Fitness for 5 genes in Escherichia coli BW25113

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500 nteco and mqo are separated by 714 nucleotidesmqo and yojI are separated by 217 nucleotidesyojI and alkB are separated by 75 nucleotidesalkB and ada overlap by 1 nucleotides b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415 eco b2210: mqo - malate:quinone oxidoreductase (NCBI), at 2,303,130 to 2,304,776 mqo b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637 yojI b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363 alkB b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427 ada
Group Condition b2209 b2210 b2211 b2212 b2213
eco mqo yojI alkB ada
control PBS_cage_2_mouse_1_Cecum_40 hr -6.9 +0.0 -0.1 -0.0 -0.3
carbon source carbon source D-Mannose 5mM -5.4 +0.0 +0.3 -0.3 +0.0
phage T7_phage 0.00015625 MOI TC4 -1.5 -0.1 -1.1 -0.6 -1.2
phage T7_phage 0.0015625 MOI TC1 -1.2 -0.2 -0.8 -0.5 -0.4
phage Δdbn_10 -2.9 -0.2 +0.3 -0.2 -0.2
carbon source carbon source D-Glucose 0.25mM -1.4 -0.3 +0.1 -0.0 -1.3
stress LB Aerobic with Hygromycin B 0.000015625 mM -1.5 +0.0 -0.2 +0.0 -0.8
phage RB68 -1.6 -0.4 -0.2 -0.4 +0.2
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -1.3 -0.1 +0.0 -0.6 -0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.5 -0.0 -0.4 +0.4 -1.6
motility outer cut, LB soft agar motility assay -1.9 -0.1 +0.2 +0.0 -0.3
phage RB68 -1.5 -0.3 -0.5 +0.2 +0.2
stress LB Aerobic with Trimethoprim 0.00000125 mM -1.5 -0.0 -0.2 +0.4 -0.3
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -1.8 -0.1 -0.1 +0.0 +0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.7 +0.4 -0.4 +0.3 +0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.4 +0.6 +0.5 -0.7 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.3 +0.6 +0.2 +0.3 +0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.6 -0.1 +0.2 -0.8 +0.7
stress Benzalkonium Chloride 0.004 mg/ml -1.1 +0.2 +0.4 +0.9 +0.3
phage Ste41 +0.6 -0.7 -0.4 +0.9 +0.2
vitamin M9 with Pyridoxine HCl 5 uM and 4-Deoxypyridoxine 25 uM +1.5 -0.1 +0.2 -0.1 -0.7
carbon source carbon source D-Glucose 0.5mM +1.3 +0.1 +0.3 +0.1 -0.6
control PBS_cage_1_mouse_1_Cecum_40 hr +1.4 -0.3 -0.5 +0.0 +0.7
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.67x -0.2 +0.1 +1.5 +0.3 -0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 +1.7 -0.1 +0.2 +0.6 -0.7
carbon source carbon source D-Mannose 1mM +1.7 -0.4 -0.0 +0.5 +0.1
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x -0.3 +0.0 +2.1 +0.3 -0.2
supernatant RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8x -0.3 +0.0 +2.1 +0.4 -0.1
phage Papa dilution 10-1 cacl2 maltose -0.3 +0.5 +0.7 +1.1 +0.4
stress benzenesulfonyl fluoride +1.2 +0.2 +0.2 +0.6 +0.2
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