Fitness for 5 genes in Escherichia coli BW25113

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500 ntlepB and lepA are separated by 15 nucleotideslepA and rseC are separated by 197 nucleotidesrseC and rseB overlap by 4 nucleotidesrseB and rseA overlap by 1 nucleotides b2568: lepB - leader peptidase (signal peptidase I) (NCBI), at 2,702,357 to 2,703,331 lepB b2569: lepA - GTP-binding protein LepA (NCBI), at 2,703,347 to 2,705,146 lepA b2570: rseC - RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI), at 2,705,344 to 2,705,823 rseC b2571: rseB - periplasmic negative regulator of sigmaE (NCBI), at 2,705,820 to 2,706,776 rseB b2572: rseA - anti-sigma factor (NCBI), at 2,706,776 to 2,707,426 rseA
Group Condition b2568 b2569 b2570 b2571 b2572
lepB lepA rseC rseB rseA
phage T7_phage 0.00015625 MOI TC2 N.D. -3.7 -1.9 -1.7 -2.6
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. -1.8 +0.9 -5.1 -3.7
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. -2.0 +0.6 -4.1 -3.4
phage T7_phage 0.0015625 MOI TC1 N.D. -2.9 -2.1 -1.1 -2.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM N.D. +0.0 +0.5 -4.3 -4.7
phage phage N.D. -1.2 -0.7 -2.7 -2.4
phage T7_phage 0.0015625 MOI TC2 N.D. -3.3 -1.5 -0.6 -1.4
phage T7_phage 0.000015625 MOI TC3 N.D. -2.4 -0.8 -0.9 -2.2
phage T7_phage 0.00015625 MOI TC4 N.D. -1.6 -1.0 -1.7 -1.7
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM N.D. +0.1 -0.7 -3.7 -1.6
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM N.D. -1.2 +0.4 -3.0 -2.0
stress 2,4-Dinitrophenol 2 mM N.D. -1.1 -0.5 -1.7 -2.5
stress Bacitracin 1 mg/ml N.D. -0.3 +0.9 -3.5 -2.9
stress LB Anaerobic with Benzethonium chloride 0.000015625 mM N.D. -0.8 -0.1 -3.3 -1.5
stress BHIS with Methotrexate 1.98 mM 48 hr N.D. -0.9 +0.4 -3.8 -1.3
stress Chloride 750 mM N.D. -0.7 -0.5 -3.7 -0.7
stress BHIS with Methotrexate 0.1 mM 48 hr N.D. -1.6 -0.1 -2.7 -1.0
stress BHIS with Methotrexate 1.98 mM 48 hr N.D. -0.8 +0.4 -3.4 -1.0
stress BHIS with Methotrexate 1.98 mM 48 hr N.D. -0.6 +0.7 -3.5 -1.3
stress Nalidixic 0.06 mM N.D. +0.0 +1.4 -1.8 -2.4
stress octanol 0.08 vol% N.D. -1.7 +3.2 -1.2 -2.9
stress Chloramphenicol 0.001 mg/ml N.D. -1.7 +1.9 -1.3 -1.4
stress Nalidixic 0.06 mM N.D. +0.3 +1.5 -1.8 -2.1
stress Nalidixic 0.006 mg/ml N.D. +0.8 +1.1 -1.8 -2.2
stress Doxycycline hyclate 0.001 mg/ml N.D. +0.4 +1.4 -2.0 -1.4
stress Benzalkonium Chloride 0.004 mg/ml N.D. +2.3 +4.4 -3.3 -2.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.8 +1.4 +2.5 +2.2
stress LB Anaerobic with Norfloxacin 0.00000015625 mM N.D. +0.6 +0.9 +1.4 +2.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.5 +0.8 +3.7 +3.8
stress Cephalothin 0.01 mg/ml N.D. +1.0 +1.2 +3.6 +4.2
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