Fitness for 5 genes in Escherichia coli BW25113

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500 ntclpB and ryfD overlap by 21 nucleotidesryfD and yfiH are separated by 7 nucleotidesyfiH and rluD overlap by 4 nucleotidesrluD and yfiO are separated by 134 nucleotides b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317 ryfD b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056 yfiH b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD b2595: yfiO - predicted lipoprotein (NCBI), at 2,734,168 to 2,734,905 yfiO
Group Condition b2592 b4609 b2593 b2594 b2595
clpB ryfD yfiH rluD yfiO
stress Trimethoprim 0.00125 mM -0.2 -1.5 -1.2 N.D. N.D.
stress Aluminum chloride 5 mM -1.0 +0.0 -1.9 N.D. N.D.
phage N4_phage 0.09375 MOI TC1 -1.0 -0.5 -0.6 N.D. N.D.
stress Cephalothin 0.01 mg/ml -0.3 +0.1 -2.0 N.D. N.D.
phage N4_phage 0.0009375 MOI TC2 -0.7 -0.9 -0.4 N.D. N.D.
stress 3-methyl-1-butanol 1.25 vol% -0.6 -0.3 -1.0 N.D. N.D.
phage N4_phage 0.0009375 MOI TC3 -0.5 -1.2 -0.2 N.D. N.D.
phage Δdbn_10 +0.3 -1.3 -0.9 N.D. N.D.
phage N4_phage 0.00009375 MOI TC4 -0.5 -0.9 -0.5 N.D. N.D.
stress 3-methyl-1-butanol 1.25 vol% -0.4 -0.8 -0.6 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.6 -1.2 N.D. N.D.
mzaabcde mzaABCDE -0.2 -1.3 -0.2 N.D. N.D.
phage Ste41 -0.3 -1.5 +0.3 N.D. N.D.
stress Cisplatin 0.05 mg/ml +0.1 -0.8 -0.8 N.D. N.D.
stress Carbenicillin 0.004 mg/ml +0.1 +0.3 -1.7 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +0.6 -1.3 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.3 -1.2 N.D. N.D.
stress Cisplatin 0.1 mg/ml +0.1 +0.6 -1.1 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 +0.4 -1.1 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.6 -0.8 N.D. N.D.
carbon source Glycolic Acid (C) -0.4 +0.9 -0.5 N.D. N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +0.9 -0.7 N.D. N.D.
stress Doxycycline hyclate 0.001 mg/ml -0.4 -0.6 +1.1 N.D. N.D.
stress Trimethoprim 0.005 mM -0.2 +1.3 -0.4 N.D. N.D.
phage T7_phage 0.00015625 MOI TC3 -0.0 -0.7 +1.4 N.D. N.D.
stress limonene 5 vol% +0.9 +0.7 -0.4 N.D. N.D.
stress octanol 0.08 vol% +0.2 -0.5 +1.8 N.D. N.D.
stress limonene 5 vol% +1.1 +0.6 -0.0 N.D. N.D.
stress Chloramphenicol 0.001 mg/ml +0.1 +0.5 +1.1 N.D. N.D.
stress Bacitracin 1 mg/ml +0.2 +0.6 +1.9 N.D. N.D.
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