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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
glpT and glpA are separated by 272 nucleotides
glpA and glpB overlap by 11 nucleotides
glpB and glpC overlap by 4 nucleotides
glpC and yfaD are separated by 192 nucleotides
b2240: glpT - sn-glycerol-3-phosphate transporter (NCBI), at 2,349,038 to 2,350,396
glpT
b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297
glpA
b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546
glpB
b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733
glpC
b2244: yfaD - hypothetical protein (NCBI), at 2,354,926 to 2,355,825
yfaD
Group
Condition
b2240
b2241
b2242
b2243
b2244
glpT
glpA
glpB
glpC
yfaD
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.7
-2.0
-2.1
-2.1
-0.5
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.6
-1.7
-2.0
-1.6
+0.3
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.6
-1.3
-2.0
-1.6
+0.1
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.8
-1.6
-1.3
-1.7
+0.2
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.4
-1.4
-0.7
-1.6
-0.2
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.6
-1.3
-1.3
-1.2
-0.1
stress
LB Anaerobic with Spiramycin 0.000125 mM
+0.6
-1.2
-0.8
-1.1
-0.2
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.0
-1.0
-0.6
-0.5
+0.0
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
+0.4
-0.7
-0.6
-1.0
-0.1
stress
LB Anaerobic with Spiramycin 0.00003125 mM
+0.4
-0.8
-0.6
-0.7
-0.1
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
-0.9
-0.4
-0.8
+0.4
stress
LB Anaerobic with Spiramycin 0.00003125 mM
+0.4
-0.7
-0.4
-0.5
-0.1
motility
outer cut, LB soft agar motility assay
+0.3
-1.4
+0.3
-0.8
+0.3
carbon source
Glycolic Acid (C)
+0.7
-0.5
-0.4
-0.5
-0.3
stress
LB Anaerobic with Furaltadone 0.000015625 mM
+0.5
-0.5
-0.6
-0.7
+0.5
stress
LB Anaerobic with Doxycycline hyclate 0.000000390625 mM
+0.5
-0.7
-0.7
-0.4
+0.4
stress
LB Anaerobic with Chloramphenicol 0.000001953125 mM
+0.5
-0.4
-0.5
-0.5
+0.1
carbon source
Glycolic Acid (C)
+0.8
-0.5
-0.5
-0.3
-0.1
stress
LB Anaerobic with Chloramphenicol 0.00000390625 mM
+0.7
-0.5
-0.4
-0.4
+0.3
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.0
+0.6
+0.6
+0.2
-0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.0
+0.5
+0.4
+0.6
-0.5
phage
Ste41
-0.3
+0.2
+0.4
+1.0
-0.2
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.3
+0.6
+0.8
+0.5
-0.3
carbon source
succinate (C)
+0.9
+0.4
+0.6
+0.1
-0.3
carbon source
succinate (C)
+0.9
+0.4
+0.2
+0.3
-0.1
stress
LB Anaerobic with Amoxicillin 0.0000078125 mM
-0.2
+0.8
+0.8
+0.6
-0.0
carbon source
L-Malic (C)
+0.9
+0.5
+0.3
+0.5
-0.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.9
+0.2
+0.7
+0.0
+0.3
carbon source
L-Malic (C)
+0.9
+0.6
+0.5
+0.4
-0.1
stress
Phosphomycin 0.001 mg/ml
+11.3
+0.0
-0.1
-0.1
-0.0
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