Fitness for 5 genes in Escherichia coli BW25113

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500 ntalaX and alaW are separated by 39 nucleotidesalaW and yfeC are separated by 235 nucleotidesyfeC and yfeD are separated by 1 nucleotidesyfeD and gltX are separated by 51 nucleotides b2396: alaX - tRNA-Ala (NCBI), at 2,516,063 to 2,516,138 alaX b2397: alaW - tRNA-Ala (NCBI), at 2,516,178 to 2,516,253 alaW b2398: yfeC - orf, hypothetical protein (VIMSS), at 2,516,489 to 2,516,833 yfeC b2399: yfeD - predicted DNA-binding transcriptional regulator (RefSeq), at 2,516,835 to 2,517,227 yfeD b2400: gltX - glutamyl-tRNA synthetase (NCBI), at 2,517,279 to 2,518,694 gltX
Group Condition b2396 b2397 b2398 b2399 b2400
alaX alaW yfeC yfeD gltX
carbon source acetate (C) N.D. N.D. -0.9 -0.9 N.D.
msc 3hr_MSC_rep2_lowinoculum N.D. N.D. -1.3 -0.5 N.D.
carbon source acetate (C) N.D. N.D. -1.1 -0.6 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. N.D. -0.9 -0.7 N.D.
phage R2_N4_T2_phage10 N.D. N.D. -0.8 -0.8 N.D.
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM N.D. N.D. -1.2 -0.5 N.D.
carbon source Glycolic Acid (C) N.D. N.D. -1.0 -0.6 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -1.0 -0.5 N.D.
stress LB Anaerobic with Irgasan 0.000000625 mM N.D. N.D. -1.4 -0.1 N.D.
stress LB Anaerobic with Irgasan 0.000000625 mM N.D. N.D. -1.1 -0.3 N.D.
phage Phage_N4_cage_4_mouse_3_Cecum_40 hr N.D. N.D. -0.6 -0.8 N.D.
control NoPredeControl N.D. N.D. -1.0 -0.4 N.D.
protonated/deuterated deuterated glucose in H2O N.D. N.D. -0.8 -0.6 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. N.D. -1.0 -0.4 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. N.D. -1.4 +0.0 N.D.
carbon source carbon source D-Glucose 0.25mM N.D. N.D. -0.6 -0.8 N.D.
protonated/deuterated protonated control N.D. N.D. -0.8 -0.6 N.D.
phage R2_N4_T2_phage8 N.D. N.D. -0.7 -0.6 N.D.
stress Cisplatin 0.1 mg/ml N.D. N.D. -1.5 +0.8 N.D.
stress LB Anaerobic with Chloramphenicol 0.00000390625 mM N.D. N.D. -1.0 +0.3 N.D.
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. N.D. -1.0 +0.5 N.D.
control PBS_cage_2_mice _Feces_22 hr N.D. N.D. -0.9 +0.4 N.D.
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM N.D. N.D. +0.8 -0.5 N.D.
stress Cephalothin 0.01 mg/ml N.D. N.D. +1.3 +0.1 N.D.
lb LB N.D. N.D. +1.1 +0.4 N.D.
phage T7_phage 0.0015625 MOI TC1 N.D. N.D. +0.9 +0.8 N.D.
lb LB N.D. N.D. +1.3 +0.5 N.D.
phage phage N.D. N.D. +0.8 +1.0 N.D.
phage T7_phage 0.00015625 MOI TC4 N.D. N.D. +0.9 +0.9 N.D.
lb LB N.D. N.D. +1.7 +0.2 N.D.
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