Fitness for 5 genes in Escherichia coli BW25113

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500 ntargT and ubiX are separated by 265 nucleotidesubiX and purF are separated by 94 nucleotidespurF and cvpA are separated by 36 nucleotidescvpA and dedD are separated by 258 nucleotides b2310: argT - lysine/arginine/ornithine transporter subunit (NCBI), at 2,425,031 to 2,425,813 argT b2311: ubiX - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase (NCBI), at 2,426,079 to 2,426,648 ubiX b2312: purF - amidophosphoribosyltransferase (NCBI), at 2,426,743 to 2,428,260 purF b2313: cvpA - membrane protein required for colicin V production (NCBI), at 2,428,297 to 2,428,785 cvpA b2314: dedD - putative lipoprotein (VIMSS), at 2,429,044 to 2,429,706 dedD
Group Condition b2310 b2311 b2312 b2313 b2314
argT ubiX purF cvpA dedD
carbon source carbon source D-Glucose 0.25mM -0.0 N.D. -7.4 -3.5 -1.0
carbon source Ying_sugar7 rep C; time point 3 +0.4 N.D. -8.2 -4.2 +0.1
carbon source Ying_sugar7 rep B; time point 3 +0.3 N.D. -8.3 -3.3 -0.2
msc 6hr_MSC_rep1_lowinoculum +0.2 N.D. -7.9 -3.0 -0.4
carbon source Ying_AminoAcid20 rep C; time point 3 -0.1 N.D. -8.3 -2.6 +0.1
msc 22hr_MSC_rep1_lowinoculum +0.3 N.D. -8.4 -2.6 -0.2
msc 22hr_MSC_rep2_lowinoculum -0.0 N.D. -8.5 -2.1 -0.1
carbon source Ying_sugar7 rep A; time point 2 +0.2 N.D. -8.4 -2.4 +0.0
carbon source Ying_sugar7 rep A; time point 3 +0.3 N.D. -8.3 -2.4 -0.1
carbon source Ying_sugar7 rep C; time point 2 -0.0 N.D. -8.4 -2.4 +0.4
carbon source Ying_sugar7 rep B; time point 2 +0.1 N.D. -8.4 -2.5 +0.4
carbon source Ying_AminoAcid20 rep A; time point 3 -0.1 N.D. -8.4 -1.9 +0.1
carbon source Ying_AminoAcid20 rep B; time point 3 -0.1 N.D. -7.8 -2.2 -0.1
carbon source Ying_AminoAcid20 rep B; time point 2 -0.0 N.D. -8.4 -1.0 -0.0
carbon source carbon source D-Glucose 1mM -0.1 N.D. -7.7 -0.8 -0.7
carbon source Ying_AminoAcid20 rep A; time point 2 -0.2 N.D. -7.3 -1.9 -0.0
carbon source Ying_OrganicAcid7 rep A; time point 3 +0.4 N.D. -8.2 -1.5 +0.4
msc 6hr_MSC_rep2_lowinoculum +0.1 N.D. -7.2 -1.5 -0.3
carbon source Ying_OrganicAcid7 rep C; time point 3 +0.2 N.D. -8.2 -1.0 +0.2
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.1 N.D. -7.9 -1.2 +0.1
carbon source Ying_AminoAcid20 rep C; time point 2 +0.2 N.D. -6.5 -2.4 -0.1
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 0.5x -0.0 N.D. -6.6 -2.1 +0.2
nitrogen source Gly-DL-Asp (N) -0.3 N.D. -5.5 -3.2 +0.6
nitrogen source Gly-DL-Asp (N) -0.0 N.D. -6.2 -2.1 +0.6
carbon source Ying_OrganicAcid7 rep A; time point 2 +0.2 N.D. -6.9 -1.3 +0.5
nitrogen source L-Aspartic Acid (N) -0.3 N.D. -6.2 -1.7 +1.1
carbon source D-Glucose-6-Phosphate (C) -0.2 N.D. -5.7 -2.2 +1.2
carbon source carbon source D-Mannose 1mM -0.2 N.D. -7.5 +0.4 +0.8
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.0 N.D. -0.9 -5.0 +4.6
stress Bacitracin 1 mg/ml +0.1 N.D. -0.7 -4.0 +4.2
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