Fitness for 5 genes in Escherichia coli BW25113

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500 nthmp and glnB are separated by 44 nucleotidesglnB and yfhA are separated by 60 nucleotidesyfhA and yfhG overlap by 11 nucleotidesyfhG and yfhK are separated by 164 nucleotides b2552: hmp - fused nitric oxide dioxygenase/dihydropteridine reductase 2 (NCBI), at 2,683,857 to 2,685,047 hmp b2553: glnB - regulatory protein P-II for glutamine synthetase (NCBI), at 2,685,092 to 2,685,430 glnB b2554: yfhA - predicted DNA-binding response regulator in two-component system (NCBI), at 2,685,491 to 2,686,825 yfhA b2555: yfhG - hypothetical protein (NCBI), at 2,686,815 to 2,687,528 yfhG b2556: yfhK - putative 2-component sensor protein (VIMSS), at 2,687,693 to 2,689,120 yfhK
Group Condition b2552 b2553 b2554 b2555 b2556
hmp glnB yfhA yfhG yfhK
stress LB Aerobic with Irgasan 0.0000003125 mM +0.1 -1.6 -1.7 -1.0 -1.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.8 -4.5 -0.2 +0.1 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 -4.0 -0.4 -0.4 -0.4
stress Cephalothin 0.01 mg/ml -0.0 -3.2 -0.9 -0.6 -0.4
stress undecene 2.5 vol% +0.0 -1.3 -1.3 -1.4 -0.9
stress undecene 2.5 vol% -0.1 -1.1 -1.3 -1.2 -1.0
stress undecene 5 vol% -0.0 -0.9 -1.5 -1.2 -0.9
stress Benzalkonium Chloride 0.004 mg/ml -0.3 -3.1 -0.4 -0.2 -0.4
stress undecene 5 vol% +0.0 -0.8 -1.5 -1.2 -0.9
stress octanol 0.08 vol% +0.6 -3.3 -0.2 -1.3 +0.1
stress 2,4-Dinitrophenol 2 mM +0.0 -2.0 -0.6 -0.6 -0.3
stress Carbenicillin 0.008 mg/ml -0.1 -2.1 -0.5 -0.5 -0.3
stress 2,4-Dinitrophenol 2 mM -0.2 -1.3 -0.5 -0.6 -0.4
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.1 -3.0 -0.1 +0.1 +0.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -2.2 -0.1 +0.0 +0.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.0 -2.8 +0.3 -0.1 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.2 -3.3 +0.3 +0.6 +0.2
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 -2.2 +0.1 +0.3 +0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.3 -3.0 +0.5 +0.4 +0.2
stress LB Anaerobic with Ticarcillin 0.00003125 mM -0.3 -1.9 +0.3 +0.3 +0.0
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.1 -2.8 +0.5 +0.6 +0.4
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.4 -2.9 +0.6 +0.7 +0.2
stress Cisplatin 0.1 mg/ml +0.2 -1.2 -0.2 +1.0 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 -0.9 +0.7 +0.7 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -0.4 +0.7 +0.9 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.4 +0.7 +0.6 +0.6 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.2 +0.9 +0.9 +0.7
plasmid transformation vcDART vector transformation; agar assay +0.4 +1.0 +0.7 +0.5 +0.5
plasmid transformation vcDART vector transformation; agar assay +0.3 +1.2 +0.6 +0.6 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.9 +0.8 +1.1 +0.9
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