Fitness for 5 genes in Escherichia coli BW25113

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500 ntgabT and gabP are separated by 237 nucleotidesgabP and ygaE are separated by 20 nucleotidesygaE and ygaU are separated by 0 nucleotidesygaU and yqaE are separated by 83 nucleotides b2662: gabT - 4-aminobutyrate aminotransferase (NCBI), at 2,790,757 to 2,792,037 gabT b2663: gabP - gamma-aminobutyrate transporter (NCBI), at 2,792,275 to 2,793,675 gabP b2664: ygaE - putative transcriptional regulator (VIMSS), at 2,793,696 to 2,794,358 ygaE b2665: ygaU - hypothetical protein (NCBI), at 2,794,359 to 2,794,808 ygaU b2666: yqaE - predicted membrane protein (NCBI), at 2,794,892 to 2,795,050 yqaE
Group Condition b2662 b2663 b2664 b2665 b2666
gabT gabP ygaE ygaU yqaE
phage T7_phage 0.0015625 MOI TC2 -0.5 -0.2 -0.6 -0.6 -1.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -0.7 -0.2 -1.0 -0.9
stress LB Aerobic with Novobiocin 0.0000625 mM -0.4 -0.1 +0.6 -1.1 -1.1
phage T7_phage 0.00015625 MOI TC4 -0.6 -0.1 -0.7 +0.5 -1.0
phage T7_phage 0.0015625 MOI TC1 -0.3 -0.2 -0.7 +0.3 -0.8
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.4 -0.0 +0.5 -0.1 -1.4
motility outer cut, LB soft agar motility assay +0.1 +0.1 +0.7 -0.7 -1.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 -0.2 +0.8 +0.3 -1.2
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 +0.2 +0.5 +0.4 -1.4
control PBS_cage_1_mice _Feces_16 hr +1.1 +0.1 -0.6 -0.3 -0.1
control PBS_cage_1_mice _Feces_22 hr +1.1 +0.2 -0.7 +0.1 -0.1
nitrogen source L-Aspartic Acid (N) -0.1 +0.2 +0.7 +0.4 -0.8
stress Chloramphenicol 0.005 mg/ml -0.3 +0.0 +1.2 +0.3 -0.5
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +0.3 +0.0 +1.0 +0.2 -0.6
stress LB Anaerobic with Doxycycline hyclate 0.00000078125 mM -0.3 +0.1 +1.0 +0.3 -0.3
nitrogen source Putrescine (N) -0.4 -0.1 +0.6 +0.6 +0.3
nitrogen source Putrescine (N) -0.6 -0.2 +0.7 +0.7 +0.5
stress Doxycycline hyclate 0.001 mg/ml -0.2 +0.2 +1.6 -0.1 -0.3
carbon source carbon source D-Mannose 0.5mM +0.8 +0.0 +0.3 -0.4 +0.7
nitrogen source L-Arginine (N) -0.3 -0.0 +0.3 +1.3 +0.2
carbon source Glycolic Acid (C) +0.2 +0.3 +0.5 -0.0 +1.0
carbon source Glycolic Acid (C) -0.1 +0.3 +0.5 +0.2 +1.2
carbon source succinate (C) +0.2 +0.0 +0.1 +1.0 +0.8
carbon source acetate (C) +0.3 -0.1 +0.6 +0.8 +0.6
carbon source succinate (C) +0.2 +0.1 +0.3 +0.9 +0.9
carbon source a-Ketoglutaric (C) +0.2 +0.0 +0.6 +1.1 +0.6
phage N4_phage 0.009375 MOI TC2 +0.3 +0.5 +0.5 +0.8 +0.4
carbon source a-Ketoglutaric (C) +0.3 +0.1 +0.3 +1.0 +0.9
carbon source Ying_Others16 rep A; time point 2 -0.2 -0.0 +3.5 +0.3 +0.5
carbon source Ying_Others16 rep B; time point 2 +0.1 +0.0 +3.3 +0.6 +0.5
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