Fitness for 5 genes in Escherichia coli BW25113

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500 ntdsdC and dsdX are separated by 217 nucleotidesdsdX and dsdA are separated by 17 nucleotidesdsdA and emrY are separated by 107 nucleotidesemrY and emrK overlap by 1 nucleotides b2364: dsdC - DNA-binding transcriptional dual regulator (NCBI), at 2,474,716 to 2,475,651 dsdC b2365: dsdX - predicted transporter (NCBI), at 2,475,869 to 2,477,206 dsdX b2366: dsdA - D-serine dehydratase (NCBI), at 2,477,224 to 2,478,552 dsdA b2367: emrY - predicted multidrug efflux system (NCBI), at 2,478,660 to 2,480,198 emrY b2368: emrK - EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI), at 2,480,198 to 2,481,361 emrK
Group Condition b2364 b2365 b2366 b2367 b2368
dsdC dsdX dsdA emrY emrK
nitrogen source D-Serine (N) -3.8 +1.0 -4.7 -0.2 +0.1
carbon source D-Serine (C) -3.3 +0.6 -4.5 -0.0 +0.2
carbon source D-Serine (C) -2.9 +0.8 -4.3 +0.1 +0.2
nitrogen source D-Serine (N) -3.6 +1.0 -3.4 -0.2 +0.1
carbon source carbon source D-Glucose 0.25mM -0.9 -0.4 -1.7 -0.3 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 -0.3 -1.3 -0.6 -0.7
carbon source carbon source D-Mannose 1mM -0.3 -0.3 -1.4 -0.4 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.1 -0.5 -0.9 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.5 -1.0 -0.5 +0.1
stress Cisplatin 0.1 mg/ml +0.1 -0.3 -1.4 -0.4 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.0 -0.4 -0.9 -0.3 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 -0.4 -0.7 -0.2 -0.5
gfp_control GFP_control -0.5 +0.3 -0.4 -0.8 -0.6
phage Δdbn_10 +0.5 -0.3 -1.2 -0.3 -0.6
carbon source carbon source D-Glucose 5mM -0.4 -0.3 -0.7 -0.8 +0.4
motility outer cut, LB soft agar motility assay +0.2 -0.1 -1.2 -0.5 -0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.3 +0.3 -1.2 -0.3 -0.1
stress Cisplatin 0.1 mg/ml +0.6 +0.4 -1.5 -0.6 -0.2
stress Cephalothin 0.01 mg/ml -0.1 +0.1 -2.2 +0.5 +0.3
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.3 -0.6 +0.3 -0.5 -0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.6 +0.3 +0.4 +0.0 -0.6
phage Ste41 +0.6 +0.1 -1.3 +0.4 -0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.5 +0.2 -1.5 +0.4 +0.2
phage N4_phage 0.09375 MOI TC1 -0.6 +0.2 -0.4 +0.7 +0.0
control PBS_cage_1_mouse_2_Cecum_40 hr +0.9 -0.3 -0.7 +0.4 +0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.2 +0.2 -0.7 +0.5 +0.5
stress Trimethoprim 0.005 mM -0.1 -0.3 -0.2 +1.1 +0.5
phage T7_phage 0.00015625 MOI TC4 +0.6 +0.3 -0.8 +0.4 +0.8
control PBS_cage_1_mice _Feces_16 hr +1.2 -0.3 -0.0 +0.7 +0.2
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.2 +1.2 +0.5 +0.3
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