Fitness for 5 genes in Escherichia coli BW25113

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500 nteutG and eutJ overlap by 11 nucleotideseutJ and eutE are separated by 10 nucleotideseutE and cchB are separated by 11 nucleotidescchB and cchA are separated by 106 nucleotides b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533 eutG b2454: eutJ - predicted chaperonin, ethanolamine utilization protein (NCBI), at 2,567,523 to 2,568,359 eutJ b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773 eutE b2456: cchB - predicted carboxysome structural protein, ethanolamine utilization protein (NCBI), at 2,569,785 to 2,570,072 cchB b2457: cchA - detox protein (VIMSS), at 2,570,179 to 2,570,472 cchA
Group Condition b2453 b2454 b2455 b2456 b2457
eutG eutJ eutE cchB cchA
phage Ste41 -0.7 -0.9 -0.8 +0.3 -0.9
carbon source D-Glucosamine Hydrochloride (C) -0.2 +0.1 -0.9 -2.1 +0.1
motility outer cut, LB soft agar motility assay +0.2 -0.9 -0.0 -1.8 -0.1
carbon source acetate (C) -0.2 +0.5 -0.6 -2.0 -0.3
stress syringaldehyde 5 mM +0.0 -0.6 -0.3 -0.4 -1.0
carbon source carbon source D-Mannose 0.5mM +0.3 +0.3 -0.1 -2.6 -0.2
carbon source L-Malic (C) -0.1 +0.1 -0.2 -1.7 -0.2
phage Ste41 -0.6 -0.6 -0.5 -0.6 +0.2
protonated/deuterated deuterated glucose in H2O -0.2 +0.1 -0.2 -1.5 -0.2
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.1 +0.3 -0.7 -0.7 -0.7
carbon source acetate (C) -0.3 +0.0 +0.0 -1.8 +0.2
carbon source carbon source D-Mannose 5mM -0.1 -0.5 -0.1 -2.2 +1.2
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.3 -0.3 -0.1 -1.0 -0.5
phage RB68 -0.4 -0.7 -0.3 -1.0 +0.8
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.2 -0.1 +0.4 -1.5 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.6 -0.7 -0.5 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.1 -0.0 -0.3 -1.4 +0.5
stress acetate 140 mM -0.1 -0.6 +0.5 -0.8 -0.1
phage TP4 +0.3 +0.4 -0.4 -0.4 -1.0
phage TP4 +0.4 +0.5 +0.1 -0.9 -0.5
stress LB Anaerobic with Furaltadone 0.000015625 mM +0.0 +0.1 +0.4 -1.2 +0.6
carbon source carbon source D-Glucose 5mM +0.2 -0.5 +0.9 -0.7 +0.8
phage T7_phage 0.00015625 MOI TC4 -0.1 -0.3 +0.7 +1.2 -0.7
motility outer cut, LB soft agar motility assay +0.4 -0.1 +0.9 -0.7 +0.5
phage T7_phage 0.0015625 MOI TC1 -0.1 -0.3 +0.8 +1.2 -0.3
carbon source carbon source D-Mannose 20mM +0.5 +0.2 +0.7 -0.2 +0.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.0 +0.8 +0.6 +0.9 +0.8
stress 2,4-Dinitrophenol 1 mM -0.1 +0.6 +0.5 +1.7 +1.3
stress 2,4-Dinitrophenol 1 mM +0.1 +0.4 +0.5 +1.9 +1.7
stress Thallium(I) acetate 0.0625 mg/ml +1.0 +0.8 +0.7 +2.1 +0.2
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