Fitness for 5 genes in Escherichia coli BW25113

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500 ntypdI and yfdY are separated by 76 nucleotidesyfdY and ddg are separated by 352 nucleotidesddg and ypdK are separated by 355 nucleotidesypdK and yfdZ are separated by 64 nucleotides b2376: ypdI - predicted lipoprotein involved in colanic acid biosynthesis (NCBI), at 2,492,720 to 2,492,995 ypdI b2377: yfdY - predicted inner membrane protein (NCBI), at 2,493,072 to 2,493,314 yfdY b2378: ddg - putative heat shock protein (VIMSS), at 2,493,667 to 2,494,587 ddg b4680: ypdK - expressed protein (RefSeq), at 2,494,943 to 2,495,014 ypdK b2379: yfdZ - hypothetical protein (NCBI), at 2,495,079 to 2,496,317 yfdZ
Group Condition b2376 b2377 b2378 b4680 b2379
ypdI yfdY ddg ypdK yfdZ
phage T7_phage 0.0015625 MOI TC2 +1.0 -5.3 -0.4 N.D. -0.4
stress LB Anaerobic with Novobiocin 0.00003125 mM -0.9 -0.4 -3.4 N.D. -0.3
stress LB Anaerobic with Novobiocin 0.000125 mM -0.3 -0.3 -3.4 N.D. -0.2
stress LB Anaerobic with Novobiocin 0.00003125 mM -0.6 -0.0 -3.5 N.D. -0.1
stress LB Anaerobic with Novobiocin 0.000125 mM -0.3 +0.3 -3.6 N.D. -0.4
phage Lambda_ci1857 -0.6 -1.8 -0.1 N.D. +0.0
carbon source Guanosine 0.35306 mM (C) -1.7 -0.9 +0.2 N.D. +0.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -1.5 -1.0 +0.1 N.D. +0.2
carbon source carbon source D-Mannose 0.5mM -3.0 +0.9 +0.1 N.D. -0.3
stress LB Aerobic with Novobiocin 0.0000625 mM -0.4 -0.8 -1.0 N.D. -0.1
phage T7_phage 0.00015625 MOI TC3 +1.9 -2.4 -0.7 N.D. -0.6
stress LB Aerobic with Trimethoprim 0.00000125 mM -1.0 -1.1 +0.1 N.D. +0.2
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.3 -1.1 -0.5 N.D. -0.3
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -1.7 -0.3 +0.3 N.D. +0.1
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -1.4 -0.5 +0.2 N.D. +0.2
stress LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM -1.2 -0.5 +0.3 N.D. +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.9 +0.1 +0.0 N.D. +0.5
carbon source carbon source D-Mannose 1mM -1.3 +0.7 -0.0 N.D. -0.2
predation 3 hr rep1_Tiberius_100fold_dilution -1.5 +0.6 +0.3 N.D. +0.3
stress LB Anaerobic with Spectinomycin 0.00003125 mM -1.4 +0.5 +0.4 N.D. +0.2
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.6 +1.3 -0.6 N.D. -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 +1.1 +0.3 N.D. +0.1
carbon source carbon source D-Glucose 0.25mM -0.9 +1.2 +0.3 N.D. -0.0
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -1.2 +1.4 -0.1 N.D. +0.4
nophagecontrol R1_N4_T1_phage0 -0.7 +1.0 -0.1 N.D. +0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.8 +0.1 +1.2 N.D. +0.2
carbon source carbon source D-Glucose 0.25mM -0.5 +1.6 +0.1 N.D. -0.3
predation 6 hr rep1_HD100_10fold_dilution_grewON +1.2 -0.2 +0.6 N.D. -0.2
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.7 +1.1 +0.5 N.D. +0.6
stress Aluminum chloride 5 mM +0.1 +1.7 +1.7 N.D. +0.1
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