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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
b2250 and yfaO are separated by 278 nucleotides
yfaO and ais are separated by 38 nucleotides
ais and arnB are separated by 307 nucleotides
arnB and yfbF are separated by 3 nucleotides
b2250: b2250 - orf, hypothetical protein (VIMSS), at 2,361,755 to 2,362,297
b2250
b2251: yfaO - predicted NUDIX hydrolase (NCBI), at 2,362,576 to 2,363,001
yfaO
b2252: ais - hypothetical protein (NCBI), at 2,363,040 to 2,363,642
ais
b2253: arnB - uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase, PLP-dependent (RefSeq), at 2,363,950 to 2,365,089
arnB
b2254: yfbF - undecaprenyl phosphate-L-Ara4FN transferase (NCBI), at 2,365,093 to 2,366,061
yfbF
Group
Condition
b2250
b2251
b2252
b2253
b2254
b2250
yfaO
ais
arnB
yfbF
stress
Aluminum chloride 5 mM
-0.5
-0.2
-3.8
-0.5
-0.3
carbon source
carbon source D-Mannose 5mM
-0.2
-0.6
-0.9
-0.5
-0.9
carbon source
carbon source D-Glucose 0.25mM
+0.6
-0.0
-2.4
+0.0
-0.8
carbon source
carbon source D-Mannose 0.5mM
-0.2
-0.1
-1.6
-0.3
-0.5
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.1
-0.2
-1.1
-0.0
-0.1
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.3
-0.4
-1.4
-0.0
-0.0
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.2
+0.3
-0.9
+0.0
-0.4
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
-1.0
-0.2
-0.7
-0.0
-0.2
stress
LB Anaerobic with Spiramycin 0.00003125 mM
-0.4
-0.3
-0.9
-0.1
-0.1
stress
LB Anaerobic with Norfloxacin 0.0000000390625 mM
-0.6
+0.1
-1.2
+0.0
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.8
-0.5
-0.2
-0.6
+0.4
carbon source
carbon source D-Mannose 1mM
-0.3
-0.2
-1.5
-0.1
+0.3
stress
LB Anaerobic with Ramoplanin 0.00003125 mM
-0.3
-0.2
-1.0
+0.1
-0.3
carbon source
carbon source D-Mannose 0.5mM
-0.7
-0.2
-1.0
+0.3
-0.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.0
-0.2
-0.4
+0.3
-0.3
predation
3 hr rep1_HD100_no_dilution
-0.7
-0.1
-0.8
+0.1
+0.1
phage
R2_N4_T1_phage10
-1.2
+0.3
-0.5
+0.1
-0.1
carbon source
carbon source D-Glucose 1mM
-0.1
-0.3
-1.2
+0.1
+0.2
stress
LB Aerobic with Spiramycin 0.0000625 mM
+0.4
-0.3
-1.2
-0.0
-0.1
phage
T7_phage 0.00015625 MOI TC3
-0.6
+0.2
-0.5
-0.3
+0.3
stress
LB Aerobic with Oxytetracycline 0.0000003125 mM
+0.4
-0.3
-0.9
+0.2
-0.0
nopredatorcontrol
3 hr NoPredatorControl
-0.3
+0.4
-0.8
+0.1
+0.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.7
-0.2
+0.4
+0.6
-0.1
stress
octanol 0.08 vol%
+1.0
-0.4
-0.0
+0.1
-0.6
phage
R1_N4_T2_phage8
+0.7
+0.2
-0.8
+0.0
+0.4
stress
M9 with Methotrexate 1.98 mM 24 hr
+0.9
-0.2
+0.5
-0.1
-0.2
phage
Papa dilution 10-1 cacl2 maltose
+0.4
-0.1
+1.3
-0.1
-0.3
phage
T7_phage 0.0015625 MOI TC1
+0.7
+0.4
-0.2
+0.2
+0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.5
+0.3
+0.8
+0.3
+0.1
phage
T7_phage 0.00015625 MOI TC4
+0.9
+0.1
+0.2
+0.3
+0.6
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