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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yfhB and yfhH are separated by 208 nucleotides
yfhH and yfhL are separated by 55 nucleotides
yfhL and shoB are separated by 193 nucleotides
shoB and ryfC are separated by 322 nucleotides
b2560: yfhB - orf, hypothetical protein (VIMSS), at 2,695,937 to 2,696,572
yfhB
b2561: yfhH - orf, hypothetical protein (VIMSS), at 2,696,781 to 2,697,629
yfhH
b2562: yfhL - predicted 4Fe-4S cluster-containing protein (NCBI), at 2,697,685 to 2,697,945
yfhL
b4687: shoB - toxic membrane protein (RefSeq), at 2,698,139 to 2,698,219
shoB
b4608: ryfC - Novel sRNA, function unknown (RefSeq), at 2,698,542 to 2,698,618
ryfC
Group
Condition
b2560
b2561
b2562
b4687
b4608
yfhB
yfhH
yfhL
shoB
ryfC
stress
LB Aerobic with Oxytetracycline 0.00000125 mM
-0.4
+0.2
-0.2
-0.2
-2.4
phage
Ste41
-0.8
-0.7
-0.2
+0.1
-1.3
stress
Ciprofloxacin Hydrochloride 0.0024 mM
-0.3
-0.4
-0.1
-0.8
-1.1
stress
Tetracycline 0.0008 mg/ml
-0.2
-0.2
+0.1
-0.3
-2.1
stress
Aluminum chloride 5 mM
-0.3
-0.1
-0.4
-1.2
-0.5
stress
Spectinomycin 0.0125 mg/ml
-0.2
-0.1
+0.1
-0.2
-2.1
stress
LB Aerobic with Tetracycline 0.00000125 mM
-0.2
+0.1
+0.1
-0.0
-2.4
stress
Chloramphenicol 0.001 mg/ml
-0.1
-0.1
+0.1
-0.1
-2.1
motility
inner cut, LB soft agar motility assay
+0.4
+0.3
+0.1
-0.1
-2.9
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.2
+0.2
+0.1
+0.3
-2.9
phage
T7_phage 0.00015625 MOI TC4
-0.7
+0.0
-0.2
+0.1
-1.3
stress
Doxycycline hyclate 0.001 mg/ml
-0.1
-0.3
+0.2
+0.0
-1.9
stress
LB Aerobic with Spiramycin 0.0000625 mM
-0.0
+0.2
-0.0
+0.2
-2.2
stress
LB Aerobic with Spiramycin 0.000125 mM
+0.0
+0.2
-0.1
-0.1
-1.9
stress
LB Aerobic with Oxytetracycline 0.00000125 mM
-0.0
+0.2
-0.4
+0.3
-2.0
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
-0.1
-0.0
+0.2
+0.3
-2.2
stress
Spectinomycin 0.00625 mg/ml
-0.3
-0.3
-0.3
+0.3
-1.1
stress
LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM
-0.3
+0.1
+0.4
-0.3
-1.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
+0.0
-0.3
+0.4
-1.9
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.1
-0.7
+0.4
+0.5
-1.3
carbon source
carbon source D-Mannose 0.5mM
+0.3
-0.8
+0.3
-1.1
+0.1
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
-0.2
-0.1
+0.1
+0.7
-1.7
phage
Ste41
-0.6
-0.1
+0.0
+0.8
-0.9
stress
Benzalkonium Chloride 0.004 mg/ml
-0.3
-0.4
+0.7
-1.0
+0.4
control
PBS_cage_2_mouse_3_Cecum_40 hr
+0.4
+0.1
-1.0
+0.3
-0.3
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.3
-0.2
-1.2
+0.4
+0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.4
+0.4
-0.0
-1.2
+0.7
control
PBS_cage_1_mice _Feces_22 hr
-0.1
+0.3
-0.6
+1.1
-0.4
control
PBS_cage_2_mouse_1_Cecum_40 hr
+0.8
-0.1
-0.6
+0.3
+0.7
phage
T7_phage 0.0015625 MOI TC2
-0.0
-0.5
+0.4
+0.6
+1.2
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