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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
glpA and glpB overlap by 11 nucleotides
glpB and glpC overlap by 4 nucleotides
glpC and yfaD are separated by 192 nucleotides
yfaD and ypaA are separated by 12 nucleotides
b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297
glpA
b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546
glpB
b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733
glpC
b2244: yfaD - hypothetical protein (NCBI), at 2,354,926 to 2,355,825
yfaD
b4543: ypaA - predicted protein (NCBI), at 2,355,838 to 2,356,023
ypaA
Group
Condition
b2241
b2242
b2243
b2244
b4543
glpA
glpB
glpC
yfaD
ypaA
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-2.0
-2.1
-2.1
-0.5
+0.3
stress
LB Anaerobic with Irgasan 0.000000625 mM
-1.6
-1.3
-1.7
+0.2
-0.7
stress
LB Anaerobic with Irgasan 0.000000625 mM
-1.3
-2.0
-1.6
+0.1
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-1.7
-2.0
-1.6
+0.3
+0.6
stress
LB Anaerobic with Spiramycin 0.000125 mM
-1.3
-1.3
-1.2
-0.1
-0.5
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-1.4
-0.7
-1.6
-0.2
+0.2
stress
LB Anaerobic with Spiramycin 0.000125 mM
-1.2
-0.8
-1.1
-0.2
-0.3
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
-0.0
-0.1
-0.4
-0.3
-2.3
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.7
-0.6
-1.0
-0.1
-0.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.5
-0.6
-0.7
+0.5
-1.2
stress
LB Anaerobic with Spiramycin 0.00003125 mM
-0.8
-0.6
-0.7
-0.1
-0.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.9
-0.4
-0.8
+0.4
-0.4
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.0
-0.6
-0.5
+0.0
+0.1
stress
LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
-0.7
-0.7
-0.4
+0.4
-0.6
stress
Cephalothin 0.01 mg/ml
+0.1
-0.2
-0.4
-0.2
-1.0
nophagecontrol
R1_N4_T1_phage0
-0.3
+0.2
+0.2
-0.4
-0.9
carbon source
carbon source D-Glucose 0.25mM
-0.2
+0.3
+0.3
+0.1
-1.6
motility
inner cut, LB soft agar motility assay
-0.2
-0.4
-0.3
+0.5
-0.6
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
-0.5
-0.5
-0.4
+0.1
+0.5
motility
outer cut, LB soft agar motility assay
-1.4
+0.3
-0.8
+0.3
+1.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.2
+0.8
+0.2
-0.2
-1.0
phage
Papa dilution 10-1 cacl2 maltose
+0.6
+0.4
+0.3
-0.4
-0.7
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.6
+0.6
+0.2
-0.5
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.5
+0.4
+0.6
-0.5
-0.3
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.8
+0.8
+0.6
-0.0
-1.3
carbon source
Ying_Others16 rep B; time point 2
+0.3
+0.3
-0.5
+0.1
+1.0
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
+0.6
+0.8
+0.5
-0.3
-0.2
phage
Ste41
+0.2
+0.4
+1.0
-0.2
+0.7
phage
RB68
+0.6
+0.7
+0.3
+0.3
+0.6
phage
Ste41
+0.7
+0.4
+0.3
+0.5
+0.6
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