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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
bcp and hyfA are separated by 252 nucleotides
hyfA and hyfB overlap by 1 nucleotides
hyfB and hyfC are separated by 10 nucleotides
hyfC and hyfD are separated by 16 nucleotides
b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970
bcp
b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840
hyfA
b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858
hyfB
b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816
hyfC
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
Group
Condition
b2480
b2481
b2482
b2483
b2484
bcp
hyfA
hyfB
hyfC
hyfD
stress
Bacitracin 1 mg/ml
-1.4
-0.7
-0.1
+0.2
-0.1
stress
3-methyl-1-butanol 1.25 vol%
-0.4
-0.3
-0.4
-0.8
-0.3
stress
3-methyl-1-butanol 1.25 vol%
-0.8
-0.9
+0.2
-0.6
+0.1
stress
Aluminum chloride 5 mM
-1.1
+0.7
+0.0
-1.0
-0.3
carbon source
carbon source D-Glucose 20mM
-0.6
-0.8
-0.5
+0.3
+0.2
carbon source
carbon source D-Mannose 0.5mM
+0.5
-1.4
-0.1
+0.1
-0.4
carbon source
L-Malic (C)
+0.9
-0.6
-0.3
-0.1
-0.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.6
+0.5
+0.5
-0.3
+0.0
carbon source
Glycolic Acid (C)
+0.5
-0.9
-0.0
+0.2
+0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.6
+0.6
-0.2
-0.7
-0.1
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.8
-0.7
+0.2
-0.0
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.8
-0.1
-0.3
+0.3
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.1
-0.0
-0.1
-0.5
-0.1
stress
Cephalothin 0.01 mg/ml
+0.4
+0.6
+0.3
-0.5
-0.2
phage
Ste41
+0.7
+0.7
-0.3
-0.4
+0.1
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.7
+0.4
+0.2
-0.3
+0.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.9
+0.4
+0.1
+0.2
-0.4
carbon source
D-Serine (C)
+1.1
+0.2
+0.1
+0.2
-0.3
phage
R2_N4_T1_phage10
+0.6
+0.7
-0.1
-0.3
+0.5
stress
1-ethyl-3-methylimidazolium acetate 30 mM
+0.8
+0.7
+0.1
-0.1
-0.2
stress
M9 with Methotrexate 1.98 mM 24 hr
+1.5
+0.1
-0.0
+0.2
-0.3
phage
T7_phage 0.00015625 MOI TC4
+0.3
+1.5
-0.3
-0.1
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.5
-0.1
-0.3
+0.3
+0.2
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
+1.4
+0.2
+0.3
-0.0
+0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+1.4
+0.2
+0.3
+0.3
-0.5
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
+0.7
+0.1
+0.4
+0.3
+0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.9
+0.5
+0.1
+0.2
+0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+1.0
-0.0
+0.5
+0.5
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.3
+1.0
+0.4
-0.3
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+1.0
+1.5
+0.0
+0.6
-0.0
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