Fitness for 5 genes in Escherichia coli BW25113

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500 ntelaD and yfbK are separated by 70 nucleotidesyfbK and yfbL are separated by 137 nucleotidesyfbL and yfbM are separated by 102 nucleotidesyfbM and yfbN are separated by 272 nucleotides b2269: elaD - predicted enzyme (NCBI), at 2,380,735 to 2,381,946 elaD b2270: yfbK - hypothetical protein (NCBI), at 2,382,017 to 2,383,744 yfbK b2271: yfbL - putative aminopeptidase (VIMSS), at 2,383,882 to 2,384,853 yfbL b2272: yfbM - hypothetical protein (NCBI), at 2,384,956 to 2,385,459 yfbM b2273: yfbN - hypothetical protein (NCBI), at 2,385,732 to 2,386,448 yfbN
Group Condition b2269 b2270 b2271 b2272 b2273
elaD yfbK yfbL yfbM yfbN
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.6 -1.1 -1.2 N.D.
carbon source carbon source D-Glucose 0.25mM N.D. -0.5 -0.8 -1.4 N.D.
stress Benzalkonium Chloride 0.004 mg/ml N.D. -0.1 -0.5 -2.0 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. -0.4 -0.6 -1.3 N.D.
M9 Acetate 0.5% aerobic N.D. -0.2 -1.4 -0.7 N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr N.D. -0.1 -0.6 -1.5 N.D.
M9 Acetate 0.5% aerobic N.D. -0.2 -1.4 -0.5 N.D.
predation 3 hr rep1_Tiberius_100fold_dilution N.D. -0.1 -0.8 -1.2 N.D.
stress LB Anaerobic with Spiramycin 0.00003125 mM N.D. -0.5 -0.7 -0.9 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.2 -0.6 -1.2 N.D.
nopredatorcontrol 3 hr NoPredatorControl N.D. -0.1 -0.6 -1.2 N.D.
M9 Glucose 2%, 20 mM NO3- anaerobic N.D. -0.1 -1.0 -0.8 N.D.
M9 Glucose 2% anaerobic N.D. -0.1 -1.0 -0.6 N.D.
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -0.1 -0.8 -0.9 N.D.
stress LB Anaerobic with Oxytetracycline 0.0000003125 mM N.D. -0.2 -0.3 -1.2 N.D.
carbon source carbon source D-Mannose 5mM N.D. +0.0 -0.9 -0.8 N.D.
M9 Glucose 2% aerobic N.D. -0.0 -1.0 -0.7 N.D.
M9 Glucose 2% anaerobic N.D. -0.1 -0.8 -0.7 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.4 -0.5 -0.7 N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM N.D. -0.3 -0.4 -0.9 N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.5 +0.2 -1.2 N.D.
carbon source carbon source D-Mannose 1mM N.D. +0.2 -0.3 -1.2 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.1 +0.1 -1.3 N.D.
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. -0.2 +0.5 -1.0 N.D.
phage N4_phage 0.009375 MOI TC2 N.D. +0.2 +0.6 -0.9 N.D.
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr N.D. +0.3 -0.7 +0.8 N.D.
stress Trimethoprim 0.005 mM N.D. -0.1 +0.6 +0.9 N.D.
control PBS_cage_2_mouse_1_Cecum_40 hr N.D. +0.2 +0.4 +1.1 N.D.
phage T7_phage 0.0015625 MOI TC1 N.D. +0.7 +0.4 +0.8 N.D.
phage T7_phage 0.00015625 MOI TC4 N.D. +0.7 +0.7 +0.9 N.D.
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