Fitness for 5 genes in Escherichia coli BW25113

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500 ntb2462 and maeB are separated by 292 nucleotidesmaeB and talA are separated by 288 nucleotidestalA and tktB are separated by 19 nucleotidestktB and ypfG are separated by 94 nucleotides b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827 b2462 b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399 maeB b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638 talA b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661 tktB b2466: ypfG - hypothetical protein (NCBI), at 2,579,756 to 2,580,799 ypfG
Group Condition b2462 b2463 b2464 b2465 b2466
b2462 maeB talA tktB ypfG
carbon source carbon source D-Glucose 0.25mM -0.3 -0.2 -0.9 +0.1 -0.8
phage T7_phage 0.00015625 MOI TC4 +0.1 -0.5 -0.4 -0.6 -0.7
carbon source Acetate (C) -0.1 -2.3 +0.7 -0.0 +0.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.1 -0.2 -0.3 -0.3 -0.9
carbon source acetate (C) -0.2 -2.6 +1.1 -0.0 +0.2
M9 Acetate 0.5% aerobic +0.0 -2.5 +1.2 -0.1 -0.0
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.5 -1.1 +0.5 -0.1 -0.2
M9 Acetate 0.5% aerobic +0.1 -2.6 +1.3 +0.0 +0.1
carbon source acetate (C) -0.4 -2.9 +1.6 +0.2 +0.3
carbon source Acetate (C) -0.1 -1.6 +0.6 -0.0 +0.1
carbon source a-Ketoglutaric (C) -0.3 -1.7 +0.8 +0.2 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 -1.1 +0.6 -0.1 -0.5
carbon source a-Ketoglutaric (C) -0.1 -1.6 +0.8 +0.2 +0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.7 +0.5 +0.1 -0.2
phage T7_phage 0.00015625 MOI TC3 +0.5 -0.6 -0.2 +0.2 -0.4
carbon source Glycolic Acid (C) +0.1 -2.5 +2.0 +0.2 -0.1
stress Cisplatin 0.1 mg/ml +0.2 -1.0 -0.2 +0.2 +0.5
carbon source L-Malic (C) -0.3 -1.2 +1.1 +0.1 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.4 -0.6 +1.4 +0.2 -0.6
carbon source L-Malic (C) -0.4 -0.7 +1.0 +0.0 +0.1
carbon source Glycolic Acid (C) +0.1 -2.5 +1.9 +0.3 +0.2
carbon source succinate (C) -0.2 -1.6 +1.9 +0.2 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 -0.8 +1.0 +0.3 -0.2
carbon source succinate (C) -0.1 -1.1 +1.6 +0.1 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.9 +1.1 +0.3 +0.4
stress 3-methyl-1-butanol 1.25 vol% +0.2 -0.4 +0.5 +0.4 +0.4
carbon source Guanosine 0.35306 mM (C) +0.2 +0.1 +0.6 +0.8 -0.3
carbon source Guanosine 0.35306 mM (C) +0.2 +0.0 +0.4 +1.0 -0.1
drt_type_3 DRT_type_3 -0.1 +0.4 +0.4 +0.5 +0.4
carbon source Guanosine 0.35306 mM (C) -0.1 +0.1 +1.1 +1.7 -0.1
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