
+0.3
-1.2
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.2
+0.2
+0.5
+0.4
-1.4
control
PBS_cage_1_mice _Feces_16 hr
+1.1
+0.1
-0.6
-0.3
-0.1
control
PBS_cage_1_mice _Feces_22 hr
+1.1
+0.2
-0.7
+0.1
-0.1
nitrogen source
L-Aspartic Acid (N)
-0.1
+0.2
+0.7
+0.4
-0.8
stress
Chloramphenicol 0.005 mg/ml
-0.3
+0.0
+1.2
+0.3
-0.5
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
+0.3
+0.0
+1.0
+0.2
-0.6
stress
LB Anaerobic with Doxycycline hyclate 0.00000078125 mM
-0.3
+0.1
+1.0
+0.3
-0.3
nitrogen source
Putrescine (N)
-0.4
-0.1
+0.6
+0.6
+0.3
nitrogen source
Putrescine (N)
-0.6
-0.2
+0.7
+0.7
+0.5
stress
Doxycycline hyclate 0.001 mg/ml
-0.2
+0.2
+1.6
-0.1
-0.3
carbon source
carbon source D-Mannose 0.5mM
+0.8
+0.0
+0.3
-0.4
+0.7
nitrogen source
L-Arginine (N)
-0.3
-0.0
+0.3
+1.3
+0.2
carbon source
Glycolic Acid (C)
+0.2
+0.3
+0.5
-0.0
+1.0
carbon source
Glycolic Acid (C)
-0.1
+0.3
+0.5
+0.2
+1.2
carbon source
succinate (C)
+0.2
+0.0
+0.1
+1.0
+0.8
carbon source
acetate (C)
+0.3
-0.1
+0.6
+0.8
+0.6
carbon source
succinate (C)
+0.2
+0.1
+0.3
+0.9
+0.9
carbon source
a-Ketoglutaric (C)
+0.2
+0.0
+0.6
+1.1
+0.6
phage
N4_phage 0.009375 MOI TC2
+0.3
+0.5
+0.5
+0.8
+0.4
carbon source
a-Ketoglutaric (C)
+0.3
+0.1
+0.3
+1.0
+0.9
carbon source
Ying_Others16 rep A; time point 2
-0.2
-0.0
+3.5
+0.3
+0.5
carbon source
Ying_Others16 rep B; time point 2
+0.1
+0.0
+3.3
+0.6
+0.5
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