Fitness for 5 genes in Escherichia coli BW25113

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500 ntiscR and yfhQ are separated by 451 nucleotidesyfhQ and suhB are separated by 118 nucleotidessuhB and yfhR are separated by 144 nucleotidesyfhR and csiE are separated by 190 nucleotides b2531: iscR - DNA-binding transcriptional repressor (NCBI), at 2,659,665 to 2,660,153 iscR b2532: yfhQ - predicted methyltransferase (NCBI), at 2,660,605 to 2,661,345 yfhQ b2533: suhB - inositol monophosphatase (NCBI), at 2,661,464 to 2,662,267 suhB b2534: yfhR - predicted peptidase (RefSeq), at 2,662,412 to 2,663,266 yfhR b2535: csiE - orf, hypothetical protein (VIMSS), at 2,663,457 to 2,664,737 csiE
Group Condition b2531 b2532 b2533 b2534 b2535
iscR yfhQ suhB yfhR csiE
stress Tailocin 256 -6.7 -0.2 N.D. -0.1 -0.0
carbon source carbon source D-Mannose 1mM -5.8 -0.5 N.D. -0.1 +0.1
stress octanol 0.08 vol% -5.0 -0.5 N.D. -0.2 -0.0
phage T7_phage 0.00015625 MOI TC4 -4.5 +0.6 N.D. -0.5 -1.3
motility outer cut, LB soft agar motility assay -5.4 +0.2 N.D. -0.2 -0.2
gfp_control GFP_control -6.2 +0.4 N.D. +0.3 +0.0
phage T7_phage 0.0015625 MOI TC1 -5.2 +0.8 N.D. -0.4 -0.7
stress toluene 2.5 vol% -5.4 +0.4 N.D. -0.4 -0.0
phage Goslar -5.6 -0.3 N.D. +0.1 +0.5
control PBS_cage_2_mouse_1_Cecum_40 hr -4.4 -0.3 N.D. +0.1 -0.1
sir2-hera SIR2-HerA -4.9 -0.1 N.D. +0.2 +0.2
phage T7_phage 0.00015625 MOI TC3 -5.1 +0.3 N.D. +0.4 -0.2
phage Mnm12 -4.3 -0.0 N.D. -0.2 +0.0
stress Isobutyl alcohol 1.25 vol% -4.4 +0.0 N.D. +0.1 +0.0
phage Δdbn_10 -4.2 +0.0 N.D. -0.2 +0.1
phage T7_phage 0.00015625 MOI TC2 -4.2 +0.3 N.D. -0.0 -0.3
phage Lambda_ci1857 -3.1 -1.2 N.D. +0.2 -0.1
stress 3-methyl-1-butanol 1.25 vol% -3.5 -0.8 N.D. +0.2 +0.0
phage T7_phage 0.0015625 MOI TC2 -4.2 +0.1 N.D. -0.0 +0.5
stress Cisplatin 0.1 mg/ml -4.1 +0.3 N.D. +0.2 +0.1
phage phage -3.1 +0.7 N.D. -0.2 -0.6
phage NpK -3.3 +0.4 N.D. +0.7 +0.2
stress Trimethoprim 0.0025 mM +3.0 -1.1 N.D. +0.1 +0.3
carbon source succinate (C) +4.3 -1.0 N.D. -0.1 +0.8
carbon source succinate (C) +4.6 -0.9 N.D. -0.0 +0.8
plasmid transformation vcDART vector transformation; agar assay +3.5 +0.8 N.D. +0.4 -0.1
carbon source L-Malic (C) +4.7 -0.7 N.D. -0.1 +1.4
carbon source L-Malic (C) +4.6 -0.7 N.D. -0.1 +1.4
carbon source Glycolic Acid (C) +4.9 -0.2 N.D. -0.1 +2.6
carbon source Glycolic Acid (C) +5.1 +0.1 N.D. -0.0 +2.4
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