Fitness for 5 genes in Escherichia coli BW25113

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500 ntaroF and yfiL are separated by 209 nucleotidesyfiL and yfiR are separated by 149 nucleotidesyfiR and yfiN overlap by 11 nucleotidesyfiN and yfiB are separated by 15 nucleotides b2601: aroF - 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, tyrosine-repressible (NCBI), at 2,738,102 to 2,739,172 aroF b2602: yfiL - orf, hypothetical protein (VIMSS), at 2,739,382 to 2,739,747 yfiL b2603: yfiR - hypothetical protein (NCBI), at 2,739,897 to 2,740,415 yfiR b2604: yfiN - predicted diguanylate cyclase (NCBI), at 2,740,405 to 2,741,631 yfiN b2605: yfiB - predicted outer membrane lipoprotein (NCBI), at 2,741,647 to 2,742,129 yfiB
Group Condition b2601 b2602 b2603 b2604 b2605
aroF yfiL yfiR yfiN yfiB
carbon source Ying_sugar7 rep C; time point 3 -2.0 +0.1 -6.5 +0.2 +0.2
carbon source Ying_sugar7 rep A; time point 3 -1.7 -0.1 -6.1 -0.0 +0.2
carbon source Ying_sugar7 rep B; time point 3 -2.2 +0.2 -5.1 -0.0 +0.2
carbon source Ying_sugar7 rep C; time point 2 -1.5 +0.1 -4.1 -0.1 +0.1
carbon source Ying_sugar7 rep A; time point 2 -1.6 -0.1 -3.6 +0.1 +0.1
carbon source Ying_sugar7 rep B; time point 2 -1.3 +0.1 -3.5 -0.0 -0.0
carbon source Ying_sugar7 rep A; time point 1 -1.0 +0.0 -2.9 -0.1 +0.4
carbon source Ying_Nucleobase14 rep B; time point 3 -0.7 -0.1 -2.6 -0.0 -0.0
carbon source Ying_sugar7 rep C; time point 1 -0.9 -0.0 -2.5 +0.1 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -1.0 -1.1 -0.2 -0.4
carbon source Ying_Nucleobase14 rep A; time point 3 -0.7 -0.1 -2.2 -0.0 +0.1
carbon source Ying_sugar7 rep B; time point 1 -0.6 +0.2 -2.6 +0.1 +0.1
carbon source Ying_Nucleobase14 rep C; time point 3 -0.8 -0.1 -2.0 +0.1 +0.4
carbon source Ying_OrganicAcid7 rep C; time point 3 -1.1 +0.2 -2.0 +0.2 +0.1
carbon source Ying_OrganicAcid7 rep A; time point 3 -1.0 +0.1 -1.8 +0.1 +0.2
carbon source Ying_Nucleobase14 rep B; time point 2 -0.6 +0.2 -2.2 +0.1 +0.2
phage R2_N4_T1_phage10 +0.1 -0.5 -2.3 +0.2 +0.2
carbon source Ying_OrganicAcid7 rep C; time point 2 -0.9 +0.2 -1.7 +0.1 -0.1
carbon source D-Lactate (C) -2.6 +0.3 -0.1 -0.0 +0.2
carbon source carbon source D-Mannose 0.5mM -0.4 -0.5 -1.7 +0.3 +0.3
carbon source Acetate (C) -2.1 +0.3 -0.2 -0.1 +0.0
carbon source Ying_Nucleobase14 rep C; time point 2 -0.6 +0.0 -1.7 +0.2 +0.2
carbon source carbon source D-Glucose 5mM -1.7 -0.4 +0.4 +0.4 -0.4
carbon source carbon source D-Glucose 0.25mM -0.8 -0.7 -1.0 -0.1 +0.8
carbon source Ying_OrganicAcid7 rep B; time point 2 -1.0 +0.3 -1.3 +0.2 +0.2
carbon source acetate (C) -2.1 +0.4 -0.1 -0.2 +0.4
carbon source acetate (C) -2.2 +0.7 -0.4 -0.0 +0.4
phage R2_N4_T2_phage10 -0.3 +0.1 -1.4 +0.5 +0.3
motility outer cut, LB soft agar motility assay -0.2 -0.3 -1.1 +0.6 +0.5
phage R1_N4_T2_phage8 +0.4 -0.5 -1.1 +0.6 +0.4
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