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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
eco and mqo are separated by 714 nucleotides
mqo and yojI are separated by 217 nucleotides
yojI and alkB are separated by 75 nucleotides
alkB and ada overlap by 1 nucleotides
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776
mqo
b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637
yojI
b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363
alkB
b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427
ada
Group
Condition
b2209
b2210
b2211
b2212
b2213
eco
mqo
yojI
alkB
ada
control
PBS_cage_2_mouse_1_Cecum_40 hr
-6.9
+0.0
-0.1
-0.0
-0.3
carbon source
carbon source D-Mannose 5mM
-5.4
+0.0
+0.3
-0.3
+0.0
phage
T7_phage 0.00015625 MOI TC4
-1.5
-0.1
-1.1
-0.6
-1.2
phage
T7_phage 0.0015625 MOI TC1
-1.2
-0.2
-0.8
-0.5
-0.4
phage
Δdbn_10
-2.9
-0.2
+0.3
-0.2
-0.2
carbon source
carbon source D-Glucose 0.25mM
-1.4
-0.3
+0.1
-0.0
-1.3
stress
LB Aerobic with Hygromycin B 0.000015625 mM
-1.5
+0.0
-0.2
+0.0
-0.8
phage
RB68
-1.6
-0.4
-0.2
-0.4
+0.2
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-1.3
-0.1
+0.0
-0.6
-0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.5
-0.0
-0.4
+0.4
-1.6
motility
outer cut, LB soft agar motility assay
-1.9
-0.1
+0.2
+0.0
-0.3
phage
RB68
-1.5
-0.3
-0.5
+0.2
+0.2
stress
LB Aerobic with Trimethoprim 0.00000125 mM
-1.5
-0.0
-0.2
+0.4
-0.3
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-1.8
-0.1
-0.1
+0.0
+0.8
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.7
+0.4
-0.4
+0.3
+0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.4
+0.6
+0.5
-0.7
+0.4
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.3
+0.6
+0.2
+0.3
+0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.6
-0.1
+0.2
-0.8
+0.7
stress
Benzalkonium Chloride 0.004 mg/ml
-1.1
+0.2
+0.4
+0.9
+0.3
phage
Ste41
+0.6
-0.7
-0.4
+0.9
+0.2
vitamin
M9 with Pyridoxine HCl 5 uM and 4-Deoxypyridoxine 25 uM
+1.5
-0.1
+0.2
-0.1
-0.7
carbon source
carbon source D-Glucose 0.5mM
+1.3
+0.1
+0.3
+0.1
-0.6
control
PBS_cage_1_mouse_1_Cecum_40 hr
+1.4
-0.3
-0.5
+0.0
+0.7
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x
-0.2
+0.1
+1.5
+0.3
-0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+1.7
-0.1
+0.2
+0.6
-0.7
carbon source
carbon source D-Mannose 1mM
+1.7
-0.4
-0.0
+0.5
+0.1
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
-0.3
+0.0
+2.1
+0.3
-0.2
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x
-0.3
+0.0
+2.1
+0.4
-0.1
phage
Papa dilution 10-1 cacl2 maltose
-0.3
+0.5
+0.7
+1.1
+0.4
stress
benzenesulfonyl fluoride
+1.2
+0.2
+0.2
+0.6
+0.2
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