Fitness for 5 genes in Escherichia coli BW25113

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500 ntnuoE and nuoC are separated by 2 nucleotidesnuoC and nuoB are separated by 105 nucleotidesnuoB and nuoA are separated by 15 nucleotidesnuoA and lrhA are separated by 630 nucleotides b2285: nuoE - NADH dehydrogenase subunit E (NCBI), at 2,399,574 to 2,400,074 nuoE b2286: nuoC - NADH:ubiquinone oxidoreductase, chain C,D (RefSeq), at 2,400,077 to 2,401,867 nuoC b2287: nuoB - NADH dehydrogenase subunit B (NCBI), at 2,401,973 to 2,402,635 nuoB b2288: nuoA - NADH dehydrogenase I chain A (VIMSS), at 2,402,651 to 2,403,094 nuoA b2289: lrhA - DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI), at 2,403,725 to 2,404,663 lrhA
Group Condition b2285 b2286 b2287 b2288 b2289
nuoE nuoC nuoB nuoA lrhA
stress 2,4-Dinitrophenol 2 mM -5.7 -6.2 -5.5 -5.0 +0.1
carbon source Ying_AminoAcid20 rep B; time point 3 -5.7 -5.5 -5.4 -3.6 -0.9
carbon source Ying_AminoAcid20 rep C; time point 3 -3.8 -5.6 -5.0 -2.6 -1.0
carbon source L-Malic (C) -3.3 -4.6 -3.5 -4.8 -1.2
carbon source acetate (C) -4.6 -4.7 -3.6 -2.9 -1.0
carbon source a-Ketoglutaric (C) -4.3 -4.3 -3.2 -2.9 -1.5
stress 2,4-Dinitrophenol 2 mM -2.4 -4.7 -5.0 -3.8 -0.3
carbon source Ying_AminoAcid20 rep A; time point 3 -2.0 -4.9 -4.4 -3.7 -1.1
carbon source Ying_Nucleobase14 rep A; time point 3 -5.8 -2.3 -4.4 -1.9 -1.6
carbon source D-Lactate (C) -3.6 -3.4 -4.1 -4.0 -0.7
carbon source L-Malic (C) -4.4 -3.3 -3.7 -2.9 -1.4
carbon source D-Lactate (C) -4.7 -3.5 -3.8 -2.3 -0.7
carbon source casaminos (C) -3.1 -4.5 -3.8 -3.0 -0.6
carbon source casaminos (C) -4.7 -3.9 -2.5 -3.2 -0.6
carbon source acetate (C) -3.3 -3.9 -2.9 -3.2 -1.0
carbon source Ying_AminoAcid20 rep B; time point 2 -3.3 -3.6 -3.6 -2.9 -0.9
carbon source succinate (C) -3.3 -4.0 -3.2 -2.5 -1.2
carbon source a-Ketoglutaric (C) -3.2 -4.1 -2.9 -2.5 -1.3
carbon source D-Ribose (C) -3.6 -3.7 -2.9 -2.8 -1.0
carbon source Ying_AminoAcid20 rep A; time point 2 -2.6 -3.5 -4.4 -2.4 -1.0
carbon source Ying_AminoAcid20 rep C; time point 2 -3.2 -3.5 -3.3 -3.0 -0.7
carbon source Dodecanol (C) -2.6 -3.2 -5.9 -1.8 -0.1
carbon source D-Ribose (C) -4.6 -2.8 -1.9 -3.3 -1.0
motility outer cut, LB soft agar motility assay -1.1 -0.8 -1.5 -3.9 +8.0
plasmid transformation mariner vector transformation; liquid outgrowth assay +5.0 +4.0 +3.4 +1.6 -0.2
plasmid transformation mariner vector transformation; agar assay +4.3 +4.1 +4.2 +3.3 +0.0
plasmid transformation vcDART vector transformation; agar assay +4.3 +4.2 +4.3 +3.2 +0.1
plasmid transformation mariner vector transformation; agar assay +4.0 +4.3 +4.4 +3.5 -0.2
phage T7_phage 0.00015625 MOI TC4 +4.2 +4.4 +4.5 +3.7 +1.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +6.8 +5.5 +4.8 +4.1 -0.1
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