Fitness for 5 genes in Escherichia coli BW25113

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500 ntyfjA and rpsP are separated by 18 nucleotidesrpsP and ffh are separated by 248 nucleotidesffh and ypjD are separated by 166 nucleotidesypjD and yfjD are separated by 20 nucleotides b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940 yfjA b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207 rpsP b2610: ffh - Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) (NCBI), at 2,744,456 to 2,745,817 ffh b2611: ypjD - predicted inner membrane protein (RefSeq), at 2,745,984 to 2,746,775 ypjD b4461: yfjD - predicted inner membrane protein (RefSeq), at 2,746,796 to 2,748,082 yfjD
Group Condition b2608 b2609 b2610 b2611 b4461
yfjA rpsP ffh ypjD yfjD
stress M9 with Methotrexate 0.06 mM 48 hr N.D. N.D. N.D. -1.7 -0.1
no stress control M9 no stress control; Aerobic 48 hr N.D. N.D. N.D. -1.6 -0.0
stress M9 with Methotrexate 0.06 mM 48 hr N.D. N.D. N.D. -1.5 -0.0
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin N.D. N.D. N.D. -1.6 +0.1
no stress control M9 no stress control; Aerobic 48 hr N.D. N.D. N.D. -1.5 +0.0
no stress control M9 no stress control; Aerobic 24 hr N.D. N.D. N.D. -1.5 +0.0
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin N.D. N.D. N.D. -1.5 +0.1
nutrient M9 Glucose 0.2%, Thauer's vit mix without biotin N.D. N.D. N.D. -1.5 +0.0
nutrient M9 Glucose 0.2%, Thauer's vit mix N.D. N.D. N.D. -1.6 +0.2
M9 Acetate 0.5% aerobic N.D. N.D. N.D. -1.7 +0.3
nutrient M9 Glucose 0.2%, Thauer's vit mix N.D. N.D. N.D. -1.4 +0.1
M9 Acetate 0.5% aerobic N.D. N.D. N.D. -1.6 +0.3
carbon source acetate (C) N.D. N.D. N.D. -1.7 +0.5
carbon source D-Xylose (C) N.D. N.D. N.D. -1.3 +0.2
carbon source D-Maltose (C) N.D. N.D. N.D. -1.4 +0.3
carbon source acetate (C) N.D. N.D. N.D. -1.3 +0.3
carbon source L-Malic (C) N.D. N.D. N.D. -1.2 +0.3
carbon source D-Xylose (C) N.D. N.D. N.D. -1.2 +0.4
carbon source succinate (C) N.D. N.D. N.D. -1.1 +0.4
carbon source D-Serine (C) N.D. N.D. N.D. -1.1 +0.4
carbon source D-Serine (C) N.D. N.D. N.D. -1.1 +0.4
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. N.D. -1.0 +0.5
phage HSDP4 N.D. N.D. N.D. +0.6 -1.0
stress LB Anaerobic with Furaltadone 0.000015625 mM N.D. N.D. N.D. -0.9 +0.9
carbon source Glycolic Acid (C) N.D. N.D. N.D. -0.8 +0.9
stress 2-nitrobenzenesulfonyl fluoride N.D. N.D. N.D. -0.5 +1.0
stress Nickel (II) chloride 1.5 mM N.D. N.D. N.D. +0.2 +1.4
stress Nickel (II) chloride 1.5 mM N.D. N.D. N.D. +0.1 +1.5
stress Nickel (II) chloride 1.5 mM N.D. N.D. N.D. +0.1 +2.0
stress Nickel (II) chloride 1 mM N.D. N.D. N.D. +0.6 +2.7
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