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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
b2462 and maeB are separated by 292 nucleotides
maeB and talA are separated by 288 nucleotides
talA and tktB are separated by 19 nucleotides
tktB and ypfG are separated by 94 nucleotides
b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827
b2462
b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399
maeB
b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638
talA
b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661
tktB
b2466: ypfG - hypothetical protein (NCBI), at 2,579,756 to 2,580,799
ypfG
Group
Condition
b2462
b2463
b2464
b2465
b2466
b2462
maeB
talA
tktB
ypfG
carbon source
carbon source D-Glucose 0.25mM
-0.3
-0.2
-0.9
+0.1
-0.8
phage
T7_phage 0.00015625 MOI TC4
+0.1
-0.5
-0.4
-0.6
-0.7
carbon source
Acetate (C)
-0.1
-2.3
+0.7
-0.0
+0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.1
-0.2
-0.3
-0.3
-0.9
carbon source
acetate (C)
-0.2
-2.6
+1.1
-0.0
+0.2
M9 Acetate 0.5% aerobic
+0.0
-2.5
+1.2
-0.1
-0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.5
-1.1
+0.5
-0.1
-0.2
M9 Acetate 0.5% aerobic
+0.1
-2.6
+1.3
+0.0
+0.1
carbon source
acetate (C)
-0.4
-2.9
+1.6
+0.2
+0.3
carbon source
Acetate (C)
-0.1
-1.6
+0.6
-0.0
+0.1
carbon source
a-Ketoglutaric (C)
-0.3
-1.7
+0.8
+0.2
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.2
-1.1
+0.6
-0.1
-0.5
carbon source
a-Ketoglutaric (C)
-0.1
-1.6
+0.8
+0.2
+0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.7
+0.5
+0.1
-0.2
phage
T7_phage 0.00015625 MOI TC3
+0.5
-0.6
-0.2
+0.2
-0.4
carbon source
Glycolic Acid (C)
+0.1
-2.5
+2.0
+0.2
-0.1
stress
Cisplatin 0.1 mg/ml
+0.2
-1.0
-0.2
+0.2
+0.5
carbon source
L-Malic (C)
-0.3
-1.2
+1.1
+0.1
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.4
-0.6
+1.4
+0.2
-0.6
carbon source
L-Malic (C)
-0.4
-0.7
+1.0
+0.0
+0.1
carbon source
Glycolic Acid (C)
+0.1
-2.5
+1.9
+0.3
+0.2
carbon source
succinate (C)
-0.2
-1.6
+1.9
+0.2
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.5
-0.8
+1.0
+0.3
-0.2
carbon source
succinate (C)
-0.1
-1.1
+1.6
+0.1
+0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.9
+1.1
+0.3
+0.4
stress
3-methyl-1-butanol 1.25 vol%
+0.2
-0.4
+0.5
+0.4
+0.4
carbon source
Guanosine 0.35306 mM (C)
+0.2
+0.1
+0.6
+0.8
-0.3
carbon source
Guanosine 0.35306 mM (C)
+0.2
+0.0
+0.4
+1.0
-0.1
drt_type_3
DRT_type_3
-0.1
+0.4
+0.4
+0.5
+0.4
carbon source
Guanosine 0.35306 mM (C)
-0.1
+0.1
+1.1
+1.7
-0.1
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