Fitness for 5 genes in Escherichia coli BW25113

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500 ntoweS and yfdP are separated by 18 nucleotidesyfdP and yfdQ are separated by 65 nucleotidesyfdQ and b2361 are separated by 127 nucleotidesb2361 and yfdS overlap by 10 nucleotides b2358: oweS - CPS-53 (KpLE1) prophage; predicted defective phage replication protein O (RefSeq), at 2,470,900 to 2,471,607 oweS b2359: yfdP - CPS-53 (KpLE1) prophage; predicted protein (RefSeq), at 2,471,626 to 2,471,988 yfdP b2360: yfdQ - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,472,054 to 2,472,878 yfdQ b2361: b2361 - orf, hypothetical protein (VIMSS), at 2,473,006 to 2,473,542 b2361 b2362: yfdS - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,533 to 2,473,895 yfdS
Group Condition b2358 b2359 b2360 b2361 b2362
oweS yfdP yfdQ b2361 yfdS
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.0 -1.2 -0.4 +0.2 -1.0
control PBS_cage_1_mouse_1_Cecum_40 hr -1.5 -0.2 -0.2 -0.1 -1.1
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.4 -0.2 -0.2 -2.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -1.0 -0.3 -0.0 -1.7
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.1 -1.0 -0.4 +0.1 -1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.1 -0.6 -0.2 +0.0 -1.4
motility outer cut, LB soft agar motility assay -0.8 -0.4 -0.4 -0.4 -0.1
carbon source Ying_sugar7 rep C; time point 3 -0.4 -0.7 -0.2 -0.0 -0.7
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.1 -0.9 -0.2 +0.1 -0.9
carbon source carbon source D-Glucose 0.25mM +0.0 -1.2 +0.2 +0.2 -0.9
motility outer cut, LB soft agar motility assay -0.7 +0.2 -0.3 -0.1 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 -0.3 -0.0 -0.2 -1.3
mzaabcde mzaABCDE -0.5 +0.2 +0.0 +0.1 -1.2
stress Benzalkonium Chloride 0.004 mg/ml -0.6 -0.9 -0.1 +0.3 +0.1
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.0 -1.2 -0.2 -0.1 +0.5
stress toluene 2.5 vol% +0.4 -0.6 +0.2 -0.2 -0.9
carbon source carbon source D-Mannose 0.5mM -0.8 -0.5 -0.1 -0.3 +0.6
carbon source carbon source D-Glucose 0.25mM -0.4 +0.4 +0.3 +0.2 -1.4
carbon source carbon source D-Glucose 5mM -0.6 +0.5 -0.5 +0.2 +0.2
stress octanol 0.08 vol% +0.9 -1.0 +0.3 -0.2 +0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.9 +0.4 -0.1 -0.2 -0.6
carbon source carbon source D-Mannose 5mM -0.2 +0.3 -0.6 -0.0 +1.1
carbon source carbon source D-Mannose 5mM -0.5 -0.2 +0.4 +0.3 +0.7
phage Ste41 +0.4 -0.9 +0.4 +0.2 +0.4
phage T7_phage 0.0015625 MOI TC2 -0.4 +0.7 +0.7 +0.2 -0.3
phage Ste41 -0.4 +0.5 +0.9 +0.5 -0.1
phage T7_phage 0.0015625 MOI TC1 +0.9 +0.6 +0.3 +0.2 -0.1
phage T7_phage 0.00015625 MOI TC3 +0.1 +0.2 +0.2 +0.6 +1.0
carbon source carbon source D-Glucose 1mM +0.2 +0.3 -0.0 +0.3 +1.3
phage T7_phage 0.00015625 MOI TC4 +0.7 +1.2 +0.1 +0.5 +0.2
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