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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ccmF and ccmE overlap by 4 nucleotides
ccmE and ccmD overlap by 4 nucleotides
ccmD and ccmC overlap by 4 nucleotides
ccmC and ccmB are separated by 41 nucleotides
b2196: ccmF - heme lyase, CcmF subunit (NCBI), at 2,290,983 to 2,292,926
ccmF
b2197: ccmE - periplasmic heme chaperone (NCBI), at 2,292,923 to 2,293,402
ccmE
b2198: ccmD - cytochrome c biogenesis protein (NCBI), at 2,293,399 to 2,293,608
ccmD
b2199: ccmC - heme exporter subunit (NCBI), at 2,293,605 to 2,294,342
ccmC
b2200: ccmB - heme exporter subunit (NCBI), at 2,294,384 to 2,295,046
ccmB
Group
Condition
b2196
b2197
b2198
b2199
b2200
ccmF
ccmE
ccmD
ccmC
ccmB
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.8
-0.7
-0.5
-0.5
-0.4
phage
T7_phage 0.00015625 MOI TC4
+0.4
-0.7
-0.1
-1.6
-0.0
stress
toluene 2.5 vol%
-0.1
-0.1
-1.8
+0.4
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.2
+0.3
-1.7
-0.1
+0.0
carbon source
D-Serine (C)
-0.1
-0.3
-1.1
+0.3
-0.4
carbon source
carbon source D-Glucose 0.25mM
+0.3
-1.0
+0.0
-0.8
-0.1
msc
22hr_MSC_rep1_highinoculum
-0.2
+0.3
-1.4
-0.1
-0.2
phage
R2_N4_T1_phage10
-0.7
-1.0
+0.0
-0.1
+0.4
stress
Cisplatin 0.1 mg/ml
+0.4
-0.4
-0.7
+0.1
-0.7
mzaabcde
mzaABCDE
-0.1
-0.2
-1.5
+0.1
+0.6
msc
22hr_mediaonly_rep1_lowinoculum
-0.1
+0.3
-1.4
+0.3
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.5
-0.9
+0.3
-0.5
-0.3
phage
N4_phage 0.09375 MOI TC1
-0.3
+0.1
-1.3
+0.6
+0.5
phage
T7_phage 0.0015625 MOI TC1
+0.2
-0.6
+0.8
-0.6
-0.1
carbon source
a-Ketoglutaric (C)
-0.2
-0.2
-0.7
+1.0
-0.2
carbon source
acetate (C)
-0.2
+0.2
-0.5
+0.9
-0.4
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.4
+0.4
-1.4
+0.1
+0.6
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.3
-0.5
-0.1
-0.5
+1.1
carbon source
acetate (C)
+0.0
+0.2
-0.6
+1.2
-0.3
carbon source
carbon source D-Glucose 0.25mM
-0.3
-0.2
-0.3
+0.7
+0.8
stress
Benzalkonium Chloride 0.004 mg/ml
+0.3
+0.5
+0.8
+0.4
-0.9
stress
1-ethyl-3-methylimidazolium acetate 60 mM
-0.2
-0.2
+1.0
+0.6
+0.2
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
+0.1
+1.1
+0.7
+0.2
-0.6
control
PBS_cage_1_mice _Feces_22 hr
-0.1
-0.2
+1.6
+0.3
+0.3
control
PBS_cage_1_mice _Feces_40 hr
-0.2
-0.1
+1.7
-0.0
+0.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.0
+0.8
+1.1
+0.1
-0.1
control
PBS_cage_1_mice _Feces_16 hr
+0.2
-0.5
+2.2
-0.3
+0.4
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.4
-0.0
+0.8
+0.7
+1.1
phage
N4_phage 0.009375 MOI TC2
+0.2
+0.1
+1.0
+0.6
+0.5
phage
Ste41
+0.5
+0.6
+0.4
+0.8
+0.4
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