Fitness for 5 genes in Escherichia coli BW25113

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500 ntyfcF and yfcG are separated by 135 nucleotidesyfcG and folX are separated by 56 nucleotidesfolX and yfcH are separated by 20 nucleotidesyfcH and yfcI are separated by 47 nucleotides b2301: yfcF - predicted enzyme (NCBI), at 2,417,863 to 2,418,507 yfcF b2302: yfcG - predicted glutathione S-transferase (NCBI), at 2,418,643 to 2,419,290 yfcG b2303: folX - D-erythro-7,8-dihydroneopterin triphosphate 2'-epimerase and dihydroneopterin aldolase (NCBI), at 2,419,347 to 2,419,709 folX b2304: yfcH - conserved protein with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,419,730 to 2,420,623 yfcH b2305: yfcI - hypothetical protein (NCBI), at 2,420,671 to 2,421,561 yfcI
Group Condition b2301 b2302 b2303 b2304 b2305
yfcF yfcG folX yfcH yfcI
motility outer cut, LB soft agar motility assay -1.3 -0.2 -0.6 +0.1 -0.3
stress Cisplatin 0.1 mg/ml -0.9 -0.3 -0.7 +0.1 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 +0.1 -0.3 -0.3 +0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 +0.2 -0.3 -0.3 -0.6
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 -0.2 -0.7 -0.3 +0.1
stress 3-methyl-1-butanol 1.25 vol% -0.9 -0.5 +0.0 +0.2 +0.2
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.4 -0.3 -0.2 -0.3 -0.4
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.2 +0.2 +0.4 -0.4 +0.3
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.6 +0.4 +0.2 +0.1 -0.6
stress Benzalkonium Chloride 0.004 mg/ml +0.7 +0.4 -0.8 +0.1 -0.4
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.9 +0.1 -0.1 -0.1 -0.5
stress LB Aerobic with Trimethoprim 0.00000125 mM +0.5 +0.3 +0.4 -0.2 -0.4
phage T7_phage 0.00015625 MOI TC4 +0.7 -0.3 -0.0 -0.0 +0.5
phage Δdbn_10 +0.5 -0.3 +0.3 -0.2 +0.6
control PBS_cage_1_mouse_1_Cecum_40 hr +0.1 +0.2 +0.3 +0.8 -0.3
carbon source Ying_Nucleobase14 rep B; time point 3 -0.3 +0.1 +0.9 +0.1 +0.3
stress LB Anaerobic with Sulfamonomethoxine 0.0000625 mM +0.1 -0.2 +1.2 +0.1 -0.0
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.2 -0.0 -0.0 -0.2 +0.2
carbon source succinate (C) -0.2 +0.2 +1.0 +0.3 -0.1
carbon source Glycolic Acid (C) +0.1 +0.2 +0.9 +0.3 -0.0
stress Cephalothin 0.01 mg/ml +1.6 +0.0 -0.3 -0.1 +0.2
stress Carbenicillin 0.008 mg/ml +0.8 +0.4 +0.2 +0.1 -0.0
carbon source carbon source D-Mannose 5mM +0.6 +0.1 +0.3 +0.5 +0.1
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +1.1 +0.2 +0.1 +0.1 +0.2
carbon source Ying_Nucleobase14 rep C; time point 3 +0.4 +0.2 +1.0 +0.0 +0.2
phage T7_phage 0.0015625 MOI TC2 +1.4 -0.2 +0.1 +0.2 +0.3
stress M9 with Methotrexate 1.98 mM 48 hr +0.0 +0.0 +1.9 +0.0 +0.0
carbon source carbon source D-Glucose 5mM +0.5 +0.4 +0.7 +0.5 +0.1
stress M9 with Methotrexate 1.98 mM 24 hr -0.1 +0.1 +1.9 +0.1 +0.1
stress M9 with Methotrexate 1.98 mM 24 hr +0.1 +0.0 +2.8 +0.0 +0.0
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