Fitness for 5 genes in Escherichia coli BW25113

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500 nteutP and b2462 are separated by 12 nucleotidesb2462 and maeB are separated by 292 nucleotidesmaeB and talA are separated by 288 nucleotidestalA and tktB are separated by 19 nucleotides b2461: eutP - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 2,573,000 to 2,573,479 eutP b2462: b2462 - orf, hypothetical protein (VIMSS), at 2,573,492 to 2,573,827 b2462 b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399 maeB b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638 talA b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661 tktB
Group Condition b2461 b2462 b2463 b2464 b2465
eutP b2462 maeB talA tktB
carbon source acetate (C) -0.1 -0.2 -2.6 +1.1 -0.0
carbon source Acetate (C) +0.0 -0.1 -2.3 +0.7 -0.0
phage T7_phage 0.00015625 MOI TC4 -0.2 +0.1 -0.5 -0.4 -0.6
carbon source acetate (C) -0.0 -0.4 -2.9 +1.6 +0.2
M9 Acetate 0.5% aerobic -0.1 +0.0 -2.5 +1.2 -0.1
M9 Acetate 0.5% aerobic -0.2 +0.1 -2.6 +1.3 +0.0
carbon source Acetate (C) -0.1 -0.1 -1.6 +0.6 -0.0
carbon source a-Ketoglutaric (C) -0.1 -0.3 -1.7 +0.8 +0.2
phage T7_phage 0.00015625 MOI TC3 -0.8 +0.5 -0.6 -0.2 +0.2
stress Cisplatin 0.1 mg/ml -0.2 +0.2 -1.0 -0.2 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.4 -0.5 -1.1 +0.5 -0.1
carbon source a-Ketoglutaric (C) +0.1 -0.1 -1.6 +0.8 +0.2
carbon source L-Malic (C) -0.1 -0.3 -1.2 +1.1 +0.1
carbon source Glycolic Acid (C) -0.0 +0.1 -2.5 +2.0 +0.2
carbon source L-Malic (C) -0.1 -0.4 -0.7 +1.0 +0.0
carbon source Glycolic Acid (C) +0.1 +0.1 -2.5 +1.9 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.2 -1.1 +0.6 -0.1
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 -0.7 +0.0 -0.3 +0.6
carbon source succinate (C) -0.0 -0.2 -1.6 +1.9 +0.2
carbon source succinate (C) -0.1 -0.1 -1.1 +1.6 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 +0.3 -0.9 +1.1 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 -0.4 -0.6 +1.4 +0.2
stress LB Aerobic with Nitrofurantoin 0.00003125 mM +0.3 +0.3 -0.3 +0.8 -0.2
phage Ste41 +1.1 +0.3 +0.0 -0.7 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.2 +0.5 -0.8 +1.0 +0.3
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.6 -0.3 +0.4 +0.6 -0.0
stress 2,4-Dinitrophenol 1 mM +0.4 +0.8 +0.4 -0.2 -0.0
drt_type_3 DRT_type_3 +0.5 -0.1 +0.4 +0.4 +0.5
carbon source Guanosine 0.35306 mM (C) +0.2 +0.2 +0.0 +0.4 +1.0
carbon source Guanosine 0.35306 mM (C) +0.3 -0.1 +0.1 +1.1 +1.7
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