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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
dsdC and dsdX are separated by 217 nucleotides
dsdX and dsdA are separated by 17 nucleotides
dsdA and emrY are separated by 107 nucleotides
emrY and emrK overlap by 1 nucleotides
b2364: dsdC - DNA-binding transcriptional dual regulator (NCBI), at 2,474,716 to 2,475,651
dsdC
b2365: dsdX - predicted transporter (NCBI), at 2,475,869 to 2,477,206
dsdX
b2366: dsdA - D-serine dehydratase (NCBI), at 2,477,224 to 2,478,552
dsdA
b2367: emrY - predicted multidrug efflux system (NCBI), at 2,478,660 to 2,480,198
emrY
b2368: emrK - EmrKY-TolC multidrug resistance efflux pump, membrane fusion protein component (NCBI), at 2,480,198 to 2,481,361
emrK
Group
Condition
b2364
b2365
b2366
b2367
b2368
dsdC
dsdX
dsdA
emrY
emrK
nitrogen source
D-Serine (N)
-3.8
+1.0
-4.7
-0.2
+0.1
carbon source
D-Serine (C)
-3.3
+0.6
-4.5
-0.0
+0.2
carbon source
D-Serine (C)
-2.9
+0.8
-4.3
+0.1
+0.2
nitrogen source
D-Serine (N)
-3.6
+1.0
-3.4
-0.2
+0.1
carbon source
carbon source D-Glucose 0.25mM
-0.9
-0.4
-1.7
-0.3
-0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.3
-0.3
-1.3
-0.6
-0.7
carbon source
carbon source D-Mannose 1mM
-0.3
-0.3
-1.4
-0.4
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.1
-0.5
-0.9
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.3
-0.5
-1.0
-0.5
+0.1
stress
Cisplatin 0.1 mg/ml
+0.1
-0.3
-1.4
-0.4
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.0
-0.4
-0.9
-0.3
-0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
-0.4
-0.7
-0.2
-0.5
gfp_control
GFP_control
-0.5
+0.3
-0.4
-0.8
-0.6
phage
Δdbn_10
+0.5
-0.3
-1.2
-0.3
-0.6
carbon source
carbon source D-Glucose 5mM
-0.4
-0.3
-0.7
-0.8
+0.4
motility
outer cut, LB soft agar motility assay
+0.2
-0.1
-1.2
-0.5
-0.1
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.3
+0.3
-1.2
-0.3
-0.1
stress
Cisplatin 0.1 mg/ml
+0.6
+0.4
-1.5
-0.6
-0.2
stress
Cephalothin 0.01 mg/ml
-0.1
+0.1
-2.2
+0.5
+0.3
stress
Ciprofloxacin Hydrochloride 0.0003 mM
+0.3
-0.6
+0.3
-0.5
-0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.6
+0.3
+0.4
+0.0
-0.6
phage
Ste41
+0.6
+0.1
-1.3
+0.4
-0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.5
+0.2
-1.5
+0.4
+0.2
phage
N4_phage 0.09375 MOI TC1
-0.6
+0.2
-0.4
+0.7
+0.0
control
PBS_cage_1_mouse_2_Cecum_40 hr
+0.9
-0.3
-0.7
+0.4
+0.0
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.2
+0.2
-0.7
+0.5
+0.5
stress
Trimethoprim 0.005 mM
-0.1
-0.3
-0.2
+1.1
+0.5
phage
T7_phage 0.00015625 MOI TC4
+0.6
+0.3
-0.8
+0.4
+0.8
control
PBS_cage_1_mice _Feces_16 hr
+1.2
-0.3
-0.0
+0.7
+0.2
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.2
+1.2
+0.5
+0.3
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