Fitness for 5 genes in Escherichia coli BW25113

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500 ntyfiB and rplS are separated by 75 nucleotidesrplS and trmD are separated by 41 nucleotidestrmD and yfjA are separated by 30 nucleotidesyfjA and rpsP are separated by 18 nucleotides b2605: yfiB - predicted outer membrane lipoprotein (NCBI), at 2,741,647 to 2,742,129 yfiB b2606: rplS - 50S ribosomal protein L19 (NCBI), at 2,742,205 to 2,742,552 rplS b2607: trmD - tRNA (guanine-N(1)-)-methyltransferase (NCBI), at 2,742,594 to 2,743,361 trmD b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940 yfjA b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207 rpsP
Group Condition b2605 b2606 b2607 b2608 b2609
yfiB rplS trmD yfjA rpsP
phage N4_phage 0.0009375 MOI TC3 -1.0 N.D. N.D. N.D. N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.9 N.D. N.D. N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.9 N.D. N.D. N.D. N.D.
nitrogen source L-Serine (N) -0.6 N.D. N.D. N.D. N.D.
stress capsaicin 1 mM -0.6 N.D. N.D. N.D. N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.6 N.D. N.D. N.D. N.D.
phage N4_phage 0.009375 MOI TC1 -0.6 N.D. N.D. N.D. N.D.
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.5 N.D. N.D. N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.5 N.D. N.D. N.D. N.D.
stress Spectinomycin 0.00625 mg/ml -0.5 N.D. N.D. N.D. N.D.
phage RB68 -0.5 N.D. N.D. N.D. N.D.
phage N4_phage 0.0009375 MOI TC2 -0.5 N.D. N.D. N.D. N.D.
phage Phage_N4_cage_3_mice _Feces_22 hr +0.6 N.D. N.D. N.D. N.D.
stress 3-methyl-3-butenol 0.625 vol% +0.6 N.D. N.D. N.D. N.D.
stress undecene 2.5 vol% +0.6 N.D. N.D. N.D. N.D.
stress Cisplatin 0.1 mg/ml +0.6 N.D. N.D. N.D. N.D.
phage Phage_N4_cage_3_mouse_2_Cecum_40 hr +0.6 N.D. N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay +0.6 N.D. N.D. N.D. N.D.
phage Phage_N4_cage_3_mice _Feces_40 hr +0.6 N.D. N.D. N.D. N.D.
phage T7_phage 0.0000015625 MOI TC2 +0.6 N.D. N.D. N.D. N.D.
stress 3-methyl-2-butenol 1.25 vol% +0.6 N.D. N.D. N.D. N.D.
stress octanol 0.08 vol% +0.6 N.D. N.D. N.D. N.D.
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.6 N.D. N.D. N.D. N.D.
stress 3-methyl-1-butanol 2.5 vol% +0.7 N.D. N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay +0.7 N.D. N.D. N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.7 N.D. N.D. N.D. N.D.
stress limonene 1.25 vol% +0.7 N.D. N.D. N.D. N.D.
carbon source carbon source D-Glucose 0.25mM +0.8 N.D. N.D. N.D. N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.9 N.D. N.D. N.D. N.D.
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +1.0 N.D. N.D. N.D. N.D.
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