Fitness for 5 genes in Escherichia coli BW25113

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500 ntccmF and ccmE overlap by 4 nucleotidesccmE and ccmD overlap by 4 nucleotidesccmD and ccmC overlap by 4 nucleotidesccmC and ccmB are separated by 41 nucleotides b2196: ccmF - heme lyase, CcmF subunit (NCBI), at 2,290,983 to 2,292,926 ccmF b2197: ccmE - periplasmic heme chaperone (NCBI), at 2,292,923 to 2,293,402 ccmE b2198: ccmD - cytochrome c biogenesis protein (NCBI), at 2,293,399 to 2,293,608 ccmD b2199: ccmC - heme exporter subunit (NCBI), at 2,293,605 to 2,294,342 ccmC b2200: ccmB - heme exporter subunit (NCBI), at 2,294,384 to 2,295,046 ccmB
Group Condition b2196 b2197 b2198 b2199 b2200
ccmF ccmE ccmD ccmC ccmB
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.8 -0.7 -0.5 -0.5 -0.4
phage T7_phage 0.00015625 MOI TC4 +0.4 -0.7 -0.1 -1.6 -0.0
stress toluene 2.5 vol% -0.1 -0.1 -1.8 +0.4 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 +0.3 -1.7 -0.1 +0.0
carbon source D-Serine (C) -0.1 -0.3 -1.1 +0.3 -0.4
carbon source carbon source D-Glucose 0.25mM +0.3 -1.0 +0.0 -0.8 -0.1
msc 22hr_MSC_rep1_highinoculum -0.2 +0.3 -1.4 -0.1 -0.2
phage R2_N4_T1_phage10 -0.7 -1.0 +0.0 -0.1 +0.4
stress Cisplatin 0.1 mg/ml +0.4 -0.4 -0.7 +0.1 -0.7
mzaabcde mzaABCDE -0.1 -0.2 -1.5 +0.1 +0.6
msc 22hr_mediaonly_rep1_lowinoculum -0.1 +0.3 -1.4 +0.3 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 -0.9 +0.3 -0.5 -0.3
phage N4_phage 0.09375 MOI TC1 -0.3 +0.1 -1.3 +0.6 +0.5
phage T7_phage 0.0015625 MOI TC1 +0.2 -0.6 +0.8 -0.6 -0.1
carbon source a-Ketoglutaric (C) -0.2 -0.2 -0.7 +1.0 -0.2
carbon source acetate (C) -0.2 +0.2 -0.5 +0.9 -0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 +0.4 -1.4 +0.1 +0.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.3 -0.5 -0.1 -0.5 +1.1
carbon source acetate (C) +0.0 +0.2 -0.6 +1.2 -0.3
carbon source carbon source D-Glucose 0.25mM -0.3 -0.2 -0.3 +0.7 +0.8
stress Benzalkonium Chloride 0.004 mg/ml +0.3 +0.5 +0.8 +0.4 -0.9
stress 1-ethyl-3-methylimidazolium acetate 60 mM -0.2 -0.2 +1.0 +0.6 +0.2
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +0.1 +1.1 +0.7 +0.2 -0.6
control PBS_cage_1_mice _Feces_22 hr -0.1 -0.2 +1.6 +0.3 +0.3
control PBS_cage_1_mice _Feces_40 hr -0.2 -0.1 +1.7 -0.0 +0.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 +0.8 +1.1 +0.1 -0.1
control PBS_cage_1_mice _Feces_16 hr +0.2 -0.5 +2.2 -0.3 +0.4
control PBS_cage_1_mouse_1_Cecum_40 hr -0.4 -0.0 +0.8 +0.7 +1.1
phage N4_phage 0.009375 MOI TC2 +0.2 +0.1 +1.0 +0.6 +0.5
phage Ste41 +0.5 +0.6 +0.4 +0.8 +0.4
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