Fitness for 5 genes in Escherichia coli BW25113

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500 ntpssA and yfiM are separated by 45 nucleotidesyfiM and kgtP overlap by 4 nucleotideskgtP and rrfG are separated by 322 nucleotidesrrfG and rrlG are separated by 92 nucleotides b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104 pssA b2586: yfiM - predicted protein (RefSeq), at 2,722,150 to 2,722,473 yfiM b2587: kgtP - alpha-ketoglutarate transporter (NCBI), at 2,722,470 to 2,723,768 kgtP b2588: rrfG - 5S ribosomal RNA (NCBI), at 2,724,091 to 2,724,210 rrfG b2589: rrlG - 23S ribosomal RNA (NCBI), at 2,724,303 to 2,727,206 rrlG
Group Condition b2585 b2586 b2587 b2588 b2589
pssA yfiM kgtP rrfG rrlG
carbon source a-Ketoglutaric (C) N.D. -1.0 -5.5 N.D. N.D.
carbon source a-Ketoglutaric (C) N.D. -0.7 -4.6 N.D. N.D.
carbon source Glycolic Acid (C) N.D. -0.6 -3.0 N.D. N.D.
carbon source Glycolic Acid (C) N.D. -0.2 -3.3 N.D. N.D.
carbon source succinate (C) N.D. -0.2 -3.3 N.D. N.D.
carbon source succinate (C) N.D. -0.3 -3.0 N.D. N.D.
carbon source L-Malic (C) N.D. -0.4 -2.8 N.D. N.D.
carbon source L-Malic (C) N.D. -0.4 -2.1 N.D. N.D.
phage T7_phage 0.0015625 MOI TC2 N.D. -1.6 -0.2 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -2.0 +0.5 N.D. N.D.
phage R2_N4_T2_phage10 N.D. -1.5 +0.3 N.D. N.D.
balo Mixed culture, Halobacteriovorax-10 N.D. -1.4 +0.4 N.D. N.D.
phage RB68 N.D. -1.2 +0.4 N.D. N.D.
phage Δdbn_10 N.D. -1.3 +0.7 N.D. N.D.
stress Benzalkonium Chloride 0.004 mg/ml N.D. -1.3 +1.4 N.D. N.D.
stress Cephalothin 0.01 mg/ml N.D. -0.7 +1.3 N.D. N.D.
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) N.D. -0.3 +1.3 N.D. N.D.
plasmid transformation vcDART vector transformation; agar assay N.D. +0.6 +0.9 N.D. N.D.
plasmid transformation vcDART vector transformation; agar assay N.D. +0.4 +1.2 N.D. N.D.
stress Carbenicillin 0.008 mg/ml N.D. +0.5 +1.1 N.D. N.D.
carbon source D-Serine (C) N.D. -0.6 +2.2 N.D. N.D.
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) N.D. +0.5 +1.2 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.5 +1.1 N.D. N.D.
predation 48 hr rep2_HD100_no_dilution N.D. +0.3 +1.4 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.5 +1.2 N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. +1.1 +0.8 N.D. N.D.
plasmid transformation vcDART vector transformation; agar assay N.D. +0.7 +1.2 N.D. N.D.
carbon source D-Serine (C) N.D. +0.0 +2.2 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.2 +2.5 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.1 +2.1 N.D. N.D.
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