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Cofit
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
hyfR and focB are separated by 21 nucleotides
focB and yfgO are separated by 37 nucleotides
yfgO and yfgC are separated by 212 nucleotides
yfgC and yfgD are separated by 20 nucleotides
b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934
hyfR
b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804
focB
b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903
yfgO
b2494: yfgC - predicted peptidase (NCBI), at 2,614,116 to 2,615,579
yfgC
b2495: yfgD - predicted oxidoreductase (NCBI), at 2,615,600 to 2,615,959
yfgD
Group
Condition
b2491
b2492
b2493
b2494
b2495
hyfR
focB
yfgO
yfgC
yfgD
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.1
-0.5
-0.6
-4.9
-0.6
stress
LB Aerobic with Spiramycin 0.000125 mM
-0.0
+0.2
+0.1
-5.9
-0.5
stress
undecene 2.5 vol%
+0.1
-0.2
-0.0
-5.5
-0.4
stress
undecene 5 vol%
+0.1
-0.1
+0.1
-5.4
-0.5
stress
Benzalkonium Chloride 0.004 mg/ml
+0.1
-0.2
-1.8
-3.3
-0.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
-0.5
-0.6
-4.1
-0.4
stress
LB Aerobic with Spiramycin 0.0000625 mM
-0.1
+0.0
+0.1
-5.5
-0.0
stress
LB Aerobic with Spiramycin 0.000125 mM
-0.1
+0.1
+0.1
-5.5
-0.0
stress
undecene 5 vol%
+0.1
-0.1
-0.3
-4.8
-0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.3
+0.3
-0.6
-5.5
+0.3
stress
Nalidixic 0.06 mM
+0.0
-0.1
+0.1
-5.0
-0.1
stress
undecene 2.5 vol%
+0.0
-0.0
-0.1
-4.0
-0.6
stress
Nalidixic 0.006 mg/ml
+0.1
-0.2
-0.5
-4.0
-0.1
stress
2,4-Dinitrophenol 2 mM
+0.1
-0.1
-1.5
-2.4
-0.4
stress
LB Aerobic with Spiramycin 0.0000625 mM
-0.0
+0.2
+0.3
-4.6
-0.1
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.1
+0.2
+0.2
-4.4
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.1
+0.0
-0.1
-4.0
-0.2
stress
2,4-Dinitrophenol 2 mM
+0.2
-0.0
-1.8
-2.2
-0.0
stress
Nalidixic 0.06 mM
+0.0
+0.1
-0.0
-3.8
-0.1
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.2
+0.1
-0.3
-3.8
-0.1
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.0
+0.7
+0.0
-4.1
-0.5
stress
Bacitracin 0.5 mg/ml
-0.1
+0.4
+0.4
-4.3
-0.1
stress
LB Aerobic with Novobiocin 0.0000625 mM
-0.2
+0.1
+0.2
-3.2
-0.0
stress
octanol 0.08 vol%
-0.0
+0.2
+0.5
-3.1
-0.4
stress
Bacitracin 1 mg/ml
+0.1
-0.1
+1.1
-4.0
+0.0
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.5
+0.3
+0.0
-2.9
-0.3
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.1
+0.2
+0.6
-3.0
+0.0
stress
Fusidic 0.03 mg/ml
-0.2
+0.2
+0.3
-2.6
+0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.6
-0.2
-1.6
-1.1
+0.6
stress
Butanol 1.25 vol%
-0.2
+0.0
+3.3
+0.2
+0.1
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