Fitness for 5 genes in Escherichia coli BW25113

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500 ntyfdX and ypdI are separated by 295 nucleotidesypdI and yfdY are separated by 76 nucleotidesyfdY and ddg are separated by 352 nucleotidesddg and ypdK are separated by 355 nucleotides b2375: yfdX - hypothetical protein (NCBI), at 2,491,789 to 2,492,424 yfdX b2376: ypdI - predicted lipoprotein involved in colanic acid biosynthesis (NCBI), at 2,492,720 to 2,492,995 ypdI b2377: yfdY - predicted inner membrane protein (NCBI), at 2,493,072 to 2,493,314 yfdY b2378: ddg - putative heat shock protein (VIMSS), at 2,493,667 to 2,494,587 ddg b4680: ypdK - expressed protein (RefSeq), at 2,494,943 to 2,495,014 ypdK
Group Condition b2375 b2376 b2377 b2378 b4680
yfdX ypdI yfdY ddg ypdK
stress LB Anaerobic with Novobiocin 0.00003125 mM -0.4 -0.9 -0.4 -3.4 N.D.
stress LB Anaerobic with Novobiocin 0.00003125 mM -0.4 -0.6 -0.0 -3.5 N.D.
stress LB Anaerobic with Novobiocin 0.000125 mM -0.3 -0.3 -0.3 -3.4 N.D.
phage T7_phage 0.0015625 MOI TC2 +0.5 +1.0 -5.3 -0.4 N.D.
stress LB Anaerobic with Novobiocin 0.000125 mM -0.5 -0.3 +0.3 -3.6 N.D.
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.4 -1.5 -1.0 +0.1 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.7 -1.4 +0.1 -0.4 N.D.
M9 Acetate 0.5% aerobic -1.0 -1.2 +0.2 -0.2 N.D.
lb LB, Aerobic -0.5 -1.4 -0.6 +0.2 N.D.
stress LB Aerobic with Trimethoprim 0.00000125 mM -0.3 -1.0 -1.1 +0.1 N.D.
stress LB Anaerobic with Oxytetracycline 0.00000015625 mM -0.5 -1.4 -0.5 +0.2 N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -0.5 -1.7 -0.3 +0.3 N.D.
carbon source carbon source D-Mannose 0.5mM -0.1 -3.0 +0.9 +0.1 N.D.
carbon source Guanosine 0.35306 mM (C) +0.3 -1.7 -0.9 +0.2 N.D.
carbon source carbon source D-Mannose 1mM -1.4 -1.3 +0.7 -0.0 N.D.
stress LB Anaerobic with Ramoplanin 0.0000625 mM -0.6 -1.6 +0.4 -0.1 N.D.
phage Lambda_ci1857 +0.7 -0.6 -1.8 -0.1 N.D.
phage T7_phage 0.00015625 MOI TC3 -0.1 +1.9 -2.4 -0.7 N.D.
predation 3 hr rep1_Tiberius_100fold_dilution -0.6 -1.5 +0.6 +0.3 N.D.
phage Ste41 +1.0 -0.4 -1.3 +0.1 N.D.
nophagecontrol R1_N4_T1_phage0 -0.6 -0.7 +1.0 -0.1 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.6 -0.8 +0.1 +1.2 N.D.
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.1 -1.2 +1.4 -0.1 N.D.
carbon source carbon source D-Glucose 0.25mM -0.5 -0.9 +1.2 +0.3 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -1.2 +1.1 +0.3 N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.8 -0.6 +1.3 -0.6 N.D.
carbon source carbon source D-Glucose 0.25mM +0.6 -0.5 +1.6 +0.1 N.D.
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.7 +1.2 -0.2 +0.6 N.D.
phage Papa dilution 10-1 cacl2 maltose +1.6 +0.6 +0.4 -0.0 N.D.
stress Aluminum chloride 5 mM -0.3 +0.1 +1.7 +1.7 N.D.
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