Fitness for 5 genes in Escherichia coli BW25113

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500 ntbcp and hyfA are separated by 252 nucleotideshyfA and hyfB overlap by 1 nucleotideshyfB and hyfC are separated by 10 nucleotideshyfC and hyfD are separated by 16 nucleotides b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970 bcp b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840 hyfA b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858 hyfB b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816 hyfC b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272 hyfD
Group Condition b2480 b2481 b2482 b2483 b2484
bcp hyfA hyfB hyfC hyfD
stress Bacitracin 1 mg/ml -1.4 -0.7 -0.1 +0.2 -0.1
stress 3-methyl-1-butanol 1.25 vol% -0.4 -0.3 -0.4 -0.8 -0.3
stress 3-methyl-1-butanol 1.25 vol% -0.8 -0.9 +0.2 -0.6 +0.1
stress Aluminum chloride 5 mM -1.1 +0.7 +0.0 -1.0 -0.3
carbon source carbon source D-Glucose 20mM -0.6 -0.8 -0.5 +0.3 +0.2
carbon source carbon source D-Mannose 0.5mM +0.5 -1.4 -0.1 +0.1 -0.4
carbon source L-Malic (C) +0.9 -0.6 -0.3 -0.1 -0.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.6 +0.5 +0.5 -0.3 +0.0
carbon source Glycolic Acid (C) +0.5 -0.9 -0.0 +0.2 +0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.6 +0.6 -0.2 -0.7 -0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.8 -0.7 +0.2 -0.0 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.8 -0.1 -0.3 +0.3 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.1 -0.0 -0.1 -0.5 -0.1
stress Cephalothin 0.01 mg/ml +0.4 +0.6 +0.3 -0.5 -0.2
phage Ste41 +0.7 +0.7 -0.3 -0.4 +0.1
control PBS_cage_1_mouse_1_Cecum_40 hr +0.7 +0.4 +0.2 -0.3 +0.0
stress LB Anaerobic with Amoxicillin 0.0000078125 mM +0.9 +0.4 +0.1 +0.2 -0.4
carbon source D-Serine (C) +1.1 +0.2 +0.1 +0.2 -0.3
phage R2_N4_T1_phage10 +0.6 +0.7 -0.1 -0.3 +0.5
stress 1-ethyl-3-methylimidazolium acetate 30 mM +0.8 +0.7 +0.1 -0.1 -0.2
stress M9 with Methotrexate 1.98 mM 24 hr +1.5 +0.1 -0.0 +0.2 -0.3
phage T7_phage 0.00015625 MOI TC4 +0.3 +1.5 -0.3 -0.1 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.5 -0.1 -0.3 +0.3 +0.2
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +1.4 +0.2 +0.3 -0.0 +0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.4 +0.2 +0.3 +0.3 -0.5
stress LB Anaerobic with Ampicillin 0.00000195313 mM +0.7 +0.1 +0.4 +0.3 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM +0.9 +0.5 +0.1 +0.2 +0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.0 -0.0 +0.5 +0.5 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.3 +1.0 +0.4 -0.3 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +1.0 +1.5 +0.0 +0.6 -0.0
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