Fitness for 5 genes in Escherichia coli BW25113

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500 nthyfR and focB are separated by 21 nucleotidesfocB and yfgO are separated by 37 nucleotidesyfgO and yfgC are separated by 212 nucleotidesyfgC and yfgD are separated by 20 nucleotides b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934 hyfR b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804 focB b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903 yfgO b2494: yfgC - predicted peptidase (NCBI), at 2,614,116 to 2,615,579 yfgC b2495: yfgD - predicted oxidoreductase (NCBI), at 2,615,600 to 2,615,959 yfgD
Group Condition b2491 b2492 b2493 b2494 b2495
hyfR focB yfgO yfgC yfgD
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 -0.5 -0.6 -4.9 -0.6
stress LB Aerobic with Spiramycin 0.000125 mM -0.0 +0.2 +0.1 -5.9 -0.5
stress undecene 2.5 vol% +0.1 -0.2 -0.0 -5.5 -0.4
stress undecene 5 vol% +0.1 -0.1 +0.1 -5.4 -0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.1 -0.2 -1.8 -3.3 -0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 -0.5 -0.6 -4.1 -0.4
stress LB Aerobic with Spiramycin 0.0000625 mM -0.1 +0.0 +0.1 -5.5 -0.0
stress LB Aerobic with Spiramycin 0.000125 mM -0.1 +0.1 +0.1 -5.5 -0.0
stress undecene 5 vol% +0.1 -0.1 -0.3 -4.8 -0.3
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 +0.3 -0.6 -5.5 +0.3
stress Nalidixic 0.06 mM +0.0 -0.1 +0.1 -5.0 -0.1
stress undecene 2.5 vol% +0.0 -0.0 -0.1 -4.0 -0.6
stress Nalidixic 0.006 mg/ml +0.1 -0.2 -0.5 -4.0 -0.1
stress 2,4-Dinitrophenol 2 mM +0.1 -0.1 -1.5 -2.4 -0.4
stress LB Aerobic with Spiramycin 0.0000625 mM -0.0 +0.2 +0.3 -4.6 -0.1
stress LB Anaerobic with Spiramycin 0.000125 mM +0.1 +0.2 +0.2 -4.4 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.1 +0.0 -0.1 -4.0 -0.2
stress 2,4-Dinitrophenol 2 mM +0.2 -0.0 -1.8 -2.2 -0.0
stress Nalidixic 0.06 mM +0.0 +0.1 -0.0 -3.8 -0.1
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.2 +0.1 -0.3 -3.8 -0.1
stress LB Anaerobic with Spiramycin 0.000125 mM +0.0 +0.7 +0.0 -4.1 -0.5
stress Bacitracin 0.5 mg/ml -0.1 +0.4 +0.4 -4.3 -0.1
stress LB Aerobic with Novobiocin 0.0000625 mM -0.2 +0.1 +0.2 -3.2 -0.0
stress octanol 0.08 vol% -0.0 +0.2 +0.5 -3.1 -0.4
stress Bacitracin 1 mg/ml +0.1 -0.1 +1.1 -4.0 +0.0
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.5 +0.3 +0.0 -2.9 -0.3
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.1 +0.2 +0.6 -3.0 +0.0
stress Fusidic 0.03 mg/ml -0.2 +0.2 +0.3 -2.6 +0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.6 -0.2 -1.6 -1.1 +0.6
stress Butanol 1.25 vol% -0.2 +0.0 +3.3 +0.2 +0.1
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