Fitness for 5 genes in Escherichia coli BW25113

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500 nthcaF and hcaC overlap by 1 nucleotideshcaC and hcaB overlap by 4 nucleotideshcaB and hcaD are separated by 9 nucleotideshcaD and yphA are separated by 96 nucleotides b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930 hcaF b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250 hcaC b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059 hcaB b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271 hcaD b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790 yphA
Group Condition b2539 b2540 b2541 b2542 b2543
hcaF hcaC hcaB hcaD yphA
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.8 -0.2 +0.1 -1.2 -0.2
phage RB51m1 -0.2 -0.9 -0.7 -0.1 -0.2
phage RB51m1 -1.4 -0.5 +0.2 +0.3 -0.5
msc 22hr_MSC_rep1_highinoculum +0.0 -1.7 -0.3 +0.3 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.2 -0.5 -0.1 -0.6 -0.8
stress octanol 0.08 vol% -0.3 -0.6 -0.9 +0.1 -0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -1.5 +0.6 -0.3 -0.4 -0.1
motility outer cut, LB soft agar motility assay -0.8 -0.9 +0.1 +0.2 -0.2
phage Δdbn_10 +0.6 -0.5 -0.3 -0.5 -0.6
motility outer cut, LB soft agar motility assay +0.2 -1.6 +0.5 +0.1 -0.4
phage T7_phage 0.0015625 MOI TC2 -0.2 -0.9 +0.5 -0.4 +0.3
carbon source carbon source D-Mannose 0.5mM +0.7 -0.3 +0.1 +0.1 -1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -1.2 +0.1 +0.7 -0.2
stress Benzalkonium Chloride 0.004 mg/ml +0.7 -0.4 +0.2 -0.4 -0.6
phage RhdML5 +0.5 -1.0 +0.4 -0.7 +0.4
mixed community control; abiotic network mixed community control; abiotic network +0.2 +0.4 -0.8 +0.3 -0.4
carbon source carbon source D-Glucose 0.25mM +0.5 -0.6 -0.7 +0.3 +0.4
no phage control CTR +0.0 -1.2 +0.5 +0.6 -0.1
no phage control NophageCTRA12 -0.0 -1.0 +0.5 +0.6 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.9 +0.0 -0.1 -0.2 -1.5
stress Bacitracin 1 mg/ml -0.2 +0.7 +0.6 -0.4 -0.2
motility inner cut, LB soft agar motility assay +0.5 +0.4 +0.5 -0.5 -0.4
carbon source carbon source D-Glucose 0.25mM +0.8 -0.2 -0.3 -0.1 +0.8
control PBS_cage_1_mice _Feces_16 hr -0.2 +0.6 -0.2 +0.6 +0.3
carbon source carbon source D-Mannose 20mM +0.6 -0.5 +0.5 -0.1 +0.5
phage Ste41 +0.8 +0.5 +0.5 -0.1 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 +0.8 +0.0 +1.1 -0.1
phage PoolT9D30 +1.3 +1.1 -0.3 +0.1 -0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +2.3 +0.3 -0.4 +0.0 -0.1
carbon source carbon source D-Glucose 20mM +0.6 +1.0 +0.3 +0.1 +0.2
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