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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
hcaF and hcaC overlap by 1 nucleotides
hcaC and hcaB overlap by 4 nucleotides
hcaB and hcaD are separated by 9 nucleotides
hcaD and yphA are separated by 96 nucleotides
b2539: hcaF - 3-phenylpropionate dioxygenase, small (beta) subunit (NCBI), at 2,668,412 to 2,668,930
hcaF
b2540: hcaC - 3-phenylpropionate dioxygenase, predicted ferredoxin subunit (NCBI), at 2,668,930 to 2,669,250
hcaC
b2541: hcaB - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase (NCBI), at 2,669,247 to 2,670,059
hcaB
b2542: hcaD - phenylpropionate dioxygenase, ferredoxin reductase subunit (NCBI), at 2,670,069 to 2,671,271
hcaD
b2543: yphA - predicted inner membrane protein (RefSeq), at 2,671,368 to 2,671,790
yphA
Group
Condition
b2539
b2540
b2541
b2542
b2543
hcaF
hcaC
hcaB
hcaD
yphA
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.8
-0.2
+0.1
-1.2
-0.2
msc
22hr_MSC_rep1_highinoculum
+0.0
-1.7
-0.3
+0.3
-0.2
carbon source
acetate (C)
+0.0
-1.5
-0.2
+0.0
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.2
-0.5
-0.1
-0.6
-0.8
stress
octanol 0.08 vol%
-0.3
-0.6
-0.9
+0.1
-0.2
stress
3-methyl-1-butanol 1.25 vol%
-0.6
-0.0
-1.0
+0.1
-0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-1.5
+0.6
-0.3
-0.4
-0.1
phage
N4_phage 0.09375 MOI TC1
+0.1
-0.8
-0.9
-0.0
-0.1
motility
outer cut, LB soft agar motility assay
-0.8
-0.9
+0.1
+0.2
-0.2
phage
Δdbn_10
+0.6
-0.5
-0.3
-0.5
-0.6
motility
outer cut, LB soft agar motility assay
+0.2
-1.6
+0.5
+0.1
-0.4
phage
T7_phage 0.0015625 MOI TC2
-0.2
-0.9
+0.5
-0.4
+0.3
carbon source
carbon source D-Mannose 0.5mM
+0.7
-0.3
+0.1
+0.1
-1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.0
-1.2
+0.1
+0.7
-0.2
stress
Benzalkonium Chloride 0.004 mg/ml
+0.7
-0.4
+0.2
-0.4
-0.6
stress
Tetracycline 0.0004 mg/ml
+0.5
+0.2
-0.6
-0.5
+0.2
carbon source
carbon source D-Glucose 0.25mM
+0.5
-0.6
-0.7
+0.3
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.9
+0.0
-0.1
-0.2
-1.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.4
-0.4
+0.6
+0.1
-0.5
motility
inner cut, LB soft agar motility assay
+0.7
-0.4
-0.0
+0.5
-0.3
stress
Bacitracin 1 mg/ml
-0.2
+0.7
+0.6
-0.4
-0.2
motility
inner cut, LB soft agar motility assay
+0.5
+0.4
+0.5
-0.5
-0.4
carbon source
carbon source D-Glucose 0.25mM
+0.8
-0.2
-0.3
-0.1
+0.8
control
PBS_cage_1_mice _Feces_16 hr
-0.2
+0.6
-0.2
+0.6
+0.3
carbon source
carbon source D-Mannose 20mM
+0.6
-0.5
+0.5
-0.1
+0.5
phage
T7_phage 0.0015625 MOI TC1
+0.0
+1.3
-0.0
-0.2
+0.4
phage
Ste41
+0.8
+0.5
+0.5
-0.1
-0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
+0.8
+0.0
+1.1
-0.1
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+2.3
+0.3
-0.4
+0.0
-0.1
carbon source
carbon source D-Glucose 20mM
+0.6
+1.0
+0.3
+0.1
+0.2
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