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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
oweS and yfdP are separated by 18 nucleotides
yfdP and yfdQ are separated by 65 nucleotides
yfdQ and b2361 are separated by 127 nucleotides
b2361 and yfdS overlap by 10 nucleotides
b2358: oweS - CPS-53 (KpLE1) prophage; predicted defective phage replication protein O (RefSeq), at 2,470,900 to 2,471,607
oweS
b2359: yfdP - CPS-53 (KpLE1) prophage; predicted protein (RefSeq), at 2,471,626 to 2,471,988
yfdP
b2360: yfdQ - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,472,054 to 2,472,878
yfdQ
b2361: b2361 - orf, hypothetical protein (VIMSS), at 2,473,006 to 2,473,542
b2361
b2362: yfdS - CPS-53 (KpLE1) prophage; predicted protein (NCBI), at 2,473,533 to 2,473,895
yfdS
Group
Condition
b2358
b2359
b2360
b2361
b2362
oweS
yfdP
yfdQ
b2361
yfdS
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.0
-1.2
-0.4
+0.2
-1.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-1.5
-0.2
-0.2
-0.1
-1.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.4
-0.2
-0.2
-2.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-1.0
-0.3
-0.0
-1.7
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.1
-1.0
-0.4
+0.1
-1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.1
-0.6
-0.2
+0.0
-1.4
motility
outer cut, LB soft agar motility assay
-0.8
-0.4
-0.4
-0.4
-0.1
carbon source
Ying_sugar7 rep C; time point 3
-0.4
-0.7
-0.2
-0.0
-0.7
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.1
-0.9
-0.2
+0.1
-0.9
carbon source
carbon source D-Glucose 0.25mM
+0.0
-1.2
+0.2
+0.2
-0.9
motility
outer cut, LB soft agar motility assay
-0.7
+0.2
-0.3
-0.1
-0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
-0.3
-0.0
-0.2
-1.3
mzaabcde
mzaABCDE
-0.5
+0.2
+0.0
+0.1
-1.2
stress
Benzalkonium Chloride 0.004 mg/ml
-0.6
-0.9
-0.1
+0.3
+0.1
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.0
-1.2
-0.2
-0.1
+0.5
stress
toluene 2.5 vol%
+0.4
-0.6
+0.2
-0.2
-0.9
carbon source
carbon source D-Mannose 0.5mM
-0.8
-0.5
-0.1
-0.3
+0.6
carbon source
carbon source D-Glucose 0.25mM
-0.4
+0.4
+0.3
+0.2
-1.4
carbon source
carbon source D-Glucose 5mM
-0.6
+0.5
-0.5
+0.2
+0.2
stress
octanol 0.08 vol%
+0.9
-1.0
+0.3
-0.2
+0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.9
+0.4
-0.1
-0.2
-0.6
carbon source
carbon source D-Mannose 5mM
-0.2
+0.3
-0.6
-0.0
+1.1
carbon source
carbon source D-Mannose 5mM
-0.5
-0.2
+0.4
+0.3
+0.7
phage
Ste41
+0.4
-0.9
+0.4
+0.2
+0.4
phage
T7_phage 0.0015625 MOI TC2
-0.4
+0.7
+0.7
+0.2
-0.3
phage
Ste41
-0.4
+0.5
+0.9
+0.5
-0.1
phage
T7_phage 0.0015625 MOI TC1
+0.9
+0.6
+0.3
+0.2
-0.1
phage
T7_phage 0.00015625 MOI TC3
+0.1
+0.2
+0.2
+0.6
+1.0
carbon source
carbon source D-Glucose 1mM
+0.2
+0.3
-0.0
+0.3
+1.3
phage
T7_phage 0.00015625 MOI TC4
+0.7
+1.2
+0.1
+0.5
+0.2
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